BMP4 localization and PCNA expression during distraction osteogenesis of the porcine mandible

Hansen, G.; Lawler, Matthew E.; Williams, W. Bradford; Troulis, Maria J.; Kaban, Leonard B.

doi:10.1016/j.ijom.2011.12.032

Cited by 7 publications

(6 citation statements)

References 39 publications

(66 reference statements)

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“…The result showed a strong positive expression of PCNA in the early stage of traction. 29 The early result was similar to the 20 days' result of this experiment. We also used a PerkinElmer to stain 2 antibodies on 1 section and observed 2 antibodies expression simultaneously.…”

Section: Discussionsupporting

confidence: 92%

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Experimental Design of Vertical Distraction Osteogenesis Using Simple 3 Screws

Jiang

Yao

2023

Journal of Craniofacial Surgery

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Distraction osteogenesis devices are complicated. To simplify these devices, we used 3 simple screws and 1 rubber band to realize the idea and analyzed histologic changes induced by mechanical forces. Ten female New Zealand white rabbits were studied. A left or right side of the mandible was randomly selected as the experimental side (ES). The unilateral mandible was distracted, and 2 fixation screws and 1 traction screw were implanted. When the traction screw was rotated downward, the opposite force made the osteotomy block move in opposite directions to increase the bone height. The control side (CS) was not processed. The results were assessed after 20 days of traction. Bone height in the ES increased by 5 mm. Toluidine blue staining showed that the number of osteoblasts per unit area on the ES was higher than that of the CS (P < 0.01). PerkinElmer showed that the expressions of proliferating cell nuclear antigen (P = 0.016) and collagen-I (P = 0.000) on the ES were higher than those on the CS. Transmission electron microscopy showed that the number of mitochondria, endoplasmic reticulum, and Golgi apparatus on the ES was significantly greater than the CS. The results confirmed that the 3 screws vertically increase the bone height. Mechanical force signals stimulate tissue activity and lead to significant cell proliferation and differentiation in the traction zone. Collagen-I may induce osteogenesis in the early stage of traction.

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Section: Discussionsupporting

confidence: 92%

“…Hansen and colleagues used pig mandibles for distraction osteogenesis and observed the expression of PCNA during the traction process. The result showed a strong positive expression of PCNA in the early stage of traction 29 . The early result was similar to the 20 days’ result of this experiment.…”

Section: Discussionsupporting

confidence: 91%

Experimental Design of Vertical Distraction Osteogenesis Using Simple 3 Screws

Jiang

Yao

2023

Journal of Craniofacial Surgery

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“…In our study, we found that Fgf8 overexpression in pre-migratory CNC cells inhibits osteogenic differentiation both in vivo and in vitro. It is well established that BMP signaling plays essential role in osteogenesis (Li et al, 1998;Shen et al, 2009;Hansen et al, 2012;Shiozaki et al, 2013). However, BMP and FGF signaling often antagonize their function each other during embryonic development and organogenesis (Neubu ¨ser et al, 1997;St Amand et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

FGF8 signaling sustains progenitor status and multipotency of cranial neural crest-derived mesenchymal cellsin vivoandin vitro

et al. 2015

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The cranial neural crest (CNC) cells play a vital role in craniofacial development and regeneration. They are multi-potent progenitors, being able to differentiate into various types of tissues. Both pre-migratory and post-migratory CNC cells are plastic, taking on diverse fates by responding to different inductive signals. However, what sustains the multipotency of CNC cells and derivatives remains largely unknown. In this study, we present evidence that FGF8 signaling is able to sustain progenitor status and multipotency of CNC-derived mesenchymal cells both in vivo and in vitro. We show that augmented FGF8 signaling in pre-migratory CNC cells prevents cell differentiation and organogenesis in the craniofacial region by maintaining their progenitor status. CNC-derived mesenchymal cells with Fgf8 overexpression or control cells in the presence of exogenous FGF8 exhibit prolonged survival, proliferation, and multi-potent differentiation capability in cell cultures. Remarkably, exogenous FGF8 also sustains the capability of CNC-derived mesenchymal cells to participate in organogenesis such as odontogenesis. Furthermore, FGF8-mediated signaling strongly promotes adipogenesis but inhibits osteogenesis of CNC-derived mesenchymal cells in vitro. Our results reveal a specific role for FGF8 in the maintenance of progenitor status and in fate determination of CNC cells, implicating a potential application in expansion and fate manipulation of CNC-derived cells in stem cell-based craniofacial regeneration.

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“…and within the inferior alveolar canal (nerve growth factor) in the middle thirddd of the wound. [45–49] Increased presence of these growth factors has been associated with more exuberant osteogenesis within these regions. [45–48, 50–53] Even without these factors, continuous DO allowed for robust bone formation at the inferior border at rates up to 4.5 mm/day.…”

Section: Discussionmentioning

confidence: 99%