Platelet-rich plasma combined with agarose as a bioactive scaffold to enhance cartilage repair: An <i>in vitro</i> study

Yin, Zhaowei; Yang, Xiaofei; Jiang, Yiqiu; Xing, Linzi; Xu, Yang; Lu, Yong; Ding, Peng; Ma, Junxin; Xu, Yan; Gui, Jian‐Fang

doi:10.1177/0885328213492573

Cited by 40 publications

(32 citation statements)

References 30 publications

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“…Platelets are a known source of several GF with beneficial effects in tissue healing 4 , including transforming growth factor-beta (TGF-β), insulin-like GF I (IGF-I), platelet-derived GF (PDGF), vascular endothelial GF (VEGF), fibroblast GF (FGF), and epidermal GF (EGF). This knowledge stimulated the development of a platelet concentrate with the purpose of increasing levels of local GF delivery at the injury site and improving the tissue repair process 5 .…”

Section: Introductionmentioning

confidence: 99%

Effects of heterologous platelet-rich plasma gel on standardized dermal wound healing in rabbits

et al. 2015

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Intellectual and scientific content of the study, surgical procedures, critical revision. ABSTRACT PURPOSE:To evaluate the potential of heterologous platelet-rich plasma (PRP) gel for surgical skin wound healing in rabbits METHODS: Blood from a single healthy dog was used for PRP production, with calcium gluconate added to the PRP to form the gel.Two surgical excisions, one to the right and the other to the left of the dorsal midline, were made in six rabbits. One side was randomly allocated to topical application of a physiological solution, and the other was allocated to treatment with heterologous PRP gel. Clinical assessments (weight, pain sensitivity, coloring, edema, hyperemia, exudation, crust, and granulation) and morphometric evaluations were performed 0, 3, 7, 10, 14, and 17 days postoperatively. Histological analysis was performed on the 17 th day. RESULTS:With the exception of the presence of a crust at day 10, clinical variables did not differ significantly between the experimental groups. In both the control and PRP-treated groups, differences were identified when comparing time-points in terms of wound area reduction. Histological results indicated no significant differences between the control group and the PRP-treated group. CONCLUSION:Heterologous platelet-rich plasma gel promoted dermal wound healing in rabbits with no adverse effects.

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Section: Introductionmentioning

confidence: 99%

Effects of heterologous platelet-rich plasma gel on standardized dermal wound healing in rabbits

et al. 2015

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“…The porous scaffold may also contain biological factors that promote cell proliferation and differentiation. 21,22 To improve the biological effects, numerous attempts have been set up to promote cell adhesion and ECM secretion of 3D PCL scaffolds by combining them with degradable bioactive materials (BMSC-derived natural ECM, 23,24 platelet-rich plasma, [26][27][28] and collagen 29 ). Our previous study testified that MC is able to regenerate cartilage by using biodegradable BMSC-derived ECM (BMSC-dECM) scaffolds.…”

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confidence: 99%

Chondrogenic Regeneration Using Bone Marrow Clots and a Porous Polycaprolactone-Hydroxyapatite Scaffold by Three-Dimensional Printing

Yao

Wei

Liu

et al. 2015

Tissue Engineering Part A

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Scaffolds play an important role in directing three-dimensional (3D) cartilage regeneration. Our recent study reported the potential advantages of bone marrow clots (MC) in promoting extracellular matrix (ECM) scaffold chondrogenic regeneration. The aim of this study is to build a new scaffold for MC, with improved characteristics in mechanics, shaping, and biodegradability, compared to our previous study. To address this issue, this study prepared a 3D porous polycaprolactone (PCL)-hydroxyapatite (HA) scaffold combined with MC (Group A), while the control group (Group B) utilized a bone marrow stem cell seeded PCL-HA scaffold. The results of in vitro cultures and in vivo implantation demonstrated that although an initial obstruction of nutrient exchange caused by large amounts of fibrin and erythrocytes led to a decrease in the ratio of live cells in Group A, these scaffolds also showed significant improvements in cell adhesion, proliferation, and chondrogenic differentiation with porous recanalization in the later culture, compared to Group B. After 4 weeks of in vivo implantation, Group A scaffolds have a superior performance in DNA content, Sox9 and RunX2 expression, cartilage lacuna-like cell and ECM accumulation, when compared to Group B. Furthermore, Group A scaffold size and mechanics were stable during in vitro and in vivo experiments, unlike the scaffolds in our previous study. Our results suggest that the combination with MC proved to be a highly efficient, reliable, and simple new method that improves the biological performance of 3D PCL-HA scaffold. The MC-PCL-HA scaffold is a candidate for future cartilage regeneration studies.Introduction D ue to the poor regenerative capacity of cartilage in vivo, articular cartilage defect is a great challenge in the field of orthopedic surgery.1 There are several cartilage repair techniques, including microfracture, joint irrigation or debridement, osteochondral grafting, and autologous chondrocyte implantation.2,3 As it is a simple, convenient, and relatively inexpensive choice, microfracture has become the preferred clinical treatment. 4 Previous evidence proved that bone marrow clots (MC) on the surface of the microfracture lesions provide an optimal environment for cartilaginous tissue repair. 5,6 However, this technique results in a repaired tissue with lower mechanical strength. This tissue is easily worn by daily activities, causing symptoms to recur and necessitating a second repair. 7,8 The poor mechanical strength, the instability of MC, and the loss of bone marrowderived mesenchymal stem cell (BMSCs) may be the main barriers to fibrocartilage repair by microfracture. 9 Although the detailed mechanisms by which MC fibrocartilage forms are not clear, the combination of MC chondrogenic differentiation and high-strength biodegradable scaffolds may provide an advanced approach to the repair and functional reconstruction of cartilage defects. 10,11The fundamental concept underlying tissue engineering is the combination of a scaffold with living cells and/o...

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“…7,8 However, the last and probably the most important mechanism of action that explains the well-documented pain reduction, which is also the most prominent and disabling symptom of knee OA, seems to be the anti-inflammatory and antinociceptive role (by regulation of nuclear factor-kappa B and cyclooxygenase-2).…”

Section: Role Of Prp In Oamentioning

confidence: 99%

Platelet-rich Plasma in Orthopedics: Efficacy, Evidence, and Evolution of Our Understanding over 10 Years

2018

Journal of Postgraduate Medicine, Education and Research

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Introduction: Platelet-rich plasma (PRP) is fast emerging as a minimally invasive treatment option in a wide variety of orthopedic conditions. We were the forerunners in India in its use in osteoarthritis (OA), lateral epicondylitis, plantar fasciitis, and sports injuries. We present our experience with prospective clinical studies and multiple research projects, and have shown some aspects to be reproducible and evidence-based, whereas some areas of application seem to demand more evidence prior to routine recommendation for use.

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Platelet-rich plasma combined with agarose as a bioactive scaffold to enhance cartilage repair: An in vitro study

Cited by 40 publications

References 30 publications

Effects of heterologous platelet-rich plasma gel on standardized dermal wound healing in rabbits

Effects of heterologous platelet-rich plasma gel on standardized dermal wound healing in rabbits

Chondrogenic Regeneration Using Bone Marrow Clots and a Porous Polycaprolactone-Hydroxyapatite Scaffold by Three-Dimensional Printing

Platelet-rich Plasma in Orthopedics: Efficacy, Evidence, and Evolution of Our Understanding over 10 Years

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