Effects of heterologous platelet-rich plasma gel on standardized dermal wound healing in rabbits

Abstract

Intellectual and scientific content of the study, surgical procedures, critical revision. ABSTRACT PURPOSE:To evaluate the potential of heterologous platelet-rich plasma (PRP) gel for surgical skin wound healing in rabbits METHODS: Blood from a single healthy dog was used for PRP production, with calcium gluconate added to the PRP to form the gel.Two surgical excisions, one to the right and the other to the left of the dorsal midline, were made in six rabbits. One side was randomly allocated to topical application of a physiological solution, and the other was allocated to treatment with heterologous PRP gel. Clinical assessments (weight, pain sensitivity, coloring, edema, hyperemia, exudation, crust, and granulation) and morphometric evaluations were performed 0, 3, 7, 10, 14, and 17 days postoperatively. Histological analysis was performed on the 17 th day. RESULTS:With the exception of the presence of a crust at day 10, clinical variables did not differ significantly between the experimental groups. In both the control and PRP-treated groups, differences were identified when comparing time-points in terms of wound area reduction. Histological results indicated no significant differences between the control group and the PRP-treated group. CONCLUSION:Heterologous platelet-rich plasma gel promoted dermal wound healing in rabbits with no adverse effects.

Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gel

“…Comparing the percentage of collagen present in the wounds of the control and treated group, we conclude that there is no statistically significant difference (p>0.05) between the groups, and therefore, the heterologous PRP gel did not affect the amount of collagen. This result corroborates Abegão et al (2015) who, while using a less accurate technique, also observed no increase in collagen by using heterologous PRP gel for wound healing in rabbits. This result corroborates Abegão et al (2015) who, while using a less accurate technique, also observed no increase in collagen by using heterologous PRP gel for wound healing in rabbits.…”

“…Comparing the percentage of collagen present in the wounds of the control and treated group, we conclude that there is no statistically significant difference Using polarized light microscopy, we observed that the collagen fibers stained with Picrosirius form a dense network characterized by the birefringent property of the collagen molecule, represented in this study by Figures 3G and 3H (VALENTI et al, 2010). Other methods that allow the observation of collagen fibers are found in the literature, such as Masson'sTrichrome staining and Hematoxylin and Eosin staining (DEROSSI et al, 2009;ABEGÃO et al, 2015); however the staining solution used in this study is considered more specific for the evaluation of collagen (LATTOUF et al, 2014). The results obtained from the quantification of collagen are shown in Figure 4.…”

“…On days 7 (M7), 14 (M14), and 17 (M17) after experimental induction of dermal wounds in the dorsal cervical region on six rabbits, called A-side control group (treated with 0,5 mL of 0.9% sodium chloride) and B-side treated group (using 0,5 mL of PRP heterologous gel obtained by double centrifugation from dogs), as previously reported by Abegão et al (2015), we clinically assessed the wound color (1-pinkish, 2-yellowish, 3-pale, 4-cyanotic), exudate, crust, edema, hyperemia, and granulation tissue (1-missing, 2-present). Morphometric analysis was carried out using graph digital caliper (DC-60 Western ® ) to measure the major and minor diameters of the wound.…”

“…Twelve 8-mm punch biopsy skin samples were obtained by Abegão et al (2015) in the 17 th day of the healing process, fixed in 10% buffered formalin solution for 24 hours, and then embedded in paraffin. In this study, serial tissue sections, 5-μm thick were prepared with a manual microtome paraffin blocks and then stained for 30 min using Picrosiriusred F3BA were sectioned for evaluation of interstitial collagen fraction (LATTOUF et al, 2014).…”

“…The heterologous PRP has been proposed as an important and safe alternative in cases where the patient has unfeasible or contraindicated conditions for obtaining their own blood (SHAN et al, 2013;SUZUKI et al, 2013;MARCK et al, 2014). In studies in our laboratory, Abegão et al (2015) reported that the heterologous PRP gel was able to promote wound healing without causing harmful effects. However, there is a need for further studies to evaluate the quality of scar tissue in wounds treated with heterologous PRP as well as to quantify the presence of collagen.…”