Platelet-Rich Plasma Ameliorates Cyclophosphamide-Induced Acute Interstitial Cystitis/Painful Bladder Syndrome in a Rat Model

Chen, Yung‐Hsiang; Man, K. Ching; Chen, Wen‐Chi; Liu, Po-Len; Tsai, Kao-Sung; Tsai, Ming-Yen; Wu, Yu‐Tzu; Chen, Huey‐Yi

doi:10.3390/diagnostics10060381

Cited by 13 publications

(16 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the expression of cell junction-associated protein zonula occludens 2 (ZO-2) was increased (p < 0.05) and the expression of IL-6 was decreased (p < 0.05) in group 4 compared to group 2. Furthermore, they showed that there was no synergistic effect when PRP and HA were administered together (p > 0.06), but rather PRP, due to its ability to express ZO-2, would play a predominant role in urothelial repair [17].…”

Section: Significant Resultsmentioning

confidence: 99%

“…The remaining plasma was centrifuged at 3000 rpm for 10 min. The supernatant was discarded, and approximately 1 mL of PRP was obtained from the buffy coat [16,17].…”

Section: Significant Resultsmentioning

confidence: 99%

“…In 2020, Chen et al [17] evaluated the efficacy of intravesical instillation with PRP and hyaluronic acid (HA) in mouse models of IC/BPS, and the possible proliferation of normal human fibroblast cells (HFCs). The authors randomized 30 virgin female rats into five groups according to the intravesical substance injected.…”

Section: Preclinical Studiesmentioning

confidence: 99%

See 2 more Smart Citations

Use of Intravesical Injections of Platelet-Rich Plasma for the Treatment of Bladder Pain Syndrome: A Comprehensive Literature Review

et al. 2021

View full text Add to dashboard Cite

Background: Bladder pain syndrome/interstitial cystitis (BPS/IC) or primary bladder pain syndrome (PBPS) is a complex and poorly understood condition. This comprehensive review aimed to discuss the potential application of platelet-rich plasma (PRP) in the treatment of BPS/IC. The pathophysiology of BPS/IC is characterized by urothelial damage that triggers a chain of events leading to chronic inflammation and other conditions. Frequently, in subjects affected by BPS/IC, recurrent urinary tract infection (rUTI) is associated with difficult therapeutic management. For these reasons, many oral and intravesical treatments (e.g., antibiotic therapy and intravesical anesthetic instillations) have been proposed to alleviate the symptoms of IC/BPS. However, the limitation of these treatments is the short duration of improvement. The purpose of this review is to analyze the efficacy of intravesical PRP injections in subjects with PBS/IC and to try to understand the potential therapeutic effects on the pathophysiology of this disease. Methods: A nonsystematic literature search using Pubmed, EMBASE, Scopus, Web of Science, Medline was performed from January 2000 to August 2021. The following terms were combined to capture relevant publications: “platelet-rich plasma”, “interstitial cystitis”, “PRP”, “bladder pain syndrome”, and “painful bladder syndrome”. Results: After exclusion of non-pertinent studies/articles, we have analyzed 5 studies. In detail, 2 articles concerned preclinical studies in which animal models were used. The authors showed an improvement in the histological pattern with less bleeding in treated subjects, a lower presence of inflammatory cytokines and an increase in the mitotic index of urothelial cells in animals treated with intravesical PRP. In the three prospective clinical trials analyzed, patients with BPS/IC who underwent monthly intravesical PRP injections were found to have a statistically significant improvement in symptoms with modulation of growth factors and inflammatory proteins. Conclusions: New evidence suggests that treatment with intravesical PRP could improve urothelial regeneration and reduces chronic inflammation in BPS/IC, modifying the clinical history of its pathology.

show abstract

Section: Significant Resultsmentioning

confidence: 99%

“…The remaining plasma was centrifuged at 3000 rpm for 10 min. The supernatant was discarded, and approximately 1 mL of PRP was obtained from the buffy coat [16,17].…”

Section: Significant Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Use of Intravesical Injections of Platelet-Rich Plasma for the Treatment of Bladder Pain Syndrome: A Comprehensive Literature Review

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Platelet-rich plasma helps not only in the management of urogynaecological problems but is handy in the diagnosis of certain conditions like painful bladder syndrome/interstitial cystitis. It has the potential to act as a modulator of urothelial repair, and this could be made useful in painful bladder syndromes [32]. Various research in the past couple of years is clearly suggestive of the fact that intravesical platelet-rich plasma reduces chronic inflammation in bladder pain syndrome/interstitial cystitis (BPS/IC) as they could improve regeneration of the urothelium [33].…”

Section: Plateletmentioning

confidence: 99%

Platelet-Rich Plasma: A Promising Regenerative Therapy in Gynecological Disorders

Varghese¹,

Acharya²

2022

Cureus

View full text Add to dashboard Cite

Platelet-rich plasma (PRP) could be understood as a special preparation of plasma in which the concentration of platelet is immensely high. This rationale for plasma use has been in the medical science for many years with plenty of success in various fields where it was inculcated, bringing dramatically favorable and better outcomes in terms of disease management and prognosis. PRP has been widely used in orthopedics from the very beginning, but in the past few years its use has been extended to other fields too, such as obstetrics and gynecology. From the very onset of the introduction of platelet-rich plasma in gynecology, there had been constant research being carried out all around the globe in order to scientifically prove and confirm its exact role in the management of gynecological problems. Regenerative medicine in gynecology was among the first areas where the platelet-rich plasma was implemented and has substantially given great results, which encouraged further extensive research to be carried out in other spectrums of gynecology. The implications of such great struggles ultimately gave way to evidence suggesting the importance of platelet-rich plasma in managing gynecological disorders like Asherman's syndrome, urinary incontinence, genital fistulas, thin endometrium, etc. This review article collectively summarizes the various use of platelet-rich plasma in gynecology.

show abstract

“…Although hyperplasia is not a typical symptom of bladder pain syndrome, chronic systemic CYP injection in mouse models is still an important approach to study the underlying pathophysiology of bladder structures. However, rat bladder is more suitable for the study of bladder function, such as leak point pressure, as its capacity is more suitable than that of the mouse [ 37 ].…”

Section: Study Diseasesmentioning

confidence: 99%

Review of Animal Models to Study Urinary Bladder Function

Shen

Chen

et al. 2021

Biology

Self Cite

View full text Add to dashboard Cite

The urinary bladder (UB) serves as a storage and elimination organ for urine. UB dysfunction can cause multiple symptoms of failure to store urine or empty the bladder, e.g., incontinence, frequent urination, and urinary retention. Treatment of these symptoms requires knowledge on bladder function, which involves physiology, pathology, and even psychology. There is no ideal animal model for the study of UB function to understand and treat associated disorders, as the complexity in humans differs from that of other species. However, several animal models are available to study a variety of other bladder disorders. Such models include animals from rodents to nonhuman primates, such as mice, rats, rabbits, felines, canines, pigs, and mini pigs. For incontinence, vaginal distention might mimic birth trauma and can be measured based on leak point pressure. Using peripheral and central models, inflammation, bladder outlet obstruction, and genetic models facilitated the study of overactive bladder. However, the larger the animal model, the more difficult the study is, due to the associated animal ethics issues, laboratory facility, and budget. This review aims at facilitating adapted animal models to study bladder function according to facility, priority, and disease.

show abstract

Platelet-Rich Plasma Ameliorates Cyclophosphamide-Induced Acute Interstitial Cystitis/Painful Bladder Syndrome in a Rat Model

Cited by 13 publications

References 31 publications

Use of Intravesical Injections of Platelet-Rich Plasma for the Treatment of Bladder Pain Syndrome: A Comprehensive Literature Review

Use of Intravesical Injections of Platelet-Rich Plasma for the Treatment of Bladder Pain Syndrome: A Comprehensive Literature Review

Platelet-Rich Plasma: A Promising Regenerative Therapy in Gynecological Disorders

Review of Animal Models to Study Urinary Bladder Function

Contact Info

Product

Resources

About