Platelet-rich fibrin: Basics of biological actions and protocol modifications

Pavlović, Voja; Ćirić, Miroslav; Jovanović, Vladimir; Trandafilović, Milena; Stojanović, Predrag

doi:10.1515/med-2021-0259

Cited by 98 publications

(115 citation statements)

References 46 publications

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“…The rational for the use of blood based local therapy relies on the finding that a blood clot improves wound healing by the release of growth factors, chemokines, and antibiotic agents from platelets [ 14 , 48 ]. An increase of platelet count in the wound was shown to enhance wound healing in an experimental setting.…”

Section: Preparation Of Platelet-rich Plasma and Platelet-rich Fibrinmentioning

confidence: 99%

“…Indeed, this method is widely used in clinical medicine. Meanwhile, several different centrifugation protocols are available, which show different enrichment in platelet and leukocyte count as recently reviewed [ 48 ]. For the preparation enriched in platelets, centrifugation of whole blood samples is used.…”

Section: Preparation Of Platelet-rich Plasma and Platelet-rich Fibrinmentioning

confidence: 99%

“…The harsher the centrifugation conditions, the more platelets will sediment in the buffy coat. According to the different protocols, leukocyte-poor platelet-rich fibrin, leukocyte-rich fibrin, and liquid platelet rich fibrin can be differentiated [ 48 , 58 ].…”

Section: Preparation Of Platelet-rich Plasma and Platelet-rich Fibrinmentioning

confidence: 99%

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Platelet-Therapeutics to Improve Tissue Regeneration and Wound Healing—Physiological Background and Methods of Preparation

et al. 2021

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Besides their function in primary hemostasis, platelets are critically involved in the physiological steps leading to wound healing and tissue repair. For this purpose, platelets have a complex set of receptors allowing the recognition, binding, and manipulation of extracellular structures and the detection of pathogens and tissue damage. Intracellular vesicles contain a huge set of mediators that can be released to the extracellular space to coordinate the action of platelets as other cell types for tissue repair. Therapeutically, the most frequent use of platelets is the intravenous application of platelet concentrates in case of thrombocytopenia or thrombocytopathy. However, there is increasing evidence that the local application of platelet-rich concentrates and platelet-rich fibrin can improve wound healing and tissue repair in various settings in medicine and dentistry. For the therapeutic use of platelets in wound healing, several preparations are available in clinical practice. In the present study we discuss the physiology and the cellular mechanisms of platelets in hemostasis and wound repair, the methods used for the preparation of platelet-rich concentrates and platelet-rich fibrin, and highlight some examples of the therapeutic use in medicine and dentistry.

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Section: Preparation Of Platelet-rich Plasma and Platelet-rich Fibrinmentioning

confidence: 99%

Section: Preparation Of Platelet-rich Plasma and Platelet-rich Fibrinmentioning

confidence: 99%

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Platelet-Therapeutics to Improve Tissue Regeneration and Wound Healing—Physiological Background and Methods of Preparation

et al. 2021

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“…Easier and successful efforts for pulp revascularization and pulp tissue regeneration were reported by using evoked bleeding (EB), where the blood clot acts as a protein scaffold and interacts with endogenous stem cells and growth factors already abundant in the adjacent bone marrow tissues [ 107 ]. The highest reported cytokines and growth factors found in PRF are IL-1 β , IL-6, IL-4, TNF- α , PDGF, VEGF, IGF-1, EGF, and transforming growth factor β 1 (TGF β 1) [ 108 ], while PRP contains FGF, PDGF, VEGF, IGF-1, EGF, and TGF β 1 [ 109 ]. The superiority of PRP came from releasing an elevated number of proteins at early time intervals whereas PRF showed a sustained production of bioactive molecules throughout a duration of 10 days [ 110 ].…”

Section: Introductionmentioning

confidence: 99%

Cell-Based Transplantation versus Cell Homing Approaches for Pulp-Dentin Complex Regeneration

Ahmed

Abouauf

AbuBakr

et al. 2021

Stem Cells International

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Regenerative dentistry has paved the way for a new era for the replacement of damaged dental tissues. Whether the causative factor is dental caries, trauma, or chemical insult, the loss of the pulp vitality constitutes one of the major health problems worldwide. Two regenerative therapies were introduced for a fully functional pulp-dentin complex regeneration, namely, cell-based (cell transplantation) and cell homing (through revascularization or homing by injection of stem cells in situ or intravenously) therapies, with each demonstrating advantages as well as drawback, especially in clinical application. The present review is aimed at elaborating on these two techniques in the treatment of irreversibly inflamed or necrotic pulp, which is aimed at regenerating a fully functional pulp-dentin complex.

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“…Fibrin is a viscoelastic biomaterial with a variable stiffness that mostly depends on the concentrations of fibrinogen and thrombin [15]. Platelet rich fibrin (PRF) is an autologus substrate prepared from blood that contains multiple biomolecules including TGFβ1 [16]. The exact composition of PRF is therefore highly variable [17].…”

Section: Introductionmentioning

confidence: 99%

Fibrin with Laminin-Nidogen Reduces Fibrosis and Improves Soft Palate Regeneration Following Palatal Injury

Salazar

Rheden

Hulzen³

et al. 2021

Biomolecules

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This study aimed to analyze the effects of fibrin constructs enhanced with laminin-nidogen, implanted in the wounded rat soft palate. Fibrin constructs with and without laminin-nidogen were implanted in 1 mm excisional wounds in the soft palate of 9-week-old rats and compared with the wounded soft palate without implantation. Collagen deposition and myofiber formation were analyzed at days 3, 7, 28 and 56 after wounding by histochemistry. In addition, immune staining was performed for a-smooth muscle actin (a-SMA), myosin heavy chain (MyHC) and paired homeobox protein 7 (Pax7). At day 56, collagen areas were smaller in both implant groups (31.25 ± 7.73% fibrin only and 21.11 ± 6.06% fibrin with laminin-nidogen)) compared to the empty wounds (38.25 ± 8.89%, p < 0.05). Moreover, the collagen area in the fibrin with laminin-nidogen group was smaller than in the fibrin only group (p ˂ 0.05). The areas of myofiber formation in the fibrin only group (31.77 ± 10.81%) and fibrin with laminin-nidogen group (43.13 ± 10.39%) were larger than in the empty wounds (28.10 ± 11.68%, p ˂ 0.05). Fibrin-based constructs with laminin-nidogen reduce fibrosis and improve muscle regeneration in the wounded soft palate. This is a promising strategy to enhance cleft soft palate repair and other severe muscle injuries.

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Platelet-rich fibrin: Basics of biological actions and protocol modifications

Cited by 98 publications

References 46 publications

Platelet-Therapeutics to Improve Tissue Regeneration and Wound Healing—Physiological Background and Methods of Preparation

Platelet-Therapeutics to Improve Tissue Regeneration and Wound Healing—Physiological Background and Methods of Preparation

Cell-Based Transplantation versus Cell Homing Approaches for Pulp-Dentin Complex Regeneration

Fibrin with Laminin-Nidogen Reduces Fibrosis and Improves Soft Palate Regeneration Following Palatal Injury

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