Fibrin with Laminin-Nidogen Reduces Fibrosis and Improves Soft Palate Regeneration Following Palatal Injury

Salazar, Doris Haydee Rosero; Rheden, René E M van; Hulzen, Manon van; Monroy, Paola L. Carvajal; Wagener, Frank A. D. T. G.; Hoff, Johannes W. Von den

doi:10.3390/biom11101547

Cited by 2 publications

(2 citation statements)

References 63 publications

(99 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They used these natural hydrogels to regenerate the soft palate and showed that they improved tissue regeneration and reduced fibrosis compared with fibrin hydrogels alone. 208 They also showed that after 56 days of wounding, collagen was deposited and myofibers were formed. Growth factors are delivered by laminin isoforms incorporated into hydrogel platforms to promote osteogenesis and neural growth, both of which are important for tissue regeneration.…”

Section: Advances In the Design Of Fibrin-based Matrices With Improve...mentioning

confidence: 98%

Technological advances in fibrin for tissue engineering

et al. 2023

View full text Add to dashboard Cite

Fibrin is a promising natural polymer that is widely used for diverse applications, such as hemostatic glue, carrier for drug and cell delivery, and matrix for tissue engineering. Despite the significant advances in the use of fibrin for bioengineering and biomedical applications, some of its characteristics must be improved for suitability for general use. For example, fibrin hydrogels tend to shrink and degrade quickly after polymerization, particularly when they contain embedded cells. In addition, their poor mechanical properties and batch-to-batch variability affect their handling, long-term stability, standardization, and reliability. One of the most widely used approaches to improve their properties has been modification of the structure and composition of fibrin hydrogels. In this review, recent advances in composite fibrin scaffolds, chemically modified fibrin hydrogels, interpenetrated polymer network (IPN) hydrogels composed of fibrin and other synthetic or natural polymers are critically reviewed, focusing on their use for tissue engineering.

show abstract

Section: Advances In the Design Of Fibrin-based Matrices With Improve...mentioning

confidence: 98%

Technological advances in fibrin for tissue engineering

et al. 2023

View full text Add to dashboard Cite

show abstract

“…PRF biomaterials are completely autologous substrates and are easy to prepare from whole blood at a minimal expense. Several types of PRF can be prepared using different protocols, enabling versatility in the application of this biomaterial in the regenerative medicine [9,10]. In PRF, a fibrin network scaffold is formed by a natural polymerization process, which is similar to the physiological architecture of the fibrin matrix.…”

Section: Introductionmentioning

confidence: 99%

Nanostructure characteristics of three types of platelet-rich fibrin biomaterial: a histological and immunohistochemical study

Nguyen-Thi,

Nguyen-Tran,

Dang-Cong

et al. 2024

Mater. Res. Express

View full text Add to dashboard Cite

AbstractBackground: Platelet-rich fibrin (PRF) is a blood-derived biomaterial that has shown potential in regenerative medicine. The objective of this study was to characterize the structure of fibrin network nanoparticles and cellular components using histological and immunohistochemical techniques. Methods: Three different types of PRF were manufactured: Choukri's platelet-rich fibrin (Ch-PRF), pure platelet-rich fibrin (P-PRF), and leukocyte platelet-rich fibrin (L-PRF), according to established protocols. The histological structures of the biomaterials were evaluated using hematoxylin and eosin staining. The fibrin network nanostructure was confirmed by Sirius Red staining and immunohistochemical staining with a fibrinogen antibody. Leukocyte components were identified by immunohistochemical staining using CD45 antibody. Results: Histological and immunohistochemical staining of the fibrin network from the PRF biomaterial revealed a natural nanostructure characterized by porous and complex branching networks. The L-PRF and Ch-PRF fibrin networks were delicate and branched, whereas the P-PRF fibrin network displayed thicker bundles of fibers that were sometimes twisted and had noticeable pores. Nonetheless, the proportion of the fibrin network area in all three types of PRF biomaterials was not significantly different. No living cells were found in the P-PRF biomaterials, whereas Ch-PRF and L-PRF contained cells. A large number of red and white blood cells were observed within the Ch-PRF fibrin network, with a non-uniform distribution. The L-PRF biomaterial possesses a uniform structure with a high density of embedded leukocytes.Conclusions: The use of peripheral blood-derived PRF biomaterials, which mimic the natural structure of fibrin nanostructures and living cell components, offers promising possibilities for tissue engineering and regenerative medicine. Additional investigation is necessary to assess the properties of PRF architecture and its practical application in medical treatment.Keywords: Peripheral blood, platelet-rich fibrin, biomaterial, scaffold, natural nanostructure.

show abstract

Fibrin with Laminin-Nidogen Reduces Fibrosis and Improves Soft Palate Regeneration Following Palatal Injury

Cited by 2 publications

References 63 publications

Technological advances in fibrin for tissue engineering

Technological advances in fibrin for tissue engineering

Nanostructure characteristics of three types of platelet-rich fibrin biomaterial: a histological and immunohistochemical study

Contact Info

Product

Resources

About