Technological advances in fibrin for tissue engineering

Sanz-Horta, Raúl; Matesanz, A.; Gallardo, Alberto; Reinecke, Helmut; Jorcano, José L.; Acedo, Pablo; Velasco, Diego; Elvira, Carlos

doi:10.1177/20417314231190288

Cited by 21 publications

(6 citation statements)

References 242 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The arrangement is also hierarchical, comprising of individual fibers tightly intertwined into larger bundles, resembling the ECM proteins, fibrin, formed during a blood clot, in both size and morphology. Naturally, fibrins facilitate the migrations of various cell types, thus they are a well-known tool in tissue engineering. While anisotropic structures are often fabricated by electrospinning with high molecular weight polymers, − much stiffer (∼ GPa) than those typically encountered by cells, here we show that similar structures can be made entirely of PEG hydrogels (∼10 s of Pa), in a self-assembled process.…”

Section: Resultsmentioning

confidence: 99%

Disodium Cromoglycate Templates Anisotropic Short-Chain PEG Hydrogels

Chen,

Luo

2024

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Anisotropic hydrogels have found widespread applications in biomedical engineering, particularly as scaffolds for tissue engineering. However, it remains a challenge to produce them using conventional fabrication methods, without specialized synthesis or equipment, such as 3D printing and unidirectional stretching. In this study, we explore the self-assembly behaviors of polyethylene glycol diacrylate (PEGDA), using disodium cromoglycate (DSCG), a lyotropic chromonic liquid crystal, as a removable template. The affinity between shortchain PEGDA (Mn = 250) and DSCG allows polymerization to take place at the DSCG surface, thereby forming anisotropic hydrogel networks with fibrin-like morphologies. This process requires considerable finesse as the phase behaviors of DSCG depend on a multitude of factors, including the weight percentage of PEGDA and DSCG, the chain length of PEGDA, and the concentration of ionic species. The key to modulating the microstructures of the all-PEG hydrogel networks is through precise control of the DSCG concentration, resulting in anisotropic mechanical properties. Using these anisotropic hydrogel networks, we demonstrate that human dermal fibroblasts are particularly sensitive to the alignment order. We find that cells exhibit a density-dependent activation pattern of a Yes-associated protein, a mechanotransducer, corroborating its role in enabling cells to translate external mechanical and morphological patterns to specific behaviors. The flexibility of modulating microstructure, along with PEG hydrogels' biocompatibility and biodegradability, underscores their potential use for tissue engineering to create functional structures with physiological morphologies.

show abstract

Section: Resultsmentioning

confidence: 99%

Disodium Cromoglycate Templates Anisotropic Short-Chain PEG Hydrogels

Chen,

Luo

2024

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…Unfortunately, fibrin lacks the mechanical strength necessary for standalone tissue regeneration. Thus, fibrin scaffolds need to be combined with synthetic materials, such as polyglycolic acid (PGA), polylactic acid (PLA), PCL, and polyvinyl alcohol (PVA), or other natural polymers, such as hyaluronic acid, alginate, or collagen [81].…”

Section: Protein-based Polymersmentioning

confidence: 99%

Evolution of Hybrid Hydrogels: Next-Generation Biomaterials for Drug Delivery and Tissue Engineering

Rana,

De la Hoz Siegler

2024

Gels

View full text Add to dashboard Cite

Hydrogels, being hydrophilic polymer networks capable of absorbing and retaining aqueous fluids, hold significant promise in biomedical applications owing to their high water content, permeability, and structural similarity to the extracellular matrix. Recent chemical advancements have bolstered their versatility, facilitating the integration of the molecules guiding cellular activities and enabling their controlled activation under time constraints. However, conventional synthetic hydrogels suffer from inherent weaknesses such as heterogeneity and network imperfections, which adversely affect their mechanical properties, diffusion rates, and biological activity. In response to these challenges, hybrid hydrogels have emerged, aiming to enhance their strength, drug release efficiency, and therapeutic effectiveness. These hybrid hydrogels, featuring improved formulations, are tailored for controlled drug release and tissue regeneration across both soft and hard tissues. The scientific community has increasingly recognized the versatile characteristics of hybrid hydrogels, particularly in the biomedical sector. This comprehensive review delves into recent advancements in hybrid hydrogel systems, covering the diverse types, modification strategies, and the integration of nano/microstructures. The discussion includes innovative fabrication techniques such as click reactions, 3D printing, and photopatterning alongside the elucidation of the release mechanisms of bioactive molecules. By addressing challenges, the review underscores diverse biomedical applications and envisages a promising future for hybrid hydrogels across various domains in the biomedical field.

show abstract

“…In this case, they are called protofibrils. Finally, protofibrils begin to assemble laterally, which leads to the formation of a native, insoluble, viscoelastic, and biocompatible fibrin hydrogel ( Bayer, 2022 ; Sanz-Horta et al, 2023 ).…”

Section: Biomaterials Used In Wound Healingmentioning

confidence: 99%

A review of the current state of natural biomaterials in wound healing applications

Ansari,

Darvishi

2024

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Skin, the largest biological organ, consists of three main parts: the epidermis, dermis, and subcutaneous tissue. Wounds are abnormal wounds in various forms, such as lacerations, burns, chronic wounds, diabetic wounds, acute wounds, and fractures. The wound healing process is dynamic, complex, and lengthy in four stages involving cells, macrophages, and growth factors. Wound dressing refers to a substance that covers the surface of a wound to prevent infection and secondary damage. Biomaterials applied in wound management have advanced significantly. Natural biomaterials are increasingly used due to their advantages including biomimicry of ECM, convenient accessibility, and involvement in native wound healing. However, there are still limitations such as low mechanical properties and expensive extraction methods. Therefore, their combination with synthetic biomaterials and/or adding bioactive agents has become an option for researchers in this field. In the present study, the stages of natural wound healing and the effect of biomaterials on its direction, type, and level will be investigated. Then, different types of polysaccharides and proteins were selected as desirable natural biomaterials, polymers as synthetic biomaterials with variable and suitable properties, and bioactive agents as effective additives. In the following, the structure of selected biomaterials, their extraction and production methods, their participation in wound healing, and quality control techniques of biomaterials-based wound dressings will be discussed.

show abstract

Technological advances in fibrin for tissue engineering

Cited by 21 publications

References 242 publications

Disodium Cromoglycate Templates Anisotropic Short-Chain PEG Hydrogels

Disodium Cromoglycate Templates Anisotropic Short-Chain PEG Hydrogels

Evolution of Hybrid Hydrogels: Next-Generation Biomaterials for Drug Delivery and Tissue Engineering

A review of the current state of natural biomaterials in wound healing applications

Contact Info

Product

Resources

About