Platelet inhibition and endothelial cell adhesion on elastin-like polypeptide surface modified materials

Blit, Patrick H.; McClung, W. Glenn; Brash, John L.; Woodhouse, Kimberly A.; Santerre, J. Paul

doi:10.1016/j.biomaterials.2011.04.067

Cited by 61 publications

(49 citation statements)

References 64 publications

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“…75 In addition to the favorable cell morphology and increased expression of endothelial nitric oxide synthase, platelet adhesion and activation were reduced compared to control PU surfaces. ECs and EPCs were shown to have adhered well to L-selectin and VE-cadherin chimera proteins that were coated on titanium surfaces.…”

mentioning

confidence: 97%

Strategies and Techniques to Enhance theIn SituEndothelialization of Small-Diameter Biodegradable Polymeric Vascular Grafts

Melchiorri

Hibino

Fisher

2013

Tissue Engineering Part B: Reviews

154

147

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mentioning

confidence: 97%

Strategies and Techniques to Enhance theIn SituEndothelialization of Small-Diameter Biodegradable Polymeric Vascular Grafts

Melchiorri

Hibino

Fisher

2013

Tissue Engineering Part B: Reviews

154

147

View full text Add to dashboard Cite

“…[19] However, such modifications have been realized mostly through lengthy processes involving chemical reactions. For example, modification with elastin-like peptide could improve the biocompatibility of PU, [20] but involved a chemical cross-linking step that took more than two days.…”

Section: Introductionmentioning

confidence: 99%

One-step surface modification of polyurethane using affinity binding peptides for enhanced fouling resistance

Wang

Zhang

et al. 2015

Journal of Biomaterials Science, Polymer Edition

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Affinity binding peptides were examined for surface fabrication of synthetic polymeric materials. Peptides possessing strong binding affinities toward polyurethane (PU) were discovered via biopanning of M13 phage peptide library. The apparent binding constant (K(app)) was as high as 2.68 × 10(9) M(-1) with surface peptide density exceeded 1.8 μg/cm(2). Structural analysis showed that the ideal peptide had a high content (75%) of H-donor amino acid residues, and that intensified hydrogen bond interaction was the key driving force for the highly stable binding of peptides on PU. PU treated with such affinity peptides promises applications as low-fouling materials, as peptides increased its wettability and substantially reduced protein adsorption and cell adhesion. These results demonstrated a facile but highly efficient one-step strategy for surface property modification of polymeric materials for biotechnological applications.

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“…The need to unravel these clinical concerns, besides the unresorbability of such metallic stents, which remain permanently in situ , has fostered numerous research groups to explore new biodegradable and bioactive polymers being able to be gradually resorbed by the body, reducing thrombosis and neointimal hyperplasia [2]. Some novel stent coating strategies have already been proposed [2, 3, 6–8]; however, these approaches are still at the preliminary stage and their long-term behavior is still unknown [3].…”

Section: Introductionmentioning

confidence: 99%

“…Some novel stent coating strategies have already been proposed [2, 3, 6–8]; however, these approaches are still at the preliminary stage and their long-term behavior is still unknown [3]. …”

Section: Introductionmentioning

confidence: 99%

“…In this scenario, relevant developments in the field of tissue engineering have recently emerged by exploiting a biomimetic approach in which biomaterials are designed to replace the internal lining of blood vessels [2]. In particular, engineered polymeric scaffolds have been functionalized to incorporate naturally inspired molecules, being able to induce specific cellular responses and guide tissue regeneration [9].…”

Section: Introductionmentioning

confidence: 99%

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Elastin-Coated Biodegradable Photopolymer Scaffolds for Tissue Engineering Applications

Barenghi

Beke

Romano

et al. 2014

BioMed Research International

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One of the main open issues in modern vascular surgery is the nonbiodegradability of implants used for stent interventions, which can lead to small caliber-related thrombosis and neointimal hyperplasia. Some new, resorbable polymeric materials have been proposed to substitute traditional stainless-steel stents, but so far they were affected by poor mechanical properties and low biocompatibility. In this respect, a new material, polypropylene fumarate (PPF), may be considered as a promising candidate to implement the development of next generation stents, due to its complete biodegradability, and excellent mechanical properties and the ease to be precisely patterned. Besides all these benefits, PPF has not been tested yet for vascular prosthesis, mainly because it proved to be almost inert, while the ability to elicit a specific biological function would be of paramount importance in such critical surgery applications. Here, we propose a biomimetic functionalization process, aimed at obtaining specific bioactivation and thus improved cell-polymer interaction. Porous PPF-based scaffolds produced by deep-UV photocuring were coated by elastin and the functionalized scaffolds were extensively characterized, revealing a stable bound between the protein and the polymer surface. Both 3T3 and HUVEC cell lines were used for in vitro tests displaying an enhancement of cells adhesion and proliferation on the functionalized scaffolds.

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Platelet inhibition and endothelial cell adhesion on elastin-like polypeptide surface modified materials

Cited by 61 publications

References 64 publications

Strategies and Techniques to Enhance theIn SituEndothelialization of Small-Diameter Biodegradable Polymeric Vascular Grafts

Strategies and Techniques to Enhance theIn SituEndothelialization of Small-Diameter Biodegradable Polymeric Vascular Grafts

One-step surface modification of polyurethane using affinity binding peptides for enhanced fouling resistance

Elastin-Coated Biodegradable Photopolymer Scaffolds for Tissue Engineering Applications

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