Platelet extracellular vesicles induce a pro‐inflammatory smooth muscle cell phenotype

Vajen, Tanja; Benedikter, Birke J.; Heinzmann, Alexandra C.A.; Vasina, Elena M.; Henskens, Yvonne; Parsons, Martin; Maguire, Patricia B.; Stassen, Frank R. M.; Heemskerk, Johan W. M.; Schurgers, Leon J.; Koenen, Rory R.

doi:10.1080/20013078.2017.1322454

Cited by 88 publications

(72 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Samples were analyzed under constant flow (flow rate = 50 AU) at 25 °C. 15 × 60 s videos were captured with a camera level of 16 as described previously . Data were analyzed using NTA 3.1.54 software with a detection threshold of 5.…”

Section: Methodsmentioning

confidence: 99%

Platelet Releasate Proteome Profiling Reveals a Core Set of Proteins with Low Variance between Healthy Adults

et al. 2018

Self Cite

View full text Add to dashboard Cite

Upon activation, platelets release a powerful cocktail of soluble and vesicular signals, collectively termed the "platelet releasate" (PR). Although several studies have used qualitative/quantitative proteomic approaches to characterize PR; with debated content and significant inter-individual variability reported, confident, and reliable insights have been hindered. Using label-free quantitative (LFQ)-proteomics analysis, a reproducible, quantifiable investigation of the 1U mL thrombin-induced PR from 32 healthy adults was conducted. MS proteomics data are available via ProteomeXchange, identifier PXD009310. Of the 894 proteins identified, 277 proteins were quantified across all donors and form a "core" PR. Bioinformatics and further LFQ-proteomic analysis revealed that the majority (84%) of "core" PR proteins overlapped with the protein composition of human platelet-derived exosomes. Vesicles in the exosomal-size range were confirmed in healthy-human PR and reduced numbers of similar-sized vesicles were observed in the PR of a mouse model of gray platelet syndrome, known to be deficient in platelet alpha-granules. Lastly, the variability of proteins in the PR was assessed, and reproducible secretion levels were found across all 32 healthy donors. Taken together, the PR contains valuable soluble and vesicular cargo and has low-population variance among healthy adults, rendering it a potentially useful platform for diagnostic fingerprinting of platelet-related disease.

show abstract

Section: Methodsmentioning

confidence: 99%

Platelet Releasate Proteome Profiling Reveals a Core Set of Proteins with Low Variance between Healthy Adults

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Intravenous injection of endothelial-derived EVs loaded with microRNA-126 prevents SMC proliferation, migration, and neointima formation in a mouse model of vascular injury. Similarly, platelet-derived EVs can induce a pro-inflammatory remodeling phenotype in SMCs, including increased migration and proliferation [9]. Systemic pressures (e.g., introduction of pathological agents) often first effect blood cells and the endothelial cells that line the blood vessel.…”

Section: Extracellular Vesicles As Mediators Of Intracellular Communimentioning

confidence: 99%

Extracellular vesicles in cardiovascular homeostasis and disease

Hutcheson

Aikawa

2018

Current Opinion in Cardiology

View full text Add to dashboard Cite

Purpose of review Extracellular vesicles (EVs) have emerged as one of the most important means through which cells interact with each other and the extracellular environment, but EV research remains challenging due to their small size and limited amount of material required for traditional molecular biology assays. The advent of new technologies and standards in the field, however, have led to increased mechanistic insight into EV function. Herein, the latest studies on the role of EVs in cardiovascular physiology and pathology are discussed. Recent findings EVs help control cardiovascular homeostasis and remodeling by mediating communication between cells and directing alterations in the extracellular matrix to respond to changes in the environment. The message carried from the parent cell to extracellular space can be intended for both local (within the same tissue) and distal (downstream of blood flow) targets. Pathological cargo loaded within EVs could further result in various diseases. On the other hand, new studies indicate that injection of EVs obtained from cultured cells into diseased tissues can promote restoration of normal tissue function. Summary EVs are an integral part of cell and tissue function, and harnessing the properties inherent to EVs may provide a therapeutic strategy to promote tissue regeneration.

show abstract

“…Samples were analyzed under constant flow conditions (flow rate = 50) at 25 °C. Fifteen 60 s videos were captured with a camera level of 16 and detection threshold of 5 as before . NTA analysis revealed a shift in the distribution of small (0–200 nm) vesicles in pregnancy.…”

Section: Resultsmentioning

confidence: 99%

“…Receiver operator characteristic (ROC), AUC, specificity, and sensitivity were calculated using the ROCR package . Particle size distribution was determined by NTA using a Malvern NanoSight NS300 (Malvern Technologies, Malvern, UK) with a high‐sensitivity sCMOS camera and a 488 nm laser as before . Data was analyzed using NTA 3.1.54 software.…”

Section: Introductionmentioning

confidence: 99%

The Platelet Releasate is Altered in Human Pregnancy

Szklanna

Parsons

Wynne

et al. 2018

Proteomics Clinical Apps

Self Cite

View full text Add to dashboard Cite

Purpose: Healthy pregnancy is characterized by an increase in platelet activation and a decrease in the number of circulating platelets with gestation. Despite this recognized importance, proteomic studies investigating platelets in healthy pregnancy have not been performed. As platelet cargo can be altered in different conditions, it is hypothesized that platelets may store a relevant and bespoke collection of molecules during pregnancy. Experimental design: Comparative label-free quantitative proteomic profiling of platelet releasates (PRs) is performed from 18 healthy pregnant and 13 non-pregnant women using an MS/MS approach. Results: Of the 723 proteins identified, 69 PR proteins are found to be differentially released from platelets in pregnancy, including proteins only expressed during pregnancy such as pregnancy-specific glycoproteins and human placental lactogen. Moreover, the population of exosomal vesicles present in the PR is also modified in pregnancy. Receiver operating characteristic analysis shows the predictive ability of 11 PR proteins to distinctly classify pregnant and nonpregnant women with an area under the curve of 0.876, a sensitivity of 88.9%, and a specificity of 84.6%. Conclusions and clinical relevance: Taken together this demonstrates that platelets and their released cargo are 'educated' in physiologic stressful conditions such as pregnancy and may represent a promising platform to study pregnancy complications.

show abstract

Platelet extracellular vesicles induce a pro‐inflammatory smooth muscle cell phenotype

Cited by 88 publications

References 61 publications

Platelet Releasate Proteome Profiling Reveals a Core Set of Proteins with Low Variance between Healthy Adults

Platelet Releasate Proteome Profiling Reveals a Core Set of Proteins with Low Variance between Healthy Adults

Extracellular vesicles in cardiovascular homeostasis and disease

The Platelet Releasate is Altered in Human Pregnancy

Contact Info

Product

Resources

About