Platelet-derived growth factor is structurally related to the putative transforming protein p28sis of simian sarcoma virus

Abstract

A partial amino acid sequence of human platelet-derived growth factor, the major mitogen in serum for cells of mesenchymal origin, has been determined. A region of 104 contiguous amino acids shows virtual identity with the predicted sequence of p28sis, the putative transforming protein of simian sarcoma virus (SSV). This similarity suggests a mechanism for transformation by SSV and other agents, involving expression of growth factors.

Consequences of Stat6 deletion on Sis/PDGF- and IL-4-induced proliferation and transcriptional activation in murine fibroblasts

“…The role of PDGF in glioma (Fleming et al, 1992), ®brosarcoma (Fleming et al, 1992), mesothelioma (Van der Meeren et al, 1993) and lung cancer (Vignaud et al, 1994) has been clearly documented. Among the PDGF isoforms, PDGF BB is the human homolog of the v-sis oncogene product Doolittle et al, 1983;Water®eld et al, 1983). Jak1, Jak2 and Tyk2 (Vignais et al, 1996), as well as Stat1, Stat3 (Sadowski et al, 1993;Silvennoinen et al, 1993), Stat5 (Novak et al, 1996;Patel et al, 1996) and Stat6 (Patel et al, 1996) have been implicated among the many PDGFR downstream e ector molecules (Claesson-Welsh, 1996).…”

Normal developing rat brain expresses a platelet-derived growth factor B chain (c-sis) mRNA truncated at the 5′ end

“…Expression of a shorter, 2.8 kb PDGF-B/c-sis transcript, which lacks the 5' UTS, has been described in cultured human umbilical vein endothelial cells (HUVECs;Fen and Daniel, 1991). PDGF-B/c-sis sequences transduced by simian sarcoma and ParodiIrgens feline sarcoma viruses also lack the translation inhibitory 5' UTS (Besmer et al, 1983;Doolittle et al, 1983;Water®eld et al, 1983). The absence of the 5' UTS is thought to contribute to a more e cient translation of the PDGF-B/c-sis gene products in these cells.…”

Oncogenes, cancer and analytical cytology

“…The final connection between oncogenes and proliferation control was made in 1983 when c-sis was shown to encode a subunit of platelet-derived growth factor, PDGF (23,132). This association was consolidated in the succeeding 2 years when the v-erb B and c-fms genes respectively, were shown to encode the intracellular domain of the receptor for epidermal growth factor (EGF) (24) and a transmembrane receptor for macrophage colony-stimulating factor (CSF 1) (103,107).…”