These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer‐reviewed by leading experts in the field, making this an essential research companion.

International audienceThe classical model of hematopoiesis established in the mouse postulates that lymphoid cells originate from a founder population of common lymphoid progenitors. Here, using a modeling approach in humanized mice, we showed that human lymphoid development stemmed from distinct populations of CD127(-) and CD127(+) early lymphoid progenitors (ELPs). Combining molecular analyses with in vitro and in vivo functional assays, we demonstrated that CD127(-) and CD127(+) ELPs emerged independently from lympho-mono-dendritic progenitors, responded differently to Notch1 signals, underwent divergent modes of lineage restriction, and displayed both common and specific differentiation potentials. Whereas CD127(-) ELPs comprised precursors of T cells, marginal zone B cells, and natural killer (NK) and innate lymphoid cells (ILCs), CD127(+) ELPs supported production of all NK cell, ILC, and B cell populations but lacked T potential. On the basis of these results, we propose a "two-family" model of human lymphoid development that differs from the prevailing model of hematopoiesis

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In Part 1 by Stuart (1960), a study was made of the growth of an unstable infinitesimal disturbance, or the decay of a finite disturbance through a stable infinitesimal disturbance to zero, in plane Poiseuille flow, and that paper gave the most important terms in a solution of the equations of motion. The greater part of the present paper is concerned with a re-formulation of this problem which readily yields the complete solution. By the same method a solution for Couette flow is obtained. This solution is only a formal one for the present because the conditions imposed in deriving the solution may not be valid for Couette flow; this flow is believed to be stable to infinitesimal disturbances of the type considered.

The presence and synthesis of c‐myc protein and mRNA in the cell cycle has been studied. We find that c‐myc mRNA is present, at equivalent levels, at all times in the cell cycle with the possible exception of mitosis. Furthermore, we demonstrate that this mRNA is transcribed in both G1 and G2 phases. An analysis of the c‐myc protein in vivo shows that de novo synthesis occurs in G1 and G2 and the protein turns over with a half‐life of approximately 20‐30 min in both phases. Furthermore, the level of c‐myc protein rapidly increases in cell populations when they re‐initiate the cell cycle, thereafter decreasing as the culture reaches quiescence. The results therefore suggest that expression of c‐myc can be rapidly modulated and that it is activated during the G0 to G1 transition, but is expressed thereafter in the cell cycle.