Platelet-derived Growth Factor-C (PDGF-C) Induces Anti-apoptotic Effects on Macrophages through Akt and Bad Phosphorylation

Son, Dain; Na, Yi Rang; Hwang, Eung Soo; Seok, Seung Hyeok

doi:10.1074/jbc.m113.508994

Cited by 38 publications

(34 citation statements)

References 78 publications

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“…Western blotting-Sorted GM-BMMs and M-BMMs were lysed and analyzed for protein expression as described earlier (24). Antilactate dehydrogenase B chain (Thermo), antiphosphofructokinse-1 (SantaCruz, TX), antiribose phosphate isomerase a (Abcam, Cambridge, UK), antitransferrin receptor (SantaCruz) and antibeta actin (SantaCruz) were used at 1/2000 dilutions.…”

Section: Methodsmentioning

confidence: 99%

Proteomic Analysis Reveals Distinct Metabolic Differences Between Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) and Macrophage Colony Stimulating Factor (M-CSF) Grown Macrophages Derived from Murine Bone Marrow Cells*

Hong

Lee

et al. 2015

Molecular & Cellular Proteomics

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Macrophages are crucial in controlling infectious agents and tissue homeostasis. Macrophages require a wide range of functional capabilities in order to fulfill distinct roles in our body, one being rapid and robust immune responses. To gain insight into macrophage plasticity and the key regulatory protein networks governing their specific functions, we performed quantitative analyses of the proteome and phosphoproteome of murine primary GM-CSF and M-CSF grown bone marrow derived macrophages (GM-BMMs and M-BMMs, respectively) using the latest isobaric tag based tandem mass tag (TMT) labeling and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Strikingly, metabolic processes emerged as a major difference between these macrophages. Specifically, GM-BMMs show significant enrichment of proteins involving glycolysis, the mevalonate pathway, and nitrogen compound biosynthesis. This evidence of enhanced glycolytic capability in GM-BMMs is particularly significant regarding their pro-inflammatory responses, because increased production of cytokines upon LPS stimulation in GM-BMMs depends on their acute glycolytic capacity. In contrast, M-BMMs up-regulate proteins involved in endocytosis, which correlates with a tendency toward homeostatic functions such as scavenging cellular debris. Together, our data describes a proteomic network that underlies the pro-inflammatory actions of GM-BMMs as well as the homeostatic functions of M-BMMs. Molecular & Cellular

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Section: Methodsmentioning

confidence: 99%

Proteomic Analysis Reveals Distinct Metabolic Differences Between Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) and Macrophage Colony Stimulating Factor (M-CSF) Grown Macrophages Derived from Murine Bone Marrow Cells*

Hong

Lee

et al. 2015

Molecular & Cellular Proteomics

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“…10,11 Although PDGF is synthesized, stored, and released by platelets upon activation, it is also produced by a plethora of other cells, including smooth muscle cells, activated macrophages such as alveolar macrophages, and vascular endothelial cells. 12 Macrophages of M2c type express high levels of cluster of differentiation 163 (CD163), which has been shown to be associated with tissue repair and remodeling of the wound and promoting cell proliferation in mice. 13 In 2004, Lau et al 14 reported that CD163 immunoreactivity was observed in resident macrophages of all normal tissue samples including lymph node and tonsil, red pulp macrophages of spleen, bone marrow, alveolar macrophages of the lung, and Kupffer cells of the liver.…”

Section: Introductionmentioning

confidence: 99%

In vivo association of immunophenotyped macrophages expressing CD163 with PDGF-B in gingival overgrowth-induced by three different categories of medications

Almahrog

Radwan

El-Zehery

et al. 2016

Journal of Oral Biology and Craniofacial Research

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“…PDGF-CC is highly expressed in the vascular system (8,14) and is produced as a secreted homodimer that binds to and activates the PDGF receptors PDGFR-α and PDGFR-β (12,15). PDGF-CC is a critical survival/protective factor for neuronal cells (8,9,16) and macrophages (17) and has been shown to be a potent angiogenic factor (10,15,(18)(19)(20).…”

mentioning

confidence: 99%

Vasoprotective effect of PDGF-CC mediated by HMOX1 rescues retinal degeneration

Zhao

Kumar

Lee

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Significance PDGF-CC plays critical roles in many biological processes, such as development, tumor growth, and angiogenesis. However, its role in blood vessel survival/regression and the underlying mechanisms remain unknown. Here, using different loss- and gain-of-function assays and multiple model systems, we show that PDGF-CC is a critical vascular protective factor required to maintain blood vessel survival. Mechanistically, we found that heme oxygenase-1 (HMOX1) activity is crucial for the vascular protective/survival effect of PDGF-CC. Given the general involvement of vascular degeneration in most degenerative diseases, PDGF-CC may be of therapeutic use in treating different types of degenerative disorders. Our findings point out that the PDGF-CC level should be monitored closely in various pathological conditions to ensure normal blood vessel survival.

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Platelet-derived Growth Factor-C (PDGF-C) Induces Anti-apoptotic Effects on Macrophages through Akt and Bad Phosphorylation

Cited by 38 publications

References 78 publications

Proteomic Analysis Reveals Distinct Metabolic Differences Between Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) and Macrophage Colony Stimulating Factor (M-CSF) Grown Macrophages Derived from Murine Bone Marrow Cells*

Proteomic Analysis Reveals Distinct Metabolic Differences Between Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) and Macrophage Colony Stimulating Factor (M-CSF) Grown Macrophages Derived from Murine Bone Marrow Cells*

In vivo association of immunophenotyped macrophages expressing CD163 with PDGF-B in gingival overgrowth-induced by three different categories of medications

Vasoprotective effect of PDGF-CC mediated by HMOX1 rescues retinal degeneration

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