Platelet Adhesion and Fibrinogen Accretion on a Family of Elastin-Like Polypeptides

Srokowski, Elizabeth M.; Blit, Patrick H.; McClung, W. Glenn; Brash, John L.; Santerre, J. Paul; Woodhouse, K. A.

doi:10.1163/092050609x12578498935594

Cited by 23 publications

(58 citation statements)

References 51 publications

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“…In a follow-up study, Woodhouse and colleagues reported platelet adhesion and fibrinogen adsorption on Mylar™ coated with three different ELPs. The authors observed a reduction in fibrinogen adsorption in all ELP-coated films; however, only the longer polypeptide chains exhibited decreased platelet adhesion (Srokowski et al, 2011). In a related study, an elastin-mimetic triblock protein polymer was evaluated by Chaikof and colleagues as a non-thrombogenic coating of impregnated ePTFE vascular grafts.…”

Section: Vascular Prostheses Passivationmentioning

confidence: 99%

“…Albumin induces less platelet adsorption than other plasma proteins such as fibrinogen, which binds to the platelet GP IIb/IIIa receptor via arginine-glycine-aspartic acid (RGD) sequences (Zaidi et al, 1996;Srokowski et al, 2011). Despite albumin's rapid adsorption to the surface of a synthetic material, more thermodynamically favored proteins replace albumin on the surface (Andrade and Hlady, 1987).…”

Section: Vascular Prostheses Passivationmentioning

confidence: 99%

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Vascular Grafting Strategies in Coronary Intervention

2014

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With the growing need for coronary revascularizations globally, several strategies to restore blood flow to the heart have been explored. Bypassing the atherosclerotic coronary arteries with autologous grafts, synthetic prostheses, and tissue-engineered vascular grafts continue to be evaluated in search of a readily available vascular graft with clinically acceptable outcomes. The development of such a vascular graft including tissue engineering approaches both in situ and in vitro is herein reviewed, facilitating a detailed comparison on the role of seeded cells in vascular graft patency.

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Section: Vascular Prostheses Passivationmentioning

confidence: 99%

Section: Vascular Prostheses Passivationmentioning

confidence: 99%

Vascular Grafting Strategies in Coronary Intervention

2014

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“…The dynamic nature of the adsorbed layer may be an important factor that contributes to its biological response [76]. Additionally, a decrease in fibrinogen accretion and platelet adhesion was observed for ELR-coated compared to uncoated surfaces, with the longest ELR coatings exhibiting lower levels of fibrinogen adsorption and platelet adhesion and therefore being the best thromboresistant coatings [77].…”

Section: Biofunctionality Control Using Elrsmentioning

confidence: 99%

Recent Contributions of Elastin-Like Recombinamers to Biomedicine and Nanotechnology

Arias¹,

Santos²,

Fernández‐Colino³

et al. 2014

CTMC

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Abstract:The emergence of the new scientific field known as nanomedicine is being catalyzed by multiple improvements in nanoscience techniques and significant progress in materials science, especially as regards the testing of novel and sophisticated biomaterials. This conjuncture has furthered the development of promising instruments in terms of detection, bioanalysis, therapy, diagnostics and imaging. Some of the most innovative new biomaterials are protein-inspired biomimetic materials in which modern biotechnology and genetic-engineering techniques complement the huge amount of information afforded by natural protein evolution to create advanced and tailor-made multifunctional molecules. Amongst these protein-based biomaterials, Elastin-like Recombinamers (ELRs) have demonstrated their enormous potential in the fields of biomedicine and nanoscience in the last few years. This broad applicability derives from their unmatched properties, particularly their recombinant and tailor-made nature, the intrinsic characteristics derived from their elastin-based origin (mainly their mechanical properties and ability to self-assemble as a result of their stimuli-responsive behavior), their proven biocompatibility and biodegradability, as well as their versatility as regards incorporating advanced chemical or recombinant modifications into the original structure that open up an almost unlimited number of multifunctional possibilities in this developing field. This article provides an updated review of the recent challenges overcome by using these recombinant biomaterials in the fields of nano-and biomedicine, ranging from nanoscale applications in surface modifications and self-assembled nanostructures to drug delivery and regenerative medicine.

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“…The preparation of reconstituted whole blood containing labeled platelets and labeled fibrinogen has been described previously [21][22][23]. Blood was collected from healthy volunteers (human ethics approval REB-04-046, McMaster University).…”

Section: Preparation Of Re-constituted Whole Bloodmentioning

confidence: 99%

Non-fouling biomaterials based on blends of polyethylene oxide copolymers and polyurethane: simultaneous measurement of platelet adhesion and fibrinogen adsorption from flowing whole blood

Tan

McClung

Brash

2012

Journal of Biomaterials Science, Polymer Edition

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Measurements of platelet adhesion and fibrinogen adsorption from flowing whole blood to a series of polyethylene oxide (PEO)-based materials were carried out. A unique experimental design was used in which both quantities were measured in the same experiment. The materials consisted of a polyurethane (PU) as a matrix into which various triblock copolymers of general structure PEO-PU-PEO were blended; the PU block was the same in all materials but the PEO blocks ranged in molecular weight from 550 to 5000. Platelets were isolated from fresh human blood and labeled with (51)Cr; purified fibrinogen was labeled with (125)I. A whole blood preparation containing these labeled species was used for the adhesion/adsorption studies. The surfaces were exposed to the flowing blood in a cone and plate device at a wall shear rate of 300 s(-1). It was found that both platelet adhesion and fibrinogen adsorption decreased with increasing copolymer content in the blends and with decreasing PEO block size for a given copolymer content. The block size effect was due probably to higher PEO surface coverage for the lower molecular weight blocks. Fibrinogen adsorption and platelet adhesion were linearly and strongly correlated. The best performing materials showed very low fibrinogen adsorption of the order of 25 ng/cm(2), and correspondingly low platelet densities around 10,000 per cm(2), i.e. fractional platelet coverage in the vicinity of 0.2%.

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Platelet Adhesion and Fibrinogen Accretion on a Family of Elastin-Like Polypeptides

Cited by 23 publications

References 51 publications

Vascular Grafting Strategies in Coronary Intervention

Vascular Grafting Strategies in Coronary Intervention

Recent Contributions of Elastin-Like Recombinamers to Biomedicine and Nanotechnology

Non-fouling biomaterials based on blends of polyethylene oxide copolymers and polyurethane: simultaneous measurement of platelet adhesion and fibrinogen adsorption from flowing whole blood

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