Platelet activation in patients with peripheral vascular disease: Reproducibility and comparability of platelet markers

Burdess, Anne; Michelsen, Annika; Brosstad, Frank; Fox, Keith A.A.; Newby, David E.; Nimmo, A. F.

doi:10.1016/j.thromres.2011.08.015

Cited by 31 publications

(18 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, flow cytometric analysis is considered a sensitive gold standard for measurement of in vivo platelet activation. We have previously shown that in patients with peripheral arterial disease, measurements of platelet–monocyte aggregates are reproducible and consistently reflect other markers of platelet and monocyte activation . In the present study, we again report comparable levels of platelet–monocyte aggregation that were reproducible between visits.…”

Section: Discussionsupporting

confidence: 86%

Cardiovascular Effects of a Novel SIRT1 Activator, SRT2104, in Otherwise Healthy Cigarette Smokers

Venkatasubramanian

Noh

Daga

et al. 2013

JAHA

View full text Add to dashboard Cite

BackgroundWe examined the effect of the oral SIRT1 activator SRT2104 on cardiovascular function in otherwise healthy cigarette smokers.Methods and ResultsTwenty‐four otherwise healthy cigarette smokers participated in a randomized double‐blind, placebo‐controlled crossover trial and received 28 days of oral SRT2104 (2.0 g/day) or matched placebo. Plasma SRT2104 concentrations, serum lipid profile, plasma fibrinolytic factors, and markers of platelet and monocyte activation were measured at baseline and at the end of each treatment period together with an assessment of forearm blood flow during intra‐arterial bradykinin, acetylcholine, and sodium nitroprusside infusions. Three hours postdose, mean plasma SRT2104 concentration was 1328±748 ng/mL after 28 days of active treatment. Compared with placebo, serum lipid profile improved during SRT2104 administration, with reductions in serum total cholesterol (−11.6±20 versus 6±21 mg/dL), low‐density lipoprotein cholesterol (−10±17 versus 3±21 mg/dL), and triglyceride (−39.8±77 versus 13.3±57 mg/dL) concentrations (P<0.05 for all). All vasodilators produced a dose‐dependent increase in blood flow (P<0.0001) that was similar during each treatment period (P>0.05 for all). No significant differences in fibrinolytic or blood flow parameters were observed between placebo and SRT2014.ConclusionsSRT2104 appears to be safe and well tolerated and associated with an improved lipid profile without demonstrable differences in vascular or platelet function in otherwise healthy cigarette smokers.Clinical Trial Registrationhttp://www.clinicaltrials.gov. Unique identifier: NCT01031108.

show abstract

Section: Discussionsupporting

confidence: 86%

Cardiovascular Effects of a Novel SIRT1 Activator, SRT2104, in Otherwise Healthy Cigarette Smokers

Venkatasubramanian

Noh

Daga

et al. 2013

JAHA

View full text Add to dashboard Cite

show abstract

“…Spontaneous activation, generating both microparticles and inducing microaggregation of platelets, occurs in type 2 diabetic patients [108], increases with age in healthy subjects [109], and is affected by blood collection and processing procedures [109, 110]. On the other hand, platelet aggregates with leukocytes are a marker of activated platelets in CVD patients [111–114], potentially reducing the platelet count in PRP. All these factors must be taken into account when evaluating data from case-control studies that compared ROS-production in unstimulated samples of disease and healthy subjects.…”

Section: Measurement Of Reactive Species In Leukocytes and Platelementioning

confidence: 99%

Measurement and Clinical Significance of Biomarkers of Oxidative Stress in Humans

Marrocco

Altieri

Peluso

2017

Oxidative Medicine and Cellular Longevity

565

500

View full text Add to dashboard Cite

Oxidative stress is the result of the imbalance between reactive oxygen species (ROS) formation and enzymatic and nonenzymatic antioxidants. Biomarkers of oxidative stress are relevant in the evaluation of the disease status and of the health-enhancing effects of antioxidants. We aim to discuss the major methodological bias of methods used for the evaluation of oxidative stress in humans. There is a lack of consensus concerning the validation, standardization, and reproducibility of methods for the measurement of the following: (1) ROS in leukocytes and platelets by flow cytometry, (2) markers based on ROS-induced modifications of lipids, DNA, and proteins, (3) enzymatic players of redox status, and (4) total antioxidant capacity of human body fluids. It has been suggested that the bias of each method could be overcome by using indexes of oxidative stress that include more than one marker. However, the choice of the markers considered in the global index should be dictated by the aim of the study and its design, as well as by the clinical relevance in the selected subjects. In conclusion, the clinical significance of biomarkers of oxidative stress in humans must come from a critical analysis of the markers that should give an overall index of redox status in particular conditions.

show abstract

“…Importantly, platelet activation is associated to elevated sCD154 and, indeed, platelet activation markers correlate with sCD154 in blood [81-83]. For this reason, serum seems inappropriate to evaluate circulating sCD154; in fact, sCD154 levels are higher in serum than in plasma, clotting resulting in increased sCD154 generation [52,79,80,84-88].…”

Section: Introductionmentioning

confidence: 99%

New frontiers for platelet CD154

Dewitte¹,

Tanga²,

Villeneuve³

et al. 2015

Exp Hematol Oncol

View full text Add to dashboard Cite

The role of platelets extends beyond hemostasis. The pivotal role of platelets in inflammation has shed new light on the natural history of conditions associated with acute or chronic inflammation. Beyond the preservation of vascular integrity, platelets are essential to tissue homeostasis and platelet-derived products are already used in the clinics. Unanticipated was the role of platelets in the adaptative immune response, allowing a renewed conceptual approach of auto-immune diseases. Platelets are also important players in cancer growth and dissemination. Platelets fulfill most of their functions through the expression of still incompletely characterized membrane-bound or soluble mediators. Among them, CD154 holds a peculiar position, as platelets represent a major source of CD154 and as CD154 contributes to most of these new platelet attributes. Here, we provide an overview of some of the new frontiers that the study of platelet CD154 is opening, in inflammation, tissue homeostasis, immune response, hematopoiesis and cancer.

show abstract

Platelet activation in patients with peripheral vascular disease: Reproducibility and comparability of platelet markers

Cited by 31 publications

References 31 publications

Cardiovascular Effects of a Novel SIRT1 Activator, SRT2104, in Otherwise Healthy Cigarette Smokers

Cardiovascular Effects of a Novel SIRT1 Activator, SRT2104, in Otherwise Healthy Cigarette Smokers

Measurement and Clinical Significance of Biomarkers of Oxidative Stress in Humans

New frontiers for platelet CD154

Contact Info

Product

Resources

About