Plastidic phosphoglucomutase and ADP-glucose pyrophosphorylase mutants impair starch synthesis in rice pollen grains and cause male sterility

Lee, Sang‐Kyu; Eom, Joon-Seob; Hwang, Seon‐Kap; Shin, Dongjin; An, Gynheung; Okita, Thomas W.; Jeon, Jong‐Seong

doi:10.1093/jxb/erw324

Cited by 98 publications

(66 citation statements)

References 77 publications

(143 reference statements)

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“…The gene was broadly expressed in all tissues, especially in immature cob and early stage of seed development (Walley et al, 2016). It was found that the plastidic phosphoglucomutase expression was up-regulated when starch began to accumulate, while its mutant impaired starch synthesis in rice pollen grains and caused male sterility (Lee et al, 2016). GRMZM2G069008 encodes a Nudix hydrolase that is widely distributed in dicots and monocots.…”

Section: Functional Annotation For Candidate Genesmentioning

confidence: 99%

The genetic architecture of amylose biosynthesis in maize kernel

Huang

Rui-dong

et al. 2017

Plant Biotechnology Journal

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SummaryStarch is the most abundant storage carbohydrate in maize kernel. The content of amylose and amylopectin confers unique properties in food processing and industrial application. Thus, the resurgent interest has been switched to the study of individual amylose or amylopectin rather than total starch, whereas the enzymatic machinery for amylose synthesis remains elusive. We took advantage of the phenotype of amylose content and the genotype of 9,007,194 single nucleotide polymorphisms from 464 inbred maize lines. The genome‐wide association study identified 27 associated loci involving 39 candidate genes that were linked to amylose content including transcription factors, glycosyltransferases, glycosidases, as well as hydrolases. Except the waxy gene that encodes the granule‐bound starch synthase, the remaining candidate genes were located in the upstream pathway of amylose synthesis, while the downstream members were already known from prior studies. The linked candidate genes could be transferred to manipulate amylose content and thus add value to maize kernel in the breeding programme.

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Section: Functional Annotation For Candidate Genesmentioning

confidence: 99%

The genetic architecture of amylose biosynthesis in maize kernel

Huang

Rui-dong

et al. 2017

Plant Biotechnology Journal

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“…For example, FLOURY ENDOSPERM4 (FLO4), encoding a pyruvate orthophosphate dikinase (PPDK; EC 2.7.9.1), was shown to regulate carbon flow from starch and lipid biosynthesis during seeding stage (Kang et al, 2005). Some other factors such as flo2, flo6, flo7, OsbZIP58, OsBT1 (ADP-glucose transporter), RPBF (rice P-box binding factor), OspPGM (plastidic phosphor-glucomutase) and RSR1 (rice starch regulator1) have also been shown to influence starch synthesis and endosperm development in rice (Fu and Xue, 2010;Lee et al, 2016;Li et al, 2017;Peng et al, 2014;She et al, 2010;Wang et al, 2013;Yamamoto et al, 2006;. Mutants of SUBSTANDARD STARCH GRAIN4 (SSG4) and SSG6 show enlarged starch grains (SGs) in the rice endosperm (Matsushima et al, 2014.…”

Section: Introductionmentioning

confidence: 99%

OsPK2 encodes a plastidic pyruvate kinase involved in rice endosperm starch synthesis, compound granule formation and grain filling

Cai

Liu

Jiao

et al. 2018

Plant Biotechnology Journal

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SummaryStarch is the main form of energy storage in higher plants. Although several enzymes and regulators of starch biosynthesis have been defined, the complete molecular machinery remains largely unknown. Screening for irregularities in endosperm formation in rice represents valuable prospect for studying starch synthesis pathway. Here, we identified a novel rice white‐core endosperm and defective grain filling mutant, ospk2, which displays significantly lower grain weight, decreased starch content and alteration of starch physicochemical properties when compared to wild‐type grains. The normal starch compound granules were drastically reduced and more single granules filled the endosperm cells of ospk2. Meanwhile, the germination rate of ospk2 seeds after 1‐year storage was observably reduced compared with wild‐type. Map‐based cloning of OsPK2 indicated that it encodes a pyruvate kinase (PK, ATP: pyruvate 2‐O‐phosphotransferase, EC 2.7.1.40), which catalyses an irreversible step of glycolysis. OsPK2 has a constitutive expression in rice and its protein localizes in chloroplasts. Enzyme assay showed that the protein product from expressed OsPK2 and the crude protein extracted from tissues of wild‐type exhibits strong PK activity; however, the mutant presented reduced protein activity. OsPK2 (PKpα1) and three other putative rice plastidic isozymes, PKpα2, PKpβ1 and PKpβ2, can interact to form heteromer. Moreover, the mutation leads to multiple metabolic disorders. Altogether, these results denote new insights into the role of OsPK2 in plant seed development, especially in starch synthesis, compound granules formation and grain filling, which would be useful for genetic improvement of high yield and rice grain quality.

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“…Lack or reduced activity of PGM has been attributed to impaired plant growth and development. 21,22 In addition, PGM along with glucose-6phosphate dehydrogenase (G6PDH) is crucial for the generation of energy and formation of nicotinamide adenine dinucleotide phosphate (NADPH), a reducing agent with a vital role in biological reactions. PGM is also an essential enzyme with major implications in metabolic pathways.…”

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confidence: 99%

Bio-catalytic nanocompartments for in situ production of glucose-6-phosphate

et al. 2017

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Cells are sophisticated biocatalytic systems driving a complex network of biochemical reactions. A bioinspired strategy to create advanced functional systems is to design confined spaces for complex enzymatic reactions by using a combination of synthetic polymer assemblies and natural cell components. Here, we developed bio-catalytic nanocompartments that contain phosphoglucomutase protected by a biomimetic polymer membrane, which was permeabilized for reactants through insertion of an engineered α-hemolysin pore protein. These bio-catalytic nanocompartments serve for production of glucose-6-phosphate, and thus possess great potential for applications in an incomplete glycolysis, pentose phosphate pathway, or in plant biological reactions.

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Plastidic phosphoglucomutase and ADP-glucose pyrophosphorylase mutants impair starch synthesis in rice pollen grains and cause male sterility

Abstract: HighlightAnalysis of rice plastidic phosphoglucomutase and ADP-glucose pyrophosphorylase mutants reveals that starch synthesis in pollen grains requires the production of glucose-1-P and ADP-glucose in the plastids.

Cited by 98 publications

References 77 publications

The genetic architecture of amylose biosynthesis in maize kernel

The genetic architecture of amylose biosynthesis in maize kernel

OsPK2 encodes a plastidic pyruvate kinase involved in rice endosperm starch synthesis, compound granule formation and grain filling

Bio-catalytic nanocompartments for in situ production of glucose-6-phosphate

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