“…Western blot analysis of rat and fish brain extracts, including midshipman, show similar expected bands of 59-63kDa (Adrio et al, 2011;Carrera et al, 2012;Gayoso et al, 2011;Goebrecht et al, 2014;manufacturer's data). This antibody stains the appropriate pattern (i.e., known CA populations of neurons and projection patterns) in all brain regions as previously documented in midshipman and other fish species (Carrera et al, 2012;Goebrecht et al, 2014;Kuscha et al, 2012;McLean and Fetcho, 2004a) and other vertebrates (e.g., Hayes et al, 2011;Nakano et al, 2009). Alternate sections from positively labeled sections incubated without primary or secondary antisera showed a complete absence of labeling.…”