Plasmonic scattering imaging of single proteins and binding kinetics

Zhang, Pengfei; Ma, Guangzhong; Wang, Dong; Wan, Zijian; Wang, Shaopeng; Tao, Nongjian

doi:10.1038/s41592-020-0947-0

Cited by 97 publications

(172 citation statements)

References 42 publications

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“… 28 Among them, plasmonics, including the localized surface plasmon resonance (LSPR) and surface plasmon polariton (SPP), have become indispensable tools for label-free detection of molecules and quantification of the binding kinetics. 29 − 32 Therefore, combining the amplification process and plasmonic detection could further increase the overall performance of biosensing. Meanwhile, the energy losses of plasmonics and the associated heat generation at the nanoscale, known as the plasmonic photothermal (PPT) effect or thermoplasmonics, could benefit a broad range of research and innovation topics, including photothermal-assisted plasmonic sensing (PTAPS).…”

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confidence: 99%

Thermoplasmonic-Assisted Cyclic Cleavage Amplification for Self-Validating Plasmonic Detection of SARS-CoV-2

et al. 2021

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The coronavirus disease 2019 (COVID-19) has penetrated every populated patch of the globe and sows destruction in our daily life. Reliable and sensitive virus sensing systems are therefore of vital importance for timely infection detection and transmission prevention. Here we present a thermoplasmonic-assisted dual-mode transducing (TP-DMT) concept, where an amplification-free-based direct viral RNA detection and an amplification-based cyclic fluorescence probe cleavage (CFPC) detection collaborated to provide a sensitive and self-validating plasmonic nanoplatform for quantifying trace amounts of SARS-CoV-2 within 30 min. In the CFPC detection, endonuclease IV recognized the synthetic abasic site and cleaved the fluorescent probes in the hybridized duplex. The nanoscale thermoplasmonic heating dehybridized the shortened fluorescent probes and facilitated the cyclical binding–cleavage–dissociation (BCD) process, which could deliver a highly sensitive amplification-based response. This TP-DMT approach was successfully validated by testing clinical COVID-19 patient samples, which indicated its potential applications in fast clinical infection screening and real-time environmental monitoring.

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confidence: 99%

Thermoplasmonic-Assisted Cyclic Cleavage Amplification for Self-Validating Plasmonic Detection of SARS-CoV-2

et al. 2021

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“…The slow dissociation rate suggests that the protein forms strong adsorption with the sensor surface. At the same time, the faster dissociation process can be translated as an easier removal by a buffer flow after molecule adsorption on the sensor surface [39,40]. As a result, the signal of α-syn-RmAb (0.15 a.u) shows a higher response than the signal of α-syn-MmAb (0.10 a.u).…”

Section: Selectivity and Sensitivity Evaluation Of Antibody With α-Synmentioning

confidence: 99%

Enhanced Plasmonic Biosensor Utilizing Paired Antibody and Label-Free Fe3O4 Nanoparticles for Highly Sensitive and Selective Detection of Parkinson’s α-Synuclein in Serum

et al. 2021

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Parkinson’s disease (PD) is an acute and progressive neurodegenerative disorder, and diagnosis of the disease at its earliest stage is of paramount importance to improve the life expectancy of patients. α-Synuclein (α-syn) is a potential biomarker for the early diagnosis of PD, and there is a great need to develop a biosensing platform that precisely detects α-syn in human body fluids. Herein, we developed a surface plasmon resonance (SPR) biosensor based on the label-free iron oxide nanoparticles (Fe3O4 NPs) and paired antibody for the highly sensitive and selective detection of α-syn in serum samples. The sensitivity of the SPR platform is enhanced significantly by directly depositing Fe3O4 NPs on the Au surface at a high density to increase the decay length of the evanescent field on the Au film. Moreover, the utilization of rabbit-type monoclonal antibody (α-syn-RmAb) immobilized on Au films allows the SPR platform to have a high affinity-selectivity binding performance compared to mouse-type monoclonal antibodies as a common bioreceptor for capturing α-syn molecules. As a result, the current platform has a detection limit of 5.6 fg/mL, which is 20,000-fold lower than that of commercial ELISA. The improved sensor chip can also be easily regenerated to repeat the α-syn measurement with the same sensitivity. Furthermore, the SPR sensor was applied to the direct analysis of α-syn in serum samples. By using a format of paired α-syn-RmAb, the SPR sensor provides a recovery rate in the range from 94.5% to 104.3% to detect the α-syn in diluted serum samples precisely. This work demonstrates a highly sensitive and selective quantification approach to detect α-syn in human biofluids and paves the way for the future development in the early diagnosis of PD.

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“…In this sense, plasmonic single-molecule approaches mainly rely upon the extinction spectra of metallic nanoparticles (NPs) and the capacity of optical nanoapertures (i.e., arrays of nanoholes, nanopores, nanowells) to confine the electromagnetic field into ultrasmall subwavelength dimensions [ 1 , 8 , 9 , 19 , 20 , 21 , 22 , 23 ]. Both types of plasmonic nanostructures have been successfully applied to the label-free quantification of single-proteins in the diagnosis of acute and chronic diseases in early stages, the recognition of single-nucleotide specificity and the tracking of independent biological processes in living cells inserted in nanopores [ 24 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Plasmonic Biosensors for Single-Molecule Biomedical Analysis

Mauriz

Lechuga

2021

Biosensors

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The rapid spread of epidemic diseases (i.e., coronavirus disease 2019 (COVID-19)) has contributed to focus global attention on the diagnosis of medical conditions by ultrasensitive detection methods. To overcome this challenge, increasing efforts have been driven towards the development of single-molecule analytical platforms. In this context, recent progress in plasmonic biosensing has enabled the design of novel detection strategies capable of targeting individual molecules while evaluating their binding affinity and biological interactions. This review compiles the latest advances in plasmonic technologies for monitoring clinically relevant biomarkers at the single-molecule level. Functional applications are discussed according to plasmonic sensing modes based on either nanoapertures or nanoparticle approaches. A special focus was devoted to new analytical developments involving a wide variety of analytes (e.g., proteins, living cells, nucleic acids and viruses). The utility of plasmonic-based single-molecule analysis for personalized medicine, considering technological limitations and future prospects, is also overviewed.

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Plasmonic scattering imaging of single proteins and binding kinetics

Cited by 97 publications

References 42 publications

Thermoplasmonic-Assisted Cyclic Cleavage Amplification for Self-Validating Plasmonic Detection of SARS-CoV-2

Thermoplasmonic-Assisted Cyclic Cleavage Amplification for Self-Validating Plasmonic Detection of SARS-CoV-2

Enhanced Plasmonic Biosensor Utilizing Paired Antibody and Label-Free Fe3O4 Nanoparticles for Highly Sensitive and Selective Detection of Parkinson’s α-Synuclein in Serum

Plasmonic Biosensors for Single-Molecule Biomedical Analysis

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