Plasmonic nucleotide hybridization chip for attomolar detection: localized gold and tagged core/shell nanomaterials

Mubarak, Zainab H. Al; Premaratne, Gayan; Dharmaratne, Asantha C.; Mohammadparast, Farshid; Andiappan, Marimuthu; Krishnan, Sadagopan

doi:10.1039/c9lc01150a

Cited by 12 publications

(8 citation statements)

References 41 publications

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“…A plasmonic detection incorporating AuNP exhibited LSPR, and core@shell Fe 3 O 4 @Au NPs as a label with sandwich hybridization assay is reported for This journal is © The Royal Society of Chemistry 2021 miR-155 quantification at the aM level. 261 Magnetic core@shell of larger size of B105 nm size enhances the SPR signal due to its higher surface tunability. An SPRi array substrate is fabricated with 50 nm AuNPs immobilized by the SAM layer.…”

Section: Clinical Diagnosticsmentioning

confidence: 99%

Attomolar analyte sensing techniques (AttoSens): a review on a decade of progress on chemical and biosensing nanoplatforms

et al. 2021

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Section: Clinical Diagnosticsmentioning

confidence: 99%

Attomolar analyte sensing techniques (AttoSens): a review on a decade of progress on chemical and biosensing nanoplatforms

et al. 2021

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“…Subsequently, a single-stranded DNA (ssDNA) functionalised with a silver nanocube (AgNC) hybridised first with the captured miRNA, and then the ssDNA-coated Au nanoparticles were assembled on the surface of AgNC, forming Auon-Ag heterostructures, which are essential labels capable of realising an amplified SPR response [104]. Recently, an attomolar detection of miRNA-155 was achieved due to the incorporation of core/shell Fe 3 O 4 @Au nanoparticles [105].…”

Section: Lspr Biosensors For the Detection Of Mirna Biomarkersmentioning

confidence: 99%

Plasmonic Biosensors for the Detection of Lung Cancer Biomarkers: A Review

et al. 2021

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Lung cancer is the most common and deadliest cancer type globally. Its early diagnosis can guarantee a five-year survival rate. Unfortunately, application of the available diagnosis methods such as computed tomography, chest radiograph, magnetic resonance imaging (MRI), ultrasound, low-dose CT scan, bone scans, positron emission tomography (PET), and biopsy is hindered due to one or more problems, such as phenotypic properties of tumours that prevent early detection, invasiveness, expensiveness, and time consumption. Detection of lung cancer biomarkers using a biosensor is reported to solve the problems. Among biosensors, optical biosensors attract greater attention due to being ultra-sensitive, free from electromagnetic interference, capable of wide dynamic range detection, free from the requirement of a reference electrode, free from electrical hazards, highly stable, capable of multiplexing detection, and having the potential for more information content than electrical transducers. Inspired by promising features of plasmonic sensors, including surface plasmon resonance (SPR), localised surface plasmon resonance (LSPR), and surface enhanced Raman scattering (SERS) such as ultra-sensitivity, single particle/molecular level detection capability, multiplexing capability, photostability, real-time measurement, label-free measurement, room temperature operation, naked-eye readability, and the ease of miniaturisation without sophisticated sensor chip fabrication and instrumentation, numerous plasmonic sensors for the detection of lung cancer biomarkers have been investigated. In this review, the principle plasmonic sensor is explained. In addition, novel strategies and modifications adopted for the detection of lung cancer biomarkers such as miRNA, carcinoembryonic antigen (CEA), cytokeratins, and volatile organic compounds (VOCs) using plasmonic sensors are also reported. Furthermore, the challenges and prospects of the plasmonic biosensors for the detection of lung cancer biomarkers are highlighted.

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“…A new hotspot in miRNA detection is the fusion of materials science and medicine. 7 − 9 The feasibility of this hotspot relies on the signal response of materials and the complementary base pairing between the probe molecule and the target miRNA. The signal response of materials is based on various types of principles such as fluorescence, surface-enhanced Raman spectroscopy, colorimetry, and electrochemistry.…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, they have problems in low sensitivity and specificity, poor portability, and long detection period. A new hotspot in miRNA detection is the fusion of materials science and medicine. − The feasibility of this hotspot relies on the signal response of materials and the complementary base pairing between the probe molecule and the target miRNA. The signal response of materials is based on various types of principles such as fluorescence, surface-enhanced Raman spectroscopy, colorimetry, and electrochemistry. − Comparatively, the fluorescence method has received more attention due to its characteristics of high sensitivity and convenient analysis. , For instance, Chinnappan et al reported the detection of breast cancer miRNA sequences based on the change in fluorescence intensity caused by the change in the distance between the fluorophore and the quencher that was triggered by the target miRNA .…”

Section: Introductionmentioning

confidence: 99%

Ratiometric Fluorescent Biosensor Based on Forster Resonance Energy Transfer between Carbon Dots and Acridine Orange for miRNA Analysis

et al. 2021

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The expression level of miRNA is highly correlated with the pathological process of malignant tumors. Therefore, the abnormal expression of miRNA in serum is considered as reliable evidence for the existence of tumor cells. Here, a ratiometric fluorescent biosensor based on the Forster resonance energy transfer between fluorophores is proposed for detecting colorectal cancer-specific miRNA (miR-92a-3p). The miRNA in serum was first isolated by carboxyl-modified SiO 2 microspheres. Then, the addition of miRNA to the detection system resulted in the distance change between the donor acridine orange (AO) and the acceptor fluorescent carbon dots (CDs), which made the fluorescence signal change. The physicochemical properties, especially the fluorescence characteristics of CDs and AO, which enabled the ratiometric fluorescence detection, were comprehensively studied. The ratiometric fluorescent biosensor could detect miRNA in the concentration range of 1–9 nM and showed a detection limit of 0.14 nM. Moreover, the ratiometric fluorescent biosensor exhibited high selectivity for the target miRNA. The validity of the ratiometric fluorescent biosensor was also verified using the serum sample, demonstrating its potential for enzyme-free miRNA analysis.

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Plasmonic nucleotide hybridization chip for attomolar detection: localized gold and tagged core/shell nanomaterials

Abstract: We report a large surface plasmon signal amplification for a double hybridization microarray chip assembly that bridges localized gold and detection probe-carrying-core/shell Fe3O4@Au nanoparticles to enable detection of 80 aM miRNA-155 in solution.

Cited by 12 publications

References 41 publications

Attomolar analyte sensing techniques (AttoSens): a review on a decade of progress on chemical and biosensing nanoplatforms

Attomolar analyte sensing techniques (AttoSens): a review on a decade of progress on chemical and biosensing nanoplatforms

Plasmonic Biosensors for the Detection of Lung Cancer Biomarkers: A Review

Ratiometric Fluorescent Biosensor Based on Forster Resonance Energy Transfer between Carbon Dots and Acridine Orange for miRNA Analysis

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