Plasmonic nanostructure-enhanced Raman scattering for detection of SARS-CoV-2 nucleocapsid protein and spike protein variants

Yeh, Yi-Jui; Le, Trong‐Nghia; Hsiao, Wesley Wei-Wen; Tung, Kuo‐Lun; Ostrikov, Kostya; Chiang, Wei‐Hung

doi:10.1016/j.aca.2022.340651

Cited by 30 publications

(27 citation statements)

References 46 publications

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“…212 An LOD of 1 fg mL −1 and 0.1 pg mL −1 was noted for the S and N spike proteins, respectively. 212…”

Section: Plasmonic Biosensorsmentioning

confidence: 94%

“…The LSPR sensing scheme of this device is based on Ag nanoparticles with an average diameter of 22.47 nm and an aspect ratio of 1.15 on a glass substrate 211 . A three-dimensional porous microplasmaengineered nanoassemblies (AgMEN) fabricated to provide a high sensitivity to SARS-CoV-2 S variants, including wild-type, Alpha, Delta, and Omicron detection via the remarkable SERS signal collection 212 . A LOD of 1 fg/mL and 0.1 pg/mL was noted for the S and N spike proteins, respectively 212 .…”

Section: Metallic Nanoparticles For Sars-cov-2 Detectionmentioning

confidence: 99%

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Plasmonic and metamaterial biosensors: a game-changer for virus detection

Wang

Kotsifaki

2023

Sens. Diagn.

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“…212 An LOD of 1 fg mL −1 and 0.1 pg mL −1 was noted for the S and N spike proteins, respectively. 212…”

Section: Plasmonic Biosensorsmentioning

confidence: 94%

Section: Metallic Nanoparticles For Sars-cov-2 Detectionmentioning

confidence: 99%

Plasmonic and metamaterial biosensors: a game-changer for virus detection

Wang

Kotsifaki

2023

Sens. Diagn.

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show abstract

“…This work also studied Raman modes of the spike protein theoretically and established a database [ 270 ]. Different variants of the SARS-CoV-2 virus, including wild-type, Alpha, Delta, and Omicron, were successfully identified using specific antibody-tagged 3D porous Ag-based SERS substrates [ 271 ]. SERS has also shown the potential of simultaneous detection of influenza virus (H1N1), SARS-CoV-2, and respiratory syncytial virus by using magnetic-tags-based SERS substrates with extended studies in throat swabs [ 272 ].…”

Section: Sers For Disease Diagnosismentioning

confidence: 99%

Recent Trends in SERS-Based Plasmonic Sensors for Disease Diagnostics, Biomolecules Detection, and Machine Learning Techniques

2023

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Surface-enhanced Raman spectroscopy/scattering (SERS) has evolved into a popular tool for applications in biology and medicine owing to its ease-of-use, non-destructive, and label-free approach. Advances in plasmonics and instrumentation have enabled the realization of SERS’s full potential for the trace detection of biomolecules, disease diagnostics, and monitoring. We provide a brief review on the recent developments in the SERS technique for biosensing applications, with a particular focus on machine learning techniques used for the same. Initially, the article discusses the need for plasmonic sensors in biology and the advantage of SERS over existing techniques. In the later sections, the applications are organized as SERS-based biosensing for disease diagnosis focusing on cancer identification and respiratory diseases, including the recent SARS-CoV-2 detection. We then discuss progress in sensing microorganisms, such as bacteria, with a particular focus on plasmonic sensors for detecting biohazardous materials in view of homeland security. At the end of the article, we focus on machine learning techniques for the (a) identification, (b) classification, and (c) quantification in SERS for biology applications. The review covers the work from 2010 onwards, and the language is simplified to suit the needs of the interdisciplinary audience.

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“…The study employed aptamers coupled on a silver-based nanoforest surface prepared by sputtering technique for detecting recombinant spike protein by monitoring the Raman variations after protein-aptamer complex formation. Chiang et al have developed a paper-based SERS substrate which can facilitate detection of SARS-CoV-2 variants which includes omicron, delta, and alpha (Yeh et al 2023 ). The microplasma-engineered nanoassemblies fabricated on cellulose paper substrate were effective in enhancing the Raman signatures of spike proteins appearing at 2895 and 2946 cm −1 .…”

Section: Sars-cov-2 Detectionmentioning

confidence: 99%

Raman spectroscopy for viral diagnostics

et al. 2023

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Raman spectroscopy offers the potential for fingerprinting biological molecules at ultra-low concentration and therefore has potential for the detection of viruses. Here we review various Raman techniques employed for the investigation of viruses. Different Raman techniques are discussed including conventional Raman spectroscopy, surface-enhanced Raman spectroscopy, Raman tweezer, tip-enhanced Raman Spectroscopy, and coherent anti-Stokes Raman scattering. Surface-enhanced Raman scattering can play an essential role in viral detection by multiplexing nanotechnology, microfluidics, and machine learning for ensuring spectral reproducibility and efficient workflow in sample processing and detection. The application of these techniques to diagnose the SARS-CoV-2 virus is also reviewed. Graphical abstract

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Plasmonic nanostructure-enhanced Raman scattering for detection of SARS-CoV-2 nucleocapsid protein and spike protein variants

Cited by 30 publications

References 46 publications

Plasmonic and metamaterial biosensors: a game-changer for virus detection

Plasmonic and metamaterial biosensors: a game-changer for virus detection

Recent Trends in SERS-Based Plasmonic Sensors for Disease Diagnostics, Biomolecules Detection, and Machine Learning Techniques

Raman spectroscopy for viral diagnostics

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