Plasmonic metal scattering immunoassay by total internal reflection scattering microscopy with nanoscale lateral resolution

Lee, Seung‐Ah; Yu, Hyunung; Kang, Seong Ho

doi:10.1039/c4cc07665f

Cited by 12 publications

(6 citation statements)

References 27 publications

(20 reference statements)

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“…SEM data show capsaicin layer formation on the surface of gold nanospots, and AFM data show increased height of gold nanospots after conjugating with CAP. The distance between GNP and CAP was calculated from AFM data that correlate with previously reported AFM results . The resultant fluorescence enhancement was observed to be around 100 times greater than the original fluorescence signal of the CAP (Figure S6).…”

Section: Resultssupporting

confidence: 66%

“…The distance between GNP and CAP was calculated from AFM data that correlate with previously reported AFM results. 34 The resultant fluorescence enhancement was observed to be around 100 times greater than the original fluorescence signal of the CAP (Figure S6). The enhanced florescence was compared with that of highly stable, silicacoated magnetic nanoparticles conjugated with rhodamine B isothiocyanate dye (MNP@SiO 2 RITC), 35 as the fluorescence spectra matches that of capsaicin.…”

Section: Acs Sensorsmentioning

confidence: 94%

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Plasmon-Amplified Endogenous Fluorescence Nanospectroscopic Sensor Based on Inherent Elastic Scattering for Ultratrace Ratiometric Detection of Capsaicinoids

Chakkarapani

Lee

Park³

et al. 2019

ACS Sens.

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Endogenous fluorescence imaging techniques are key for modern single-molecule quantification without the use of additional labeling probes. However, the drawback of weak fluorescence signal is the primary challenge in meeting the ever-increasing demands of single-molecule detection.Here, we report a simple and reliable method that provides up to ∼100-fold uniform fluorescence enhancement of endogenous fluorescence of the capsaicinoid molecule. The method is based on a single nanoparticle plasmon-amplified endogenous fluorescence nanospectroscopic sensor (PAEFS). This work demonstrated the applicability of PAEFS in refining sensitivity at the single-molecule level by showing ultralow limits of detection (10 6 times lower than previous reports) of fluorescence-based capsaicinoids with a wide range of linear response (18 zM to 85 pM). Spectrally overlapped capsaicinoid analogues were quantified ratiometrically to detect the analogue percentages in real samples. The novel endogenous fluorescence enhancement approach presented here represents a universal sensor for enhanced detection of single molecules using existing techniques without altering the original molecular features or using add-on labeling probes.

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Section: Resultssupporting

confidence: 66%

Section: Acs Sensorsmentioning

confidence: 94%

Plasmon-Amplified Endogenous Fluorescence Nanospectroscopic Sensor Based on Inherent Elastic Scattering for Ultratrace Ratiometric Detection of Capsaicinoids

Chakkarapani

Lee

Park³

et al. 2019

ACS Sens.

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“…Therefore, an innovative, simple, cost-effective, and sensitive ACP assay method is urgently required to meet the current demand for onsite applications. In comparison to electrochemical, 13 electrochemiluminescence, 14 chemiluminescence, 15 plasmonic, 16,17 and fluorescence 18 detection methods, virtual instrument-free colorimetric detection 19 simplicity, ease of use in real-life situations in remote areas, comparative cost-effectiveness, and no requirement for trained personnel, or sophisticated instrumentation. 20,21 Over the last decade, advances in nanoscience and nanotechnology have opened an alternative opportunity to use nanomaterials as an enzyme, and recent efforts have been invested in nanozymatic-based colorimetric immunoassays with the use of different nanomaterials rather than natural enzymes.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Therefore, an innovative, simple, cost-effective, and sensitive ACP assay method is urgently required to meet the current demand for onsite applications. In comparison to electrochemical, electrochemiluminescence, chemiluminescence, plasmonic, , and fluorescence detection methods, virtual instrument-free colorimetric detection strategies have garnered significant research interest because of their simplicity, ease of use in real-life situations in remote areas, comparative cost-effectiveness, and no requirement for trained personnel, or sophisticated instrumentation. , …”

Section: Introductionmentioning

confidence: 99%

In Situ Enzymatic Generation of Gold Nanoparticles for Nanozymatic Label-free Detection of Acid Phosphatase

Ahmed

Chen

2020

ACS Appl. Nano Mater.

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Pronounced changes of acid phosphatase (ACP) in the human body may occur because of several diseases. Unusual ACP expressions might be diagnostically useful as serological and histological biomarkers for specific conditions and for investigations into the pathophysiology of their associated diseases. Recently, artificial enzyme-based colorimetric detection has garnered increased research interest because of its practicability, cost-effectiveness, and flexibility in use. Herein, for the first time, a label-free sensitive visual detection of ACP is proposed based on a signal transduction strategy in conjunction with an enzyme cascade reaction and the nanozymatic activity of the in situ synthesized gold nanoparticles (Au NPs). The ACP-catalyzed hydrolysis of L-ascorbic acid 2-phosphate sesquimagnesium (AAPS) generates ascorbic acid (AA), which in turn reacts with HAuCl 4 to generate Au NPs. The nonenzymatic activity of the synthesized Au NPs toward 3,3′,5,5′-tetramethylbenzidine (TMB) and H 2 O 2 produces a visual readout color that may be utilized to effectively determine ACP concentrations in biological media. The terephthalic acid test confirmed that the size of the Au NPs played a significant role in the nonenzymatic reaction. The quantitative sensing of ACP activity over a wide linear range (0.01−50 mU mL −1 ) with a detection limit of 0.03 mU mL −1 was realized, which endowed the proposed method with high sensitivity for potential real-life applications. Moreover, the present detection approach demonstrated higher sensitivity than a commercially available ACP kit. The approach reported in this study opens a prospect for label-free amplified colorimetric assays of enzymes and biomarkers.

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“…Plasmonic nanometals have been detected and imaged with a variety of techniques including bright-field microscopy, , dark-field microscopy, , photothermal interference contrast techniques, − differential interference contrast microscopy, , confocal Raman microscopy, and dynamic surface-enhanced Raman spectroscopy . In particular, total internal reflection scattering (TIRS) spectroscopy is one of the most powerful techniques for highly sensitive imaging with greatly reduced background noise, strong signals, and high angular resolution. − Kang et al introduced optically controlled TIRS and localization of molecules along the axial-direction in an immunoassay using TIRS. However, localization along the lateral-direction of a confined nanoscale region using TIRS-based super-resolution microscopy (TIRS-SRM) at subdiffraction limit resolution has not yet been reported for nanosensors or on biochips.…”

mentioning

confidence: 99%

Ultrasensitive Detection of α-Fetoprotein by Total Internal Reflection Scattering-Based Super-Resolution Microscopy for Superlocalization of Nano-Immunoplasmonics

Ahn¹,

Zhang²,

et al. 2016

Anal. Chem.

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Superlocalization of immunoplasmonic nanotags on antibody-bound gold-nanoislands (GNIs) along the x and y coordinates was determined using total internal reflection scattering-based super-resolution microscopy (TIRS-SRM) at subdiffraction limit resolution. Individual immunoplasmonic nanotags (20 nm silver nanoparticles) and 100 nm GNIs were selectively acquired in the evanescent field layer by wavelength-dependent plasmonic scattering using two illumination lasers (405 and 635 nm, respectively). α-Fetoprotein (AFP), a liver cancer-related model protein, was immobilized as a target molecule on the GNI arrays. The centroid position of a localized immunoplasmonic nanotag on the GNI was resolved at less than 10 nm of spatial resolution by applying 2D Gaussian fitting to its point spread function. This method showed enhanced sensitive quantification with a limit of detection (LOD) of 7.04 zM (1-2 molecules of AFP/GNI), which was 100-5000000000 times lower than detection limits obtained with previous AFP detection methods. Furthermore, the method was also successfully applied to quantify AFP molecules at the single-molecule level in human serum samples. The wavelength-dependent TIRS-SRM method was demonstrated to be an effective tool for superlocalizing individual protein molecules and interactions in nanoscale regions and was a reliable method for the ultrasensitive quantitative detection of disease-related protein molecules as a nanosensor and for diagnosis at the single-molecule level.

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Plasmonic metal scattering immunoassay by total internal reflection scattering microscopy with nanoscale lateral resolution

Cited by 12 publications

References 27 publications

Plasmon-Amplified Endogenous Fluorescence Nanospectroscopic Sensor Based on Inherent Elastic Scattering for Ultratrace Ratiometric Detection of Capsaicinoids

Plasmon-Amplified Endogenous Fluorescence Nanospectroscopic Sensor Based on Inherent Elastic Scattering for Ultratrace Ratiometric Detection of Capsaicinoids

In Situ Enzymatic Generation of Gold Nanoparticles for Nanozymatic Label-free Detection of Acid Phosphatase

Ultrasensitive Detection of α-Fetoprotein by Total Internal Reflection Scattering-Based Super-Resolution Microscopy for Superlocalization of Nano-Immunoplasmonics

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