Plasmodium falciparum protein phosphatase type 1 functionally complements a glc7 mutant in Saccharomyces cerevisiae

Regulation of protein phosphatase type 1 and cell cycle progression by PfLRR1, a novel leucine‐rich repeat protein of the human malaria parasite Plasmodium falciparum

“…1999; Garcia et al, 1999;Mamoun and Goldberg, 2001;Bhattacharyya et al, 2002;Kumar et al, 2002;. The importance of Sds22 in the regulation of yeast and mammalian PP1 activity prompted us to identify the P. falciparum orthologue and to address its function.…”

“…Enzymatic activities and genes related to plasmodial phosphatases (PPs) have been identified. These include PfPP1, PfPP2A, PfPP2B and PfPP2C (Li and Baker, 1997;1998;Mamoun et al ., 1998;Dobson et al ., 1999;Bhattacharyya et al ., 2002;Kumar et al ., 2002). The PfPP1 protein sequence (Bhattacharyya et al ., 2002) possesses the signature of Ser/Thr phosphatase LRGNHE, as well as two putative protein kinase C and five putative casein kinase II phosphorylation sites.…”

“…These include PfPP1, PfPP2A, PfPP2B and PfPP2C (Li and Baker, 1997;1998;Mamoun et al ., 1998;Dobson et al ., 1999;Bhattacharyya et al ., 2002;Kumar et al ., 2002). The PfPP1 protein sequence (Bhattacharyya et al ., 2002) possesses the signature of Ser/Thr phosphatase LRGNHE, as well as two putative protein kinase C and five putative casein kinase II phosphorylation sites. Further, the use of an inhibitor of protein phosphatase type 1 (PP1), okadaic acid (OA), suggested that PP1 activity predominates over PP2A activity in intraerythrocytic P. falciparum (Dobson et al ., 1999).…”

“…Further, the use of an inhibitor of protein phosphatase type 1 (PP1), okadaic acid (OA), suggested that PP1 activity predominates over PP2A activity in intraerythrocytic P. falciparum (Dobson et al ., 1999). Complementation studies showed that PfPP1 could rescue the low glycogen phenotype of Saccharomyces cerevisiae glc7 (PP1 -) mutant (Bhattacharyya et al ., 2002). Finally, phenotypic PfPP1 gene knockdown indicated its essential role in parasite asexual development in erythrocytes .…”

Functional expression of heterologous proteins in yeast: insights into Ca2+ signaling and Ca2+-transporting ATPases

“…In laboratories around the world, many types of genes from bacteria (56), protozoa (7,12,31), fungi (100), plants (48), and animals (83) have been expressed in and shown to complement deletion phenotypes of yeast mutants. The references given here are a very few examples of the vast number of articles published on the subject of functional complementation studies in yeast.…”