Functional expression of heterologous proteins in yeast: insights into Ca²⁺ signaling and Ca²⁺-transporting ATPases

Abstract

Ton, Van-Khue, and Rajini Rao. Functional expression of heterologous proteins in yeast: insights into Ca 2ϩ signaling and Ca 2ϩ -transporting ATPases.

Golgi Manganese Transport Is Required for Rapamycin Signaling in Saccharomyces cerevisiae

“…This P2-type Ca 21 ATPase restores growth of a pmr1D strain in EGTA-or BAPTA-containing media (Durr et al 1998;Degand et al 1999). In contrast, SERCA1 was unable to restore growth of pmr1D in high Mn 21 (Ton and Rao 2004). This suggests rapamycin resistance of pmr1D is not due to entry of Ca 21 into the secretory pathway.…”

Role of Four Calcium Transport Proteins, Encoded bynca-1,nca-2,nca-3, andcax, in Maintaining Intracellular Calcium Levels in Neurospora crassa

“…In higher eukaryotes, both plants and animals, three types of Ca 2ϩ -ATPases are involved in maintaining the proper level of calcium within cells (8,36,38). The PMCA type is localized primarily in the plasma membrane, where it removes excess calcium from the cytosol.…”

A Novel Isoform of the Secretory Pathway Ca2+,Mn2+-ATPase, hSPCA2, Has Unusual Properties and Is Expressed in the Brain

“…In contrast, a cDNA clone from human brain, KIAA0703, corresponding to a second SPCA isoform, was reported to have a more limited tissue distribution (18). Initial attempts to evaluate the function of this clone failed 2 and appeared to be due to an incorrect N-terminal sequence, presumably the result of a cloning artifact. We now report that the corrected clone encodes a functional SPCA pump that clearly differs from hSPCA1 in its ability to complement the phenotypes of a pmr1 null strain of yeast.…”