Plasmodium berghei: Gametocyte production, DNA content, and chromosome-size polymorphisms during asexual multiplication in vivo

Janse, Chris J.; Boorsma, E. G.; Ramesar, Jai; Vianen, P van; Meer, R.B. Van Der; Zenobi, P; Casaglia, O; Mons, Barend; Berg, Frida

doi:10.1016/0014-4894(89)90109-4

Cited by 63 publications

(29 citation statements)

References 29 publications

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“…Consistent with reported studies of rodent malaria karyotype (Sheppard et al, 1989 ;Sharkey et al, 1988 ;Langsley et al, 1987 andJanse et al, 1989) ; the present analysis shows an important karyotype polymorphism within and between species, strains and lines. Each cloned parasite exhibits an unique karyotype pattern which remains stable after mecha nical or cyclical passages (Ahamada Said, 1987).…”

Section: Number and Positions Of The Bandssupporting

confidence: 93%

“…Using PFG many studies have been carried out on the chromosomes number of plasmodia. These studies have shown that the chromosomes are highly polymorphic in size and that the number of chromosomes seems to be 14 (Kemp et al, 1985 ;Sharkey et al, 1988 ;Janse et al, 1989 ;Ponzi et al, 1990). Laboratories where several species or subspecies of rodent malaria parasites are simultaneously in use for different research studies need specific markers for detecting mixed infections.…”

Section: Introductionmentioning

confidence: 99%

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Rodent malaria parasites: molecular karyotypes characterize species, subspecies and lines

1994

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Summary :The molecular karyotypes of the African murine malaria parasites P. berghei (3 strains, 2 lines) P. yoelii (2 strains) P. chabaudi (3 strains, 1 line) and P. vinckei (4 strains) have been studied using orthogonal field alternation gel electrophoresis (OFAGE).The genome of each species was resolved into 9 to 11 distinct chromosomal DNA bands molecules of varying intensities which seem to represent 14 chromosomes ranging in size from 600 kb

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Section: Number and Positions Of The Bandssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Rodent malaria parasites: molecular karyotypes characterize species, subspecies and lines

1994

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“…4) also reveal an extensive restriction site polymorphism in subtelomeric regions among different pure populations of P. berghei which also exhibit extensive chromosome-size polymorphisms (11). The observed variation in restriction fragment length might be explained by assuming that the number of 2.3-kb units, present on a given chromosomal extremity after the last HaeIII restriction site, may vary to a large extent.…”

mentioning

confidence: 87%

Organization of subtelomeric repeats in Plasmodium berghei.

Dore¹,

Pace²,

Ponzi³

et al. 1990

Mol. Cell. Biol.

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Several (but not all) Plasmodium berghei chromosomes bear in the subtelomeric position a cluster of 2.3-kilobase (kb) tandem repeats. The 2.3-kb unit contains 160 base pairs of telomeric sequence. The resulting subtelomeric structure is one in which stretches of telomeric sequences are periodically spaced by a 2.1-kb reiterated sequence. This periodic organization of internal telomeric sequences might be related to chromosome-size polymorphisms involving the loss or addition of subtelomeric 2.3-kb units.

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“…Nonetheless, as previously shown, unlike P. berghei clone 2.34, clone 2.33 was incapable of infecting mosquitoes (11; data not shown). Several P. berghei laboratory strains have been shown to have irreversibly lost the ability to produce gametocytes and therefore are unable to infect mosquitoes (17,18). Although not conclusive, it was suggested that this defect may be associated with changes in the parasite's karyotype (17,18).…”

Section: Vol 74 2006mentioning

confidence: 99%

“…Several P. berghei laboratory strains have been shown to have irreversibly lost the ability to produce gametocytes and therefore are unable to infect mosquitoes (17,18). Although not conclusive, it was suggested that this defect may be associated with changes in the parasite's karyotype (17,18). However, the P. berghei ANKA clone 2.33 appears to produce gametocyte-like forms which could represent a form of incomplete or aborted sexual development since these gametocytes not only look abnormal but also are incapable of infecting mosquitoes.…”

Section: Vol 74 2006mentioning

confidence: 99%

Infection withPlasmodium bergheiBoosts Antibody Responses Primed by a DNA Vaccine Encoding Gametocyte Antigen Pbs48/45

2006

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An important consideration in the development of a malaria vaccine for individuals living in areas of endemicity is whether vaccine-elicited immune responses can be boosted by natural infection. To investigate this question, we used Plasmodium berghei ANKA blood-stage parasites for the infection of mice that were previously immunized with a DNA vaccine encoding the P. berghei sexual-stage antigen Pbs48/45. Intramuscular immunization in mice with one or two doses of DNA-Pbs48/45 or of empty DNA vaccine as control did not elicit detectable anti-Pbs48/45 antibodies as determined by enzyme-linked immunosorbent assay. An infection with P. berghei ANKA 6 weeks after DNA vaccination elicited comparable anti-Pbs48/45 antibody levels in mice which had been primed with DNA-Pbs48/45 or with empty DNA vaccine. However, a repeat infection with P. berghei ANKA resulted in significantly higher anti-Pbs48/45 antibody levels in mice which had been primed with the DNA-Pbs48/45 vaccine than the levels in the mock DNA-vaccinated mice. In parallel and as an additional control to distinguish the boosting of Pbs48/45 antibodies exclusively by gametocytes during infection, a separate group of mice primed with DNA-Pbs48/45 received an infection with P. berghei ANKA clone 2.33, which was previously described as a "nongametocyte producer." To our surprise, this parasite clone too elicited antibody levels comparable to those induced by the P. berghei gametocyte producer clone. We further demonstrate that the nongametocyte producer P. berghei clone is in fact a defective gametocyte producer that expresses Pbs48/45, much like the gametocyte producer clone, and is therefore capable of boosting antibody levels to Pbs48/45. Taken together, these results indicate that vaccine-primed antibodies can be boosted during repeat infections and warrant further investigation with additional malaria antigens.

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Plasmodium berghei: Gametocyte production, DNA content, and chromosome-size polymorphisms during asexual multiplication in vivo

Cited by 63 publications

References 29 publications

Rodent malaria parasites: molecular karyotypes characterize species, subspecies and lines

Rodent malaria parasites: molecular karyotypes characterize species, subspecies and lines

Organization of subtelomeric repeats in Plasmodium berghei.

Infection withPlasmodium bergheiBoosts Antibody Responses Primed by a DNA Vaccine Encoding Gametocyte Antigen Pbs48/45

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