Plasmodium berghei crystalloids contain multiple LCCL proteins

Saeed, Sadia; Carter, Victoria; Tremp, Annie Z.; Dessens, Johannes T.

doi:10.1016/j.molbiopara.2009.11.008

Cited by 46 publications

(67 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This plasmid contains lap3 upstream regulatory regions, allowing for the episomal expression of LAP3:: mCherry under its endogenous promoter. LAP3 has previously been localized to the ookinete crystalloids by both live fluorescence imaging and immunoelectron microscopy (27). DHHC10 perfectly colocalized with LAP3 in these experiments, unambiguously identifying this PAT in the ookinete crystalloid (Fig.…”

Section: Dhhc10supporting

confidence: 66%

“…2B and SI Appendix, Fig. S4) known for members of the LAP/ CCp protein family typical for the ookinete/oocyst-specific crystalloid organelle (8,26,27). Believed to act as a reservoir for proteins required for sporozoite development within the oocyst, these organelles are generated in ookinetes but dissolve in early oocysts and are absent in late oocysts (6,9).…”

Section: Dhhc10mentioning

confidence: 99%

“…To identify the consequences of crystalloid absence in Δdhhc10 ookinetes for crystalloid-resident proteins, we tagged LAP2 with GFP in the WT and Δdhhc10 null mutant genetic backgrounds (27). P. berghei LAP2 is a member of the LCCL multigene family of proteins expressed in female gametocytes with a typically scattered staining pattern in the WT background; during ookinete formation, it is concentrated in the crystalloid (27) (Fig.…”

Section: Dhhc10mentioning

confidence: 99%

“…P. berghei LAP2 is a member of the LCCL multigene family of proteins expressed in female gametocytes with a typically scattered staining pattern in the WT background; during ookinete formation, it is concentrated in the crystalloid (27) (Fig. 4A).…”

Section: Dhhc10mentioning

confidence: 99%

See 3 more Smart Citations

Maternally supplied S-acyl-transferase is required for crystalloid organelle formation and transmission of the malaria parasite

Santos

Duarte

Kehrer

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

Transmission of the malaria parasite from the mammalian host to the mosquito vector requires the formation of adequately adapted parasite forms and stage-specific organelles. Here we show that formation of the crystalloid-a unique and short-lived organelle of the Plasmodium ookinete and oocyst stage required for sporogonyis dependent on the precisely timed expression of the S-acyltransferase DHHC10. DHHC10, translationally repressed in female Plasmodium berghei gametocytes, is activated translationally during ookinete formation, where the protein is essential for the formation of the crystalloid, the correct targeting of crystalloid-resident protein LAP2, and malaria parasite transmission.T he malaria parasite is capable of infecting both the vertebrate host and mosquito vector. After a mosquito blood meal, sexual precursor cells rapidly differentiate into mature gametes. In the mosquito midgut, the gametes mate to form a zygote that develops further into the motile ookinete. After crossing the midgut epithelium and establishing a sessile oocyst, the ookinete gives rise to thousands of sporozoites capable of infecting a subsequent mammalian host (1).Sharing key organelles like the nucleus, endoplasmic reticulum, Golgi, and mitochondria with other eukaryotes, this parasite has evolved specialized, stage-specific structures that are necessary for developmental progression during parasite transmission. These include, for example, osmiophilic bodies (secretory vesicles) that release protein factors capable of lysing the parasitophorous vacuole and erythrocyte membranes, thus producing free gametes (2) and a gliding motility motor anchored to the inner membrane complex (IMC), allowing the ookinete to migrate across the mosquito midgut epithelium and establish an oocyst (3). Sporozoite formation in the oocyst finally requires the presence of a stage-specific organelle, the crystalloid, a multivesicular structure assembled in the ookinete and putative reservoir of proteins and lipids used during sporogony. Although this enigmatic organelle was discovered more than 40 y ago, its formation and function remain largely unknown (4-9). Six LCCL proteins have been shown to reside within (9) and maintain the stability (8, 9) of these organelles essential for sporogony (10).The morphological changes taking place during zygote-toookinete development and the generation of thousands of sporozoites inside a single oocyst require extensive protein translation and membrane biogenesis to support the formation of organelles and plasma membrane (PM) surrounding each new parasite. Onethird of the proteins identified in the oocyst and oocyst-derived (midgut) sporozoites of the human parasite Plasmodium falciparum are putatively membrane-bound (11). The targeting of such proteins to organelles, and perhaps formation of certain organelles per se, requires appropriate sorting signals, along with transmembrane (TM) domains to keep these factors in place. Posttranslational modifications, such as lipidation, can increase the affinity of a modif...

show abstract

Section: Dhhc10supporting

confidence: 66%

Section: Dhhc10mentioning

confidence: 99%

Section: Dhhc10mentioning

confidence: 99%

Section: Dhhc10mentioning

confidence: 99%

See 2 more Smart Citations

Maternally supplied S-acyl-transferase is required for crystalloid organelle formation and transmission of the malaria parasite

Santos

Duarte

Kehrer

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Using established transfection methodologies [24] we introduced a GFP tag into each locus, bodies, similar to that observed for the LCCL protein family in ookinete stages [45]. The LCCL and COWP protein families, along with the CPW-WPC family are conserved among pre-apicomplexan alveolates, and are likely to be markers for an archetypal preapicomplexan/ alveolate structure.…”

Section: Cpw-wpc Proteins Are Composed Of Arrays Of a Domain Conservementioning

confidence: 96%

Translational repression of the cpw-wpc gene family in the malaria parasite Plasmodium

Rao

Santos

Pain³

et al. 2016

Parasitology International

View full text Add to dashboard Cite

Please cite this article as: Rao Pavitra N., Santos Jorge M., Pain Arnab, Templeton Thomas J., Mair Gunnar R., Translational repression of the cpw-wpc gene family in the malaria parasite Plasmodium, Parasitology International (2016), doi: 10.1016/j.parint.2016 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Antimalarial Transmission-Blocking Vaccines

Sinden

Biswas

Blagborough

2017

Malaria

View full text Add to dashboard Cite

Plasmodium berghei crystalloids contain multiple LCCL proteins

Abstract: Graphical abstractThis paper describes the gametocyte-specific expression and subsequent targeting of two LCCL proteins to the crystalloids of Plasmodium berghei ookinetes using GFP-tagging.

Cited by 46 publications

References 20 publications

Maternally supplied S-acyl-transferase is required for crystalloid organelle formation and transmission of the malaria parasite

Maternally supplied S-acyl-transferase is required for crystalloid organelle formation and transmission of the malaria parasite

Translational repression of the cpw-wpc gene family in the malaria parasite Plasmodium

Antimalarial Transmission-Blocking Vaccines

Contact Info

Product

Resources

About