Plasminogen activator inhibitor (PAI)-1 suppresses inhibition of gastric emptying by cholecystokinin (CCK) in mice

Gamble, Joanne; Kenny, Susan; Dockray, Graham J.

doi:10.1016/j.regpep.2013.06.005

Cited by 12 publications

(5 citation statements)

References 33 publications

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“…In this study, we tested serum leptin concentrations in mesenteric vein and carotid artery and found that dietary lysine restriction tended to reduce serum leptin concentrations in mesenteric vein, indicating that lysine restriction associated with leptin abundance failed to inhibit hunger, which then enhanced feed intake in piglets. CKK also acts as a hunger suppressant and the mechanism for hunger suppression has been considered to be a decrease in the rate of gastric emptying [39]. In this study, we found that Lys restriction markedly downregulated intestinal CCK expression.…”

Section: Discussionsupporting

confidence: 51%

Lysine Restriction Affects Feed Intake and Amino Acid Metabolism via Gut Microbiome in Piglets

Yin

Han

et al. 2017

Cell Physiol Biochem

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Background/Aims: Our previous reports suggested that dietary supplementation with lysine influenced intestinal absorption and metabolism of amino acids. In this study, we further investigated the effect of lysine restriction (30%) on feed intake and we also tested the hypothesis that gut microbiome contributed to the potential mechanism of lysine restriction-mediated feeding behavior. Here, we profiled gut microbial communities by sequencing 16S ribosomal ribonucleic acid (rRNA) genes from gut samples as well as growth performance, serum hormones, and intestinal lysine transport in a piglet model. Results: Piglets preferred to the lysine restricted diet when giving three diets and the feed intake was markedly higher in the lysine-restricted group than that in the control group. Altered hormones (leptin, CCK, and ghrelin) might contribute to the feeding behavior caused by lysine restriction. Meanwhile, lysine transporting ability (SLC7A1 and SLC7A2 expression, intestinal electrophysiological changes, and amino acid pool in mesenteric vein) was decreased in response to lysine restriction. Through deep sequencing of bacterial rRNA markers, we observed that bacterial diversity was enhanced in the lysine-restricted group (Shannon H, PD, and Chao1). At the phylum level, lysine restriction enhanced gut Actinobacteria, Saccharibacteria, and Synergistetes abundances. At the family level, Moraxellaceae, Halomonadaceae, Shewanellaceae, Corynebacteriaceae, Bacillaceae, Comamonadaceae, Microbacteriaceae, Caulobacteraceae, and Synergistaceae abundances were increased in response to lysine restriction. Predictive functional profiling of microbial communities by PICRUSt also confirmed that dietary lysine restriction affected gut microbiome, which might further mediate amino acid metabolism, membrane transport, and endocrine system. Conclusion: Our results indicated that lysine restriction inhibited intestinal lysine transport and promoted feed intake, which might be associated with gut microbiome.

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Section: Discussionsupporting

confidence: 51%

Lysine Restriction Affects Feed Intake and Amino Acid Metabolism via Gut Microbiome in Piglets

Yin

Han

et al. 2017

Cell Physiol Biochem

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“…CCK mainly regulates feeding behavior by stimulating the vagal nerve through the CCK receptor (CCKAR) (Cookand, 2004) and then terminates feeding behavior while promoting metabolism (Pekas and Trout, 1990). Oral treatment with anti-CCK antibodies improves feed efficiency and the growth rate in broilers (Cawthon and Serre, 2021), and immunization of piglets with CCK can dramatically increase feed intake and growth rate (Gamble et al, 2013). One feature of diet-induced obesity (DIO) in humans has reduced sensitivity to CCK, and vagal afferent neurons phenotypic flexibility is lost in DIO (Mei and Zhu, 2015).…”

Section: Discussionmentioning

confidence: 99%

Characterization and Duodenal Transcriptome Analysis of Chinese Beef Cattle With Divergent Feed Efficiency Using RNA-Seq

Yang

Han

et al. 2021

Front. Genet.

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Residual feed intake (RFI) is an important measure of feed efficiency for agricultural animals. Factors associated with cattle RFI include physiology, dietary factors, and the environment. However, a precise genetic mechanism underlying cattle RFI variations in duodenal tissue is currently unavailable. The present study aimed to identify the key genes and functional pathways contributing to variance in cattle RFI phenotypes using RNA sequencing (RNA-seq). Six bulls with extremely high or low RFIs were selected for detecting differentially expressed genes (DEGs) by RNA-seq, followed by conducting GO, KEGG enrichment, protein-protein interaction (PPI), and co-expression network (WGCNA, n = 10) analysis. A total of 380 differentially expressed genes was obtained from high and low RFI groups, including genes related to energy metabolism (ALDOA, HADHB, INPPL1), mitochondrial function (NDUFS1, RFN4, CUL1), and feed intake behavior (CCK). Two key sub-networks and 26 key genes were detected using GO analysis of DEGs and PPI analysis, such as TPM1 and TPM2, which are involved in mitochondrial pathways and protein synthesis. Through WGCNA, a gene network was built, and genes were sorted into 27 modules, among which the blue (r = 0.72, p = 0.03) and salmon modules (r = −0.87, p = 0.002) were most closely related with RFI. DEGs and genes from the main sub-networks and closely related modules were largely involved in metabolism; oxidative phosphorylation; glucagon, ribosome, and N-glycan biosynthesis, and the MAPK and PI3K-Akt signaling pathways. Through WGCNA, five key genes, including FN1 and TPM2, associated with the biological regulation of oxidative processes and skeletal muscle development were identified. Taken together, our data suggest that the duodenum has specific biological functions in regulating feed intake. Our findings provide broad-scale perspectives for identifying potential pathways and key genes involved in the regulation of feed efficiency in beef cattle.

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“…PAI is an inflammatory marker, which is induced by pro-inflammatory cytokines [65] and affects gastric emptying and food intake and may induce hyperphagia [66,67]. As such, the positive associations of PAI-1 with MBDI may be explained by the inflammatory response during menstruation which is accompanied by increased PAI-1 regulating fibrinolysis.…”

Section: Discussionmentioning

confidence: 99%

Menstruation Distress Is Strongly Associated with Hormone-Immune-Metabolic Biomarkers

Roomruangwong¹,

Sirivichayakul²,

Matsumoto³

et al. 2020

Preprint

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Objective: To examine the associations between menstruation features and symptoms and hormone-immune-metabolic biomarkers. Methods: Forty-one women completed questionnaires assessing characteristic menstruation symptoms, duration of menstrual cycle and number of pads used/day and completed the Daily Record of Severity of Problems (DRSP) during the consecutive days of their menstrual cycle. Menses-related symptoms (MsRS) were computed from the sum of 10 pre- and post-menses symptoms and the menstruation blood and duration index (MBDI) was computed based on the daily number of pads and duration of menses. We assayed serum levels of various biomarkers at days 7, 14, 21, and 28 of the subjects’ menstrual cycle. Results: MBDI was significantly associated with a) MsRS including low abdominal cramps, and gastro-intestinal (GI) and pain symptoms (positively); b) plasma levels of haptoglobin (Hp), CCL5, insulin growth factor (IGF)-1, and plasminogen activator inhibitor (PAI)1 (all positively); and c) estradiol and paraoxonase (PON)1 arylesterase activity (both inversely). MsRS were significantly predicted by CCL5 and IGF-1 (both positively) and progesterone (inversely). Low-abdominal cramps, and gastro-intestinal and pain symptoms were associated with lower progesterone levels. The MBDI+MsRS score was significantly predicted by the cumulative effects of (in descending order of importance): Hp, IGF-1, PON1 arylesterase, estradiol and PAI. Conclusion: Menstruation-related features including estimated blood loss, duration of menses, cramps, pain and GI symptoms are associated with hormone-immune-metabolic biomarkers, which mechanistically may explain those features. Women with an increased MBDI+MsRS index ≥ 0.666 percentile may be considered to have menstruation-related distress, including dysmenorrhea symptoms.

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Plasminogen activator inhibitor (PAI)-1 suppresses inhibition of gastric emptying by cholecystokinin (CCK) in mice

Cited by 12 publications

References 33 publications

Lysine Restriction Affects Feed Intake and Amino Acid Metabolism via Gut Microbiome in Piglets

Lysine Restriction Affects Feed Intake and Amino Acid Metabolism via Gut Microbiome in Piglets

Characterization and Duodenal Transcriptome Analysis of Chinese Beef Cattle With Divergent Feed Efficiency Using RNA-Seq

Menstruation Distress Is Strongly Associated with Hormone-Immune-Metabolic Biomarkers

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