Plasmids of Pseudomonas cepacia strains of diverse origins

Lennon, E; DeCicco, B. T.

doi:10.1128/aem.57.8.2345-2350.1991

Cited by 22 publications

(7 citation statements)

References 22 publications

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“…Plasmids in excess of 100 kbp were harboured by both clinical and environmental isolates. Lennon & DeCicco (1991) suggested that large plasmids were associated with clinical isolates and high levels of antibiotic resistance but in this study, there was no evidence of large plasmids being more frequently harboured in CF isolates; indeed, many of the largest plasmids were found in environmental isolates. Strains which appeared identical by ribotyping and genomic fingerprinting were nevertheless found to differ in their plasmid content, e.g.…”

Section: Discussioncontrasting

confidence: 64%

Genotypic and phenotypic relationships in Burkholderia cepacia isolated from cystic fibrosis patients and the environment

Wigley¹,

Burton²

1999

J Appl Microbiol

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Twenty‐one strains of Burkholderia cepacia isolated from the environment, and 21 clinical strains isolated principally from sputum of cystic fibrosis (CF) patients, were characterized genotypically by macrorestriction analysis (genome fingerprinting) and PCR ribotyping, and phenotypically by susceptibility to antibiotics and the ability to macerate onion tissue. The plasmid content of the strains was also investigated. Environmental isolates showed a high degree of genetic variability, all strains differing from both one another and the CF isolates. The CF isolates were less variable, with common strains found in patients attending three geographically distinct CF centres. Phenotypic variation was found both within and between CF and environmental strains. Generally, CF isolates displayed higher levels of antibiotic resistance, while the ability to macerate onion tissue was more prevalent amongst environmental isolates. Plasmids were more frequently found in CF isolates, but were of similar size in both groups of strains. Such variability is not surprising in view of the existence of multiple genomovars within the B. cepacia complex.

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Section: Discussioncontrasting

confidence: 64%

Genotypic and phenotypic relationships in Burkholderia cepacia isolated from cystic fibrosis patients and the environment

Wigley¹,

Burton²

1999

J Appl Microbiol

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“…In fact, B. cepacia has an unusual genomic organization, characterized by the presence of multiple chromosomes regulated by separate control systems and by an extensive array of insertion sequences, which may play a fundamental role in its ability to adapt to different environments by genetic transfer and mutation (Lessie et al, 1996). The B. cepacia species is also characterized by an extraordinary nutritional versatility that favours the ability to colonize highly different habitats, among which are soil and plant rhizosphere (Lennon and DeCicco, 1991;Bevivino et al, 1994). In particular, B. cepacia is closely associated with maize roots, representing over 4% of the total culturable rhizobacteria (Hebbar et al, 1994;Di Cello et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Different portions of the maize root system host Burkholderia cepacia populations with different degrees of genetic polymorphism

Chiarini

Giovannelli

Bevivino

et al. 2000

Environmental Microbiology

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In order to acquire a better understanding of the spatial and temporal variations of genetic diversity of Burkholderia cepacia populations in the rhizosphere of Zea mays, 161 strains were isolated from three portions of the maize root system at different soil depths and at three distinct plant growth stages. The genetic diversity among B. cepacia isolates was analysed by means of the random amplified polymorphic DNA (RAPD) technique. A number of diversity indices (richness, Shannon diversity, evenness and mean genetic distance) were calculated for each bacterial population isolated from the different root system portions. Moreover, the analysis of molecular variance (AMOVA) method was applied to estimate the genetic differences among the various bacterial populations. Our results showed that, in young plants, B. cepacia colonized preferentially the upper part of the root system, whereas in mature plants, B. cepacia was mostly recovered from the terminal part of the root system. This uneven distribution of B. cepacia cells among different root system portions partially reflected marked genetic differences among the B. cepacia populations isolated along maize roots on three distinct sampling occasions. In fact, all the diversity indices calculated indicated that genetic diversity increased during plant development and that the highest diversity values were found in mature maize plants, in particular in the middle and terminal portions of the root system. Moreover, the analysis of RAPD patterns by means of the AMOVA method revealed highly significant divergences in the degree of genetic polymorphism among the various B. cepacia populations.

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“…As a species, B. cepacia has frequently been shown to contain a single plasmid or multiple plasmids of greater than 200 kb (19). Other studies have reported the presence of plasmids in up to 94% of the B. cepacia strains surveyed (9,22).…”

Section: Discussionmentioning

confidence: 99%

TOM, a new aromatic degradative plasmid from Burkholderia (Pseudomonas) cepacia G4

Shields

Reagin

Gerger

et al. 1995

Appl Environ Microbiol

128

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Burkholderia (Pseudomonas) cepacia PR1 23 has been shown to constitutively express a toluene catabolic pathway distinguished by a unique toluene ortho-monooxygenase (Tom). This strain has also been shown to contain two extrachromosomal elements of <70 and >100 kb. A derivative strain cured of the largest plasmid, PR1 23 Cure, was unable to grow on phenol or toluene as the sole source of carbon and energy, which requires expression of the Tom pathway. Transfer of the larger plasmid from strain G4 (the parent strain inducible for Tom) enabled PR1 23 Cure to grow on toluene or phenol via inducible Tom pathway expression. Conjugal transfer of TOM 23c from PR1 23 to an antibiotic-resistant derivative of PR1 23 Cure enabled the transconjugant to grow with either phenol or toluene as the sole source of carbon and energy through constitutive expression of the Tom pathway. A cloned 11.2-kb EcoRI restriction fragment of TOM 23c resulted in the expression of both Tom and catechol 2,3-dioxygenase in Escherichia coli, as evidenced by its ability to oxidize trichloroethylene, toluene, m-cresol, o-cresol, phenol, and catechol. The largest resident plasmid of PR1 was identified as the source of these genes by DNA hybridization. These results indicate that the genes which encode Tom and catechol 2,3-dioxygenase are located on TOM, an approximately 108-kb degradative plasmid of B. cepacia G4.

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Plasmids of Pseudomonas cepacia strains of diverse origins

Cited by 22 publications

References 22 publications

Genotypic and phenotypic relationships in Burkholderia cepacia isolated from cystic fibrosis patients and the environment

Genotypic and phenotypic relationships in Burkholderia cepacia isolated from cystic fibrosis patients and the environment

Different portions of the maize root system host Burkholderia cepacia populations with different degrees of genetic polymorphism

TOM, a new aromatic degradative plasmid from Burkholderia (Pseudomonas) cepacia G4

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