Different portions of the maize root system host <i>Burkholderia cepacia</i> populations with different degrees of genetic polymorphism

Chiarini, Luigi; Giovannelli, Valeria; Bevivino, Annamaria; Dalmastri, Claudia; Tabacchioni, Silvia

doi:10.1046/j.1462-2920.2000.00083.x

Cited by 19 publications

(12 citation statements)

References 21 publications

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“…So far, the genetic diversity of natural populations of 'B. cepacia' has been investigated only at the complex level [25,27,42] ; as a consequence, nothing is known about the genetic polymorphism of the Bcc species present in natural populations. In this study, all species and genomovars proved to be highly diverse; in fact, the E index based on RAPD types ranged from 0.98 to 1, indicating that almost all isolates were genetically unrelated.…”

Section: Discussionmentioning

confidence: 99%

A rhizospheric Burkholderia cepacia complex population: genotypic and phenotypic diversity of Burkholderia cenocepacia and Burkholderia ambifaria

Dalmastri

Fiore

Alisi

et al. 2003

FEMS Microbiology Ecology

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The Burkholderia cepacia‘complex’ (Bcc) presently comprises nine species and genomovars. In order to acquire a better comprehension of the species and genomovar distribution and of the genetic diversity among environmental Bcc bacteria, a natural population of 60 bacterial isolates recovered from the rhizosphere of maize and belonging to the Bcc has been characterised to assess the exact taxonomic position, the genetic polymorphism and the metabolic profiles of isolates. The identification of the different species and genomovars was accomplished by a combination of techniques including sodium dodecyl sulfate–polyacrylamide gel electrophoresis of whole‐cell proteins and recA‐based restriction fragment length polymorphism analyses. The genetic diversity among Bcc isolates was analysed by means of the random amplified polymorphic DNA and amplified fragment length polymorphism techniques; the analysis of molecular variance method was applied to estimate the genetic differences among the various species and genomovars identified within the bacterial population. Metabolic profiles based on carbon source utilisation were obtained by means of the Biolog GN assay and analysed by means of cluster analysis. Forty‐four strains were identified as B. ambifaria, 11 as B. cenocepacia recA lineage III‐B, four as B. pyrrocinia, and one as B. cepacia genomovar I. Marked genetic differences were observed between B. cenocepacia and B. ambifaria, whereas limited differences were found between B. pyrrocinia and B. ambifaria and between B. pyrrocinia and B. cenocepacia. No significant differences (P>0.05) were observed between the mean genetic distances of isolates belonging to B. cenocepacia, B. ambifaria, and B. pyrrocinia. Phenotypic analyses revealed that all isolates tested were able to utilise more than 75% of substrates. The highest variability in the number of utilised substrates was found among B. cenocepacia isolates, whereas the lowest was found among B. ambifaria isolates. Cluster analysis of metabolic profiles revealed pronounced differences between B. cenocepacia and B. ambifaria; in contrast, B. pyrrocinia could not be clearly separated either from B. cenocepacia or from B. ambifaria.

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Section: Discussionmentioning

confidence: 99%

A rhizospheric Burkholderia cepacia complex population: genotypic and phenotypic diversity of Burkholderia cenocepacia and Burkholderia ambifaria

Dalmastri

Fiore

Alisi

et al. 2003

FEMS Microbiology Ecology

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“…It is well known that bacteria of the B. cepacia complex (Bcc), which includes nine species or genomovars (26), may be found in soils (including polluted soils), the rhizospheres of crop plants, and water, as well as in various animal species, in humans, and in the hospital environment (13). Studies published in Europe have shown that Bcc bacteria are good colonizers of the maize rhizosphere, and they may represent one of the predominant bacterial groups in maize grown in Italy (10,17,19). In addition, the species B. graminis (48) and B. cepacia genomovar III (2), presently formally classified as B. cenocepacia (46), were shown to be abundant in maize fields analyzed in France.…”

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Diazotrophic Burkholderia Species Associated with Field-Grown Maize and Sugarcane

Perin

Martínez‐Aguilar

Castro-González

et al. 2006

Appl Environ Microbiol

125

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Until recently, diazotrophy was known in only one of the 30 formally described species of Burkholderia. Novel N 2 -fixing plant-associated Burkholderia species such as B. unamae, B. tropica, and B. xenovorans have been described, but their environmental distribution is scarcely known. In the present study, the occurrence of N 2 -fixing Burkholderia species associated with different varieties of sugarcane and maize growing in regions of Mexico and Brazil was analyzed. Only 111 out of more than 900 isolates recovered had N 2 -fixing ability as demonstrated by the acetylene reduction assay. All 111 isolates also yielded a PCR product with primers targeting the nifH gene, which encodes a key enzyme in the process of nitrogen fixation. These 111 isolates were confirmed as belonging to the genus Burkholderia by using a new 16S rRNA-specific primer pair for diazotrophic species (except B. vietnamiensis) and closely related nondiazotrophic Burkholderia. In Mexico, many isolates of B. unamae (predominantly associated with sugarcane) and B. tropica (more often associated with maize) were recovered. However, in Brazil B. tropica was not identified among the isolates analyzed, and only a few B. unamae isolates were recovered from one sugarcane variety. Most Brazilian diazotrophic Burkholderia isolates (associated with both sugarcane and maize plants) belonged to a novel species, as revealed by amplified 16S rRNA gene restriction profiles, 16S rRNA gene sequencing, and protein electrophoresis. In addition, transmissibility factors such as the cblA and esmR genes, identified among clinical and environmental isolates of opportunistic pathogens of B. cenocepacia and other species of the B. cepacia complex, were not detected in any of the plant-associated diazotrophic Burkholderia isolates analyzed.

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“…Despite the great agronomic potential of Burkholderia spp., there is general concern about the environment functioning as a source of human-pathogenic organisms, mainly after B. cenocepacia (genomovar III), which is associated with cepacia syndrome in cystic fibrosis patients, was found as a common plant-associated bacterium (3). Although this finding highlighted the importance of assessing the diversity of Burkholderia species in the rhizosphere, most current ecological knowledge is based on B. cepacia populations isolated from the rhizosphere of just one plant species, maize (5,10,13). Recently, the diversity of the Burkholderia community associated with woodland rhizospheres was also assessed (27).…”

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Multivariate Analyses of Burkholderia Species in Soil: Effect of Crop and Land Use History

Salles

Veen

Elsas

2004

Appl Environ Microbiol

154

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The assessment of Burkholderia diversity in agricultural areas is important considering the potential use of this genus for agronomic and environmental applications. Therefore, the aim of this work was to ascertain how plant species and land use management drive the diversity of the genus Burkholderia. In a greenhouse experiment, different crops, i.e., maize, oat, barley, and grass, were planted in pots containing soils with different land use histories, i.e., maize monoculture, crop rotation, and permanent grassland, for three consecutive growth cycles. The diversity of Burkholderia spp. in the rhizosphere soil was assessed by genus-specific PCR-denaturing gradient gel electrophoresis (DGGE) and analyzed by canonical correspondence analysis (CCA). CCA ordination plots showed that previous land use was the main factor affecting the composition of the Burkholderia community. Although most variation in the Burkholderia community structure was observed between the permanent grassland and agricultural areas, differences between the crop rotation and maize monoculture groups were also observed. Plant species affected Burkholderia community structure to a lesser extent than did land use history. Similarities were observed between Burkholderia populations associated with maize and grass, on the one hand, and between those associated with barley and oat, on the other hand. Additionally, CCA ordination plots demonstrated that these two groups (maize/grass versus barley/oat) had a negative correlation. The identification of bands from the DGGE patterns demonstrated that the species correlated with the environmental variables were mainly affiliated with Burkholderia species that are commonly isolated from soil, in particular Burkholderia glathei, B. caledonica, B. hospita, and B. caribiensis.

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Different portions of the maize root system host Burkholderia cepacia populations with different degrees of genetic polymorphism

Cited by 19 publications

References 21 publications

A rhizospheric Burkholderia cepacia complex population: genotypic and phenotypic diversity of Burkholderia cenocepacia and Burkholderia ambifaria

A rhizospheric Burkholderia cepacia complex population: genotypic and phenotypic diversity of Burkholderia cenocepacia and Burkholderia ambifaria

Diazotrophic Burkholderia Species Associated with Field-Grown Maize and Sugarcane

Multivariate Analyses of Burkholderia Species in Soil: Effect of Crop and Land Use History

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