Plasmid transfer to indigenous marine bacterial populations by natural transformation

Frischer, Marc E.; Stewart, Gregory J.; Paul, John H.

doi:10.1111/j.1574-6941.1994.tb00237.x

Cited by 46 publications

(17 citation statements)

References 29 publications

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“…Viruses could enhance genetic transfer directly, via transduction, or indirectly by contributing to the dissolved DNA pool, thus increasing the likelihood of natural transformation. Both processes have been shown to occur in aquatic environments (Saye et al 1990, Frischer et al 1994. The genes of bacterial viruses themselves may prove important determinants of bacterial phenotype through phage conversion.…”

Section: Bacterial Mortalitymentioning

confidence: 99%

Abundance and production of bacteria and viruses in the Bering and Chukchi Seas

Steward¹,

Smith²,

Azam³

1996

Mar. Ecol. Prog. Ser.

284

264

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The distribution, abundance, and production of viruses and bactena were investigated during an August to September 1992 cruise aboard the RV 'Alpha Helix' in the Bering and Chukchi Seas. Viruses were abundant in seawater samples at all stations (10' to 10'' I-') and exceeded the bacteria concentration by an order of magnitude on average. Virus-like particles and bacteria were also observed in the pore water of a sed~ment sample at 27 and 2.1 X 10' l.', respectively. The concentrations of viruses and bacteria in pelagic samples were correlated (r = 0.83, n = 43). In a detailed depth profile from the deepest and northernmost station (72' N), bacteria and viruses displayed subsurface maxima in the upper 100 m. Below 100 m, the concentrations declined, but were detectable even in the deepestcollected samples (402 m). Integrated bacterial biomass estimates were similar to results from a previous study in this area, but bacterial production measurements ranging from 0.3 to 0.45 g C m-2 d-' were an order of magnitude higher Production rates of bacterial viruses (also known as bacteriophages or simply phages) measured by radiolabeling ranged from 0.5 to 4.2 X lO%iruses I-' d-l, which are similar to previous estimates for temperate coastal waters. The production measurements ind~cated turnover times ranging from 0.4 to 17 d for bacteria and maximum estimates of 1.2 to 15 d for bacterial viruses. Viral mortality of bactena was estimated from the frequency of visibly infected cells (FVIC) and flagellate grazing was calculated from flagellate and bacterial abundances together with an assumed flagellate clearance rate. Overall, estimated viral lysis was roughly comparable to estimated grazing by flagellates as a source of bacterial mortality. Averaged over the water column, viral mortality of bacteria in the Chukchi Sea was estimated to be 23% of the bacterial production at 2 southern stations and approximately 10% at 2 northern stations. FVIC was correlated with bacterial production (r = 0.75, n = 18) and specific growth rate (r = 0.74, n = 18), but not with bacterial abundance (r = 0.22, n = 27). These data suggest viruses to be a ubiquitous and dynamic feature and a significant source of bacterial mortality in Arct~c marine microbial communities. The implications of bacterial and viral production for C and N cycling in the Chukchi Sea are discussed

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Section: Bacterial Mortalitymentioning

confidence: 99%

Abundance and production of bacteria and viruses in the Bering and Chukchi Seas

Steward¹,

Smith²,

Azam³

1996

Mar. Ecol. Prog. Ser.

284

264

View full text Add to dashboard Cite

show abstract

“…For example, extensive gene sharing has been observed throughout the commensal human microbiome, including sharing of genes that enable nutrient acquisition from novel food sources (Hehemann et al, 2010;Smillie et al, 2011), and pathogenicity islands and antibiotic resistance genes in pathogenic microbes Hiramatsu et al, 2001;Forsberg et al, 2012). There is evidence of extensive HGT in other natural habitats, such as soil (Coombs and Barkay, 2004;Heuer and Smalla, 2012) and aquatic environments (McDaniel et al, 2010;Frischer et al, 1994). Although these studies offer valuable insights into the frequency and potential impact of genes that can be transferred in microbial communities, the complexity of these systems makes difficult any further examination of the effects of these HGT events on their evolution and ecology.…”

Section: Introductionmentioning

confidence: 99%

Extensive Horizontal Gene Transfer in Cheese-Associated Bacteria

Bonham

Wolfe

Dutton

2016

Preprint

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Acquisition of genes through horizontal gene transfer (HGT) allows microbes to rapidly gain new capabilities and adapt to new or changing environments. Identifying widespread HGT regions within multispecies microbiomes can pinpoint the molecular mechanisms that play key roles in microbiome assembly. We sought to identify horizontally transferred genes within a model microbiome, the cheese rind. Comparing 31 newly sequenced and 134 previously sequenced bacterial isolates from cheese rinds, we identified over 200 putative horizontally transferred genomic regions containing 4733 protein coding genes. The largest of these regions are enriched for genes involved in siderophore acquisition, and are widely distributed in cheese rinds in both Europe and the US. These results suggest that HGT is prevalent in cheese rind microbiomes, and that identification of genes that are frequently transferred in a particular environment may provide insight into the selective forces shaping microbial communities.

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“…Some species are naturally competent, and some species require manipulation to become competent (11). Ten percent of marine isolates have been reported to be transformable by plasmids, and 14% are transformable by chromosome fragments (13). One study suggested that Pseudomonas fluorescens is transformable in its native soil environment but not in vitro (10).…”

mentioning

confidence: 99%

Quantitative Determination of Free-DNA Uptake in River Bacteria at the Single-Cell Level by In Situ Rolling-Circle Amplification

Maruyama

Tani

Kenzaka

et al. 2006

Appl Environ Microbiol

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Detection of plasmid DNA uptake in river bacteria at the single-cell level was carried out by rolling-circle amplification (RCA). Uptake of a plasmid containing the green fluorescent protein gene (gfp) by indigenous bacteria from two rivers in Osaka, Japan, was monitored for 506 h using this in situ gene amplification technique with optimized cell permeabilization conditions. Plasmid uptake determined by in situ RCA was compared to direct counts of cells expressing gfp under fluorescence microscopy to examine differences in detection sensitivities between the two methods. Detection of DNA uptake as monitored by in situ RCA was 20 times higher at maximum than that by direct counting of gfp-expressing cells. In situ RCA could detect bacteria taking up the plasmid in several samples in which no gfp-expressing cells were apparent, indicating that in situ gene amplification techniques can be used to determine accurate rates of extracellular DNA uptake by indigenous bacteria in aquatic environments.

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Plasmid transfer to indigenous marine bacterial populations by natural transformation

Cited by 46 publications

References 29 publications

Abundance and production of bacteria and viruses in the Bering and Chukchi Seas

Abundance and production of bacteria and viruses in the Bering and Chukchi Seas

Extensive Horizontal Gene Transfer in Cheese-Associated Bacteria

Quantitative Determination of Free-DNA Uptake in River Bacteria at the Single-Cell Level by In Situ Rolling-Circle Amplification

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