Plasmid-related Resistance to Clindamycin and Penicillin G in a Bacteroides vulgatus Strain

Pestana, Ana Cristina Nogueira Rodrigues; Diniz, Cláudio Galuppo; Farias, Luiz M.; Carvalho, Maria Auxiliadora Roque de

doi:10.1006/anae.1999.0281

Cited by 8 publications

(6 citation statements)

References 5 publications

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“…In Brazil, Avila-Campos et al (1991), Carvalho et al (1997), and Pestana et al (1999) have analyzed the antimicrobial susceptibility of B. fragilis isolated from both human and Callithrix penicillata marmoset gastrointestinal tracts showing an increase of resistance to drugs. Bacteria of the B. fragilis group have shown an interesting multiple resistance to metronidazole, imipenem, and amoxycillin/clavulanic acid (Turner et al 1995).…”

Section: Discussionmentioning

confidence: 99%

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Virulence markers and antimicrobial susceptibility of bacteria of the Bacteroides fragilis group isolated from stool of children with diarrhea in São Paulo, Brazil

Nakano

Ávila-Campos

2004

Mem. Inst. Oswaldo Cruz

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Section: Discussionmentioning

confidence: 99%

“…Pestana et al (1999) showed that from 5 B. fragilis strains, only 1 strain harbored plasmid, which was associated to the resistance to penicillin and clindamycin. However, several studies have shown that Bacteroides spp.…”

Section: Discussionmentioning

confidence: 99%

Virulence markers and antimicrobial susceptibility of bacteria of the Bacteroides fragilis group isolated from stool of children with diarrhea in São Paulo, Brazil

Nakano

Ávila-Campos

2004

Mem. Inst. Oswaldo Cruz

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“…Another report suggests that loss of 6.4-and 3.8-kb plasmids in Bacteroides fragilis C68c was related to antibiotic resistance (Nakano et al, 2004). According to Pestana et al (1999), the absence of plasmid suggests that the resistances are chromosomally mediated. Similarly, Radu et al (2003), analyzing Aeromonas spp.…”

Section: Streptococcus Micrococcusmentioning

confidence: 95%

Study on acquisition of bacterial antibiotic resistance determinants in poultry litter

et al. 2009

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Antibiotic resistance and the mode of transmission were investigated in bacteria isolated from poultry litter. Total aerobic heterotrophic bacteria were screened and identified for their resistance to different antibiotics such as ampicillin, streptomycin, erythromycin, tetracycline, chloramphenicol, kanamycin, tobramycin, and rifampicin. The distribution of bacteria found in the litter was Staphylococcus (29.1%), which was the predominant group, followed by Streptococcus (25%), Micrococcus (20.8%), Escherichia coli (12.5%), Salmonella (8.3%), and Aeromonas (4.1%). Fifty percent of these isolates were susceptible to ampicillin, 57% to erythromycin, 25% to tetracycline, 4% to chloramphenicol, 40% to kanamycin, 75% to streptomycin, 54% to tobramycin, and 4% to rifampicin. Three randomly selected isolates representing Staphylococcus, Streptococcus, and Micrococcus were examined for plasmids, and plasmid-curing and plasmid-induced transformation studies were conducted. Streptococcus and Micrococcus harbored a plasmid of 4.2 and 5.1 kb, respectively, whereas Staphylococcus did not harbor any plasmids. Plasmids were cured in Streptococcus and Micrococcus at a concentration of 75 and 100 microg/ mL of acridine orange, respectively, and transformation of 4.2- and 5.1-kb plasmids isolated from the Streptococcus and Micrococcus to plasmid-free E. coli DH5alpha strain was possible. In conjugation experiments, the antibiotic resistance profiles of transconjugant cells were found to be the same as the donors with the exception of Staphylococcus. The results of this study suggest that transformation and conjugation could be an important mechanism for horizontal gene transfer between bacteria in poultry litter. An understanding of the mechanism and magnitude of resistance gene transfer may provide a strategy to reduce the potential for dissemination of these genes.

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“…However, most of the bacteria showed high levels of resistance to most of the antimicrobials tested, and these results may indicate that animals received drugs with food or from soil contaminated with, for example, metal ions. Brazilian studies have reported the antimicrobial susceptibility of species of the B. fragilis group isolated from both human and Callithrix penicillata marmoset gastrointestinal tracts showing an increase of resistance to several drugs (Avila‐Campos et al , 1991; Carvalho et al , 1997; Pestana et al , 1999). The high frequency of isolation and the predominance of B. fragilis in calves could also be attributed to variable breeding conditions, such as possible outside runs, nonauthorized use of antimicrobial growth promoters, limited use of antimicrobials for therapy and age at slaughter.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of enterotoxigenic and nonenterotoxigenicBacteroides fragilisin calves and evaluation of their antimicrobial susceptibility

Almeida¹,

Nakano²,

Avila-Campos³

2007

FEMS Microbiology Letters

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Bacteroides fragilis is considered an important clinical pathogen and the most common anaerobe isolated from human and animal clinical specimens; enterotoxigenic strains produce diarrhea. The presence of enterotoxigenic (ETBF) and nonenterotoxigenic B. fragilis in stool samples from calves with or without acute diarrhea and the antimicrobial susceptibility of the strains were evaluated. The stool samples were plated onto a selective B. fragilis-bile-esculin agar, and incubated anaerobically (10% CO(2)/90% N(2)), at 37 degrees C, for 72 h. Species of the B. fragilis group were identified by using the API 32-A kit. Enterotoxigenic strains were detected by PCR and the cytotoxic assay. From 54 diarrhea and 54 nondiarrhea stools, 124 and 92 members of the B. fragilis group, respectively, were recovered. Only two ETBF strains were isolated from two different diarrhea samples and the bft gene was detected in both. Moreover, the bft gene was detected in DNA from four different diarrheal stools samples but no ETBF strain was recovered. All the bacteria were susceptible to chloramphenicol, imipenem, moxifloxacin, piperacillin/tazobactam, metronidazole and tigecycline. Most of the isolates from both calves with and without diarrhea were resistant to all metals. Our results are of concern, and suggest the need to increase the surveillance of antibiotic and metal resistance of this microbial group isolated from animal production such as calves.

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Plasmid-related Resistance to Clindamycin and Penicillin G in a Bacteroides vulgatus Strain

Cited by 8 publications

References 5 publications

Virulence markers and antimicrobial susceptibility of bacteria of the Bacteroides fragilis group isolated from stool of children with diarrhea in São Paulo, Brazil

Virulence markers and antimicrobial susceptibility of bacteria of the Bacteroides fragilis group isolated from stool of children with diarrhea in São Paulo, Brazil

Study on acquisition of bacterial antibiotic resistance determinants in poultry litter

Occurrence of enterotoxigenic and nonenterotoxigenicBacteroides fragilisin calves and evaluation of their antimicrobial susceptibility

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