Plasmid profile analysis, phage typing, and antibiotic sensitivity of Salmonella dublin from clinical isolates in the United States

Ferris, Kathleen E.; Andrews, Robert E.; Thoen, Charles O.; Blackburn, Billie O.

doi:10.1016/0378-1135(92)90006-f

Cited by 15 publications

(14 citation statements)

References 21 publications

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“…Dublin is a host-adapted Salmonella that is known for its ability to create a carrier state in cattle. 17,21,22 Salmonella ser. Dublin carriers in herds, but no surveys have been performed in Australia.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial susceptibility of Salmonella isolates recovered from calves with diarrhoea in Australia

Izzo

Mohler

House

2011

Australian Veterinary Journal

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OBJECTIVE To identify the common serotypes and antimicrobial resistance patterns of Salmonella spp. associated with diarrhoea in Australian dairy calves under the age of 6 weeks. DESIGN Cross-sectional study. METHODS Faecal samples were collected from outbreaks of diarrhoea in dairy and dairy beef calves less than 6 weeks old. Samples were screened for Salmonella using standard enrichment culture techniques. The antimicrobial susceptibility to 12 commonly used veterinary and human antimicrobials was assessed using the Kirby-Bauer disk diffusion method and the susceptibility profiles of dairy and dairy beef properties were compared using Fisher's exact test. RESULTS Salmonella ser. Dublin, S. ser. Typhimurium and S. ser. Bovismorbificans were the three most common salmonella serotypes isolated. The majority of properties had one serotype. Most of the Salmonella isolates were not resistant to any of the antimicrobials tested. No resistance was seen to amikacin and nalidixic acid, and only one isolate was resistant to ceftiofur or amoxicillin-clavulanic acid. The most common antimicrobial resistance was to streptomycin, ampicillin or combination sulfonamides. Multi-drug resistance was detected in S. ser. Anatum, S. ser. Bovismorbificans, S. ser. Muenster, S. ser. Newport and S. ser. Typhimurium. Isolates from dairy beef properties were more likely to be resistant to ampicillin, kanamycin, neomycin, sulfamethoxazole/trimethoprim and tetracycline (P < 0.05) and were more likely to exhibit multi-drug resistance. CONCLUSION The prevalence of antimicrobial resistance in Salmonella isolates from dairy calves in Australia is low compared with that reported overseas. From a human health perspective, resistance to antimicrobials used in the treatment of human salmonellosis was infrequent.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial susceptibility of Salmonella isolates recovered from calves with diarrhoea in Australia

Izzo

Mohler

House

2011

Australian Veterinary Journal

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“…Application of plasmid profile analysis is a useful typing method for determining relatedness of salmonellae such as S. dublin [19], S. typhimurium [20], S. enteritidis [14] and S. typhi [21], but no such report is available for S. enterica serotype paratyphi A strains. Although we have demonstrated the presence of plasmids in the strains studied, this was not sufficient to establish the relatedness of the isolates.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Susceptibility, Phage Typing and Plasmid Profile of <i>Salmonella enterica</i> Serotype <i>paratyphi A</i> Strains Isolated in Kuwait

et al. 2003

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Objective: To determine the antimicrobial susceptibility, phage type and plasmid profile pattern of Salmonella enterica serotype paratyphi A strains isolated in Kuwait. Material and Methods: From January 1995 to December 1999, 106 strains of S. enterica serotype paratyphi A isolated from an equal number of cases of enteric fever, attending the Infectious Disease and Mubarak Al-Kabeer Hospitals in Kuwait were investigated. The isolates were tested for antimicrobial susceptibility to 8 commonly used antimicrobial agents. Their phage type and plasmid profile patterns were determined using an international set of phages and Qiagen plasmid mini kit, respectively. Results: All of the isolates were susceptible to ciprofloxacin, cefuroxime, ceftazidime, piperacillin and co-trimoxazole. One hundred isolates were susceptible to ampicillin, 99 to chloramphenicol and 98 to tetracycline. None of the isolates was multidrug resistant. Sixty-six percent of the isolates were phage type I, 27.4% phage type II and 6.6% were untypable. All phage type I and untypable strains had 3 plasmids of 2.2, 5 and 20 kb, whereas phage type II strains had only 1 plasmid of 20 kb. Conclusion: The findings indicate that while all of the isolates of the S. enterica serotype paratyphi A were susceptible to 4 of the drugs tested, some were resistant to ampicillin, chloramphenicol or tetracycline, thereby indicating the need for continued surveillance and monitoring of antimicrobial susceptibility of these isolates.

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“…Dublin, there is no widely used phage type scheme, IS200restriction fragment length polymorphism profiles have been reported to be identical in most isolates, plasmid profiling is unsuitable as most isolates contain a single 52 MDa plasmid and pulsed-field gel electrophoresis (PFGE), which is widely used for Salmonella epidemiology, provides limited diversity within Salm. Dublin (Woodward et al 1989;Ferris et al 1992;Olsen and Skov 1994;Liebisch and Schwarz 1996). Therefore, there is a need for a molecular method that can provide a higher degree of polymorphism, so we can study the transmission routes of Salm.…”

Section: Introductionmentioning

confidence: 99%

Multiple-locus variable-number tandem repeat analysis ofSalmonella entericasubsp.entericaserovar Dublin

et al. 2014

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Aims: Salmonella serovar Dublin causes disease in cattle and leads to considerable production losses. In humans, severe invasive disease and high mortality rates are reported. The presently available typing methods provide insufficient discrimination within Salm. Dublin for epidemiological investigations. In this study, we developed a multiple-locus variable-number tandem repeat analysis (MLVA) scheme for high discriminatory typing of Salm. Dublin. Methods and Results: Nine loci of variable number of tandem repeats (VNTRs) were evaluated based on a panel of 40 diverse isolates. The four most discriminative VNTRs were selected for further MLVA analysis. The discriminatory power was evaluated on 272 veterinary and human isolates plus 29 outbreak-related isolates. MLVA divided the 272 isolates into 103 types and successfully identified isolates from an epidemiologically confirmed outbreak. VNTRs exhibited 100% in vitro stability and contained only true repeats. The discriminatory power of the MLVA was compared to pulsed-field gel electrophoresis (PFGE). When analysing a subset of 106 isolates, MLVA obtained 60 types (index of diversity (DI) of 0Á97), while PFGE revealed 10 types (DI of 0Á57). Conclusions:The technique showed a significantly enhanced discriminatory power compared with the current 'gold standard' PFGE. MLVA is a fast and low-cost method. Significance and Impact of the Study: This MLVA method can be recommended to be used in routine subtyping of isolates for outbreak investigations and disease surveillance. The method may provide valuable additional information that can improve the effectiveness of epidemiological investigations of Salm. Dublin infections in patients as well as in the primary production and thereby contribute to the efforts of reducing transmission of infection.

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Plasmid profile analysis, phage typing, and antibiotic sensitivity of Salmonella dublin from clinical isolates in the United States

Cited by 15 publications

References 21 publications

Antimicrobial susceptibility of Salmonella isolates recovered from calves with diarrhoea in Australia

Antimicrobial susceptibility of Salmonella isolates recovered from calves with diarrhoea in Australia

Antimicrobial Susceptibility, Phage Typing and Plasmid Profile of <i>Salmonella enterica</i> Serotype <i>paratyphi A</i> Strains Isolated in Kuwait

Multiple-locus variable-number tandem repeat analysis ofSalmonella entericasubsp.entericaserovar Dublin

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