Multiple-locus variable-number tandem repeat analysis of<i>Salmonella enterica</i>subsp.<i>enterica</i>serovar Dublin

Torpdahl, Mia; Campos, Josefina; Pedersen, Karl; Nielsen, Eva Møller

doi:10.1111/jam.12441

Cited by 12 publications

(20 citation statements)

References 35 publications

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“…Currently published studies have analysed strains of several Salmonella serovars including S . Dublin, which makes it difficult to understand the relationship among strains of this serovar isolated from different sources as well as their genotypic diversity (Kjeldsen, Torpdahl, Campos, Pedersen, & Nielsen, ; Liebana et al., ; Litrup et al., ; Olsen & Skov, ; Zou et al., ).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity, anti‐microbial resistance, plasmid profile and frequency of the Vi antigen in Salmonella Dublin strains isolated in Brazil

Vilela¹,

Frazão

Rodrigues

et al. 2017

Zoonoses and Public Health

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SummarySalmonella Dublin is strongly adapted to cattle causing enteritis and/or systemic disease with high rates of mortality. However, it can be sporadically isolated from humans, usually causing serious disease, especially in patients with underlying chronic diseases.The aim of this study was to molecularly type S. Dublin strains isolated from humans and animals in Brazil to verify the diversity of these strains as well as to ascertain possible differences between strains isolated from humans and animals. Moreover, the presence of the capsular antigen Vi and the plasmid profile was characterized in addition to the anti-microbial resistance against 15 drugs. For this reason, 113 S. Dublin strains isolated between 1983 and 2016 from humans (83) and animals (30) in Brazil were typed by PFGE and MLVA. The presence of the capsular antigen Vi was verified by PCR, and the phenotypic expression of the capsular antigen was determined serologically. Also, a plasmid analysis for each strain was carried out. The strains studied were divided into 35 different PFGE types and 89 MLVA-types with a similarity of ≥80% and ≥17.5%, respectively. The plasmid sizes found ranged from 2 to >150 kb and none of the strains studied presented the capsular antigen Vi. Resistance or intermediate resistance was found in 23 strains (20.3%) that were resistant to ampicillin, ciprofloxacin, chloramphenicol, imipenem, nalidixic acid, piperacillin, streptomycin and/or tetracycline. The majority of the S. Dublin strains studied and isolated over a 33-year period may descend from a common subtype that has been contaminating humans and animals in Brazil and able to cause invasive disease even in the absence of the capsular antigen. The higher diversity of resistance phenotypes in human isolates, as compared with animal strains, may be a reflection of the different anti-microbial treatments used to control S. Dublin infections in humans in Brazil. K E Y W O R D Santigen Vi, anti-microbial resistance, multiple-locus variable-number tandem-repeat analysis, plasmid analysis, pulsed-field gel electrophoresis, Salmonella Dublin

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Section: Introductionmentioning

confidence: 99%

Genetic diversity, anti‐microbial resistance, plasmid profile and frequency of the Vi antigen in Salmonella Dublin strains isolated in Brazil

Vilela¹,

Frazão

Rodrigues

et al. 2017

Zoonoses and Public Health

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“…Since repeat lengths in STTR3 can differ, this locus is sometimes expressed using allele codes as presented in reference 8 or as the total size in base pairs. Similar schemes are also in use or development for other S. enterica serovars, including Enteritidis, Gallinarum, Heidelberg, Dublin, and Typhi (12)(13)(14)(15)(16)(17)(18), and almost a hundred papers reported the use of MLVA for Salmonella analysis between 2004 and 2013 (see the supplemental material).…”

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confidence: 99%

Analysis of Salmonella enterica Serovar Typhimurium Variable-Number Tandem-Repeat Data for Public Health Investigation Based on Measured Mutation Rates and Whole-Genome Sequence Comparisons

et al. 2014

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c Variable-number tandem repeats (VNTRs) mutate rapidly and can be useful markers for genotyping. While multilocus VNTR analysis (MLVA) is increasingly used in the detection and investigation of food-borne outbreaks caused by Salmonella enterica serovar Typhimurium (S. Typhimurium) and other bacterial pathogens, MLVA data analysis usually relies on simple clustering approaches that may lead to incorrect interpretations. Here, we estimated the rates of copy number change at each of the five loci commonly used for S. Typhimurium MLVA, during in vitro and in vivo passage. We found that loci STTR5, STTR6, and STTR10 changed during passage but STTR3 and STTR9 did not. Relative rates of change were consistent across in vitro and in vivo growth and could be accurately estimated from diversity measures of natural variation observed during large outbreaks. Using a set of 203 isolates from a series of linked outbreaks and whole-genome sequencing of 12 representative isolates, we assessed the accuracy and utility of several alternative methods for analyzing and interpreting S. Typhimurium MLVA data. We show that eBURST analysis was accurate and informative. For construction of MLVA-based trees, a novel distance metric, based on the geometric model of VNTR evolution coupled with locus-specific weights, performed better than the commonly used simple or categorical distance metrics. The data suggest that, for the purpose of identifying potential transmission clusters for further investigation, isolates whose profiles differ at one of the rapidly changing STTR5, STTR6, and STTR10 loci should be collapsed into the same cluster.

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“…; Kjeldsen et al . ). Based on these findings, we conclude that MLVA is superior to PFGE in resolving within both Salmonella Dublin and Salmonella Enteritidis.…”

Section: Discussionmentioning

confidence: 97%

“…(), MLVA analysis of 272 Salmonella Dublin strains showed that 85% of the isolates had ≥10 repeat units and that the remaining isolates harboured no repeats (Kjeldsen et al . ). These findings indicate that the risk of ambiguous results due to overlap is low.…”

Section: Discussionmentioning

confidence: 99%

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Development and comparison of a generic multiple-locus variable-number tandem repeat analysis with pulsed-field gel electrophoresis for typing ofSalmonella entericasubsp. enterica

2015

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Multiple-locus variable-number tandem repeat analysis ofSalmonella entericasubsp.entericaserovar Dublin

Cited by 12 publications

References 35 publications

Genetic diversity, anti‐microbial resistance, plasmid profile and frequency of the Vi antigen in Salmonella Dublin strains isolated in Brazil

Genetic diversity, anti‐microbial resistance, plasmid profile and frequency of the Vi antigen in Salmonella Dublin strains isolated in Brazil

Analysis of Salmonella enterica Serovar Typhimurium Variable-Number Tandem-Repeat Data for Public Health Investigation Based on Measured Mutation Rates and Whole-Genome Sequence Comparisons

Development and comparison of a generic multiple-locus variable-number tandem repeat analysis with pulsed-field gel electrophoresis for typing ofSalmonella entericasubsp. enterica

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