Plasmid-Mediated Complement and Phagocytosis Resistance in E. Coli

Fernandez-Beros, Maria Elena; González, Carlos; Kissel, Vincent; Agüero, M E; Binns, M. M.; Fuente, Gabriel de la; Vivaldi, E; Cabello, Felipe C.

doi:10.1007/978-3-642-85718-8_24

Cited by 2 publications

(1 citation statement)

References 17 publications

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“…It may also be possible that genes -and not just isolates -are exchanged between E. coli from different habitats through horizontal transfer; for example, the cvaC gene, which is a marker for conjugative ColV plasmids known to enhance Virulence and resistance genes of B2 E. coli virulence through carriage of virulence genes, was found in several isolates from different origins. Likewise, integronencoding genes, which reside mostly on transposons and conjugative plasmids, were detected among isolates from all isolate origins (Depardieu et al, 2007;Fernandez-Beros et al, 1990;Johnson, 1991).…”

Section: Discussionmentioning

confidence: 95%

Microarray-based detection of extended virulence and antimicrobial resistance gene profiles in phylogroup B2 Escherichia coli of human, meat and animal origin

Jakobsen

Garneau

Kurbasic

et al. 2011

Journal of Medical Microbiology

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Extra-intestinal pathogenic Escherichia coli (ExPEC) causing urinary tract infections (UTIs) most often belong to phylogenetic group B2 and stem from the patient's own faecal flora. It has been hypothesized that the external reservoir for these uropathogenic E. coli in the human intestine may be meat and food-production animals. To investigate such a connection, this study analysed an E. coli phylogroup B2 strain collection (n5161) of geographical and temporally matched isolates, published previously, from UTI patients (n552), community-dwelling humans (n536), imported (n55) and Danish (n513) broiler chicken meat, Danish broiler chickens (n517), imported (n53) and Danish (n527) pork, and healthy Danish pigs (n58). The isolates were subjected to microarray analysis for 315 virulence genes and variants and 82 antimicrobial resistance genes and variants. In total, 133 different virulence and antimicrobial resistance genes were detected in at least one UTI isolate. Between 66 and 87 of these genes were also detected in meat and animal isolates. Cluster analyses of virulence and resistance gene profiles, respectively, showed that UTI and community-dwelling human isolates most often grouped with meat and animal isolates, indicating genotypic similarity among such isolates. Furthermore, B2 isolates were detected from UTI patients and meat, with indistinguishable gene profiles. A considerable proportion of the animal and meat isolates belonged to the ExPEC pathotype. In conclusion, these findings suggest that B2 E. coli from meat and animal origin can be the source of most of the virulence and antimicrobial resistance genes detected in uropathogenic E. coli isolates and that there is a general resemblance of animal, meat and UTI E. coli based on extended gene profiling. These findings support the hypothesis of a zoonotic link between E. coli causing UTIs and E. coli from meat and animals.3Dr Brousseau passed away before the completion of the study.4Present address: Steno Diabetes Center A/S, Niels Steensens

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Section: Discussionmentioning

confidence: 95%

Microarray-based detection of extended virulence and antimicrobial resistance gene profiles in phylogroup B2 Escherichia coli of human, meat and animal origin

Jakobsen

Garneau

Kurbasic

et al. 2011

Journal of Medical Microbiology

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Genotypic and phenotypic determination of five virulence markers in clinical isolates ofEscherichia coli

Bollmann¹,

Seeburg²,

Parschau³

et al. 1997

FEMS Immunol Med Microbiol

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The presence of the virulence markers K1 capsule, serum resistance, aerobactin, S and P/PR fimbriae were examined in a total of 395 E. coli strains from different extraintestinal infections and in 81 faecal isolates of healthy volunteers using specific DNA probes and classical phenotypic methods. All markers were more frequently detected when genotypic assays were applied. The simultaneous occurrence of 3–4 virulence determinants was typical for isolates derived from patients with septicaemia or meningitis. Isolates from blood cultures and cerebrospinal fluid were expressing the virulence phenotypes to a greater extent than isolates from urine or faeces. The use of colony hybridization with specific oligonucleotide and polynucleotide probes for the detection of virulence determinants has been proven to be more specific and reliable than phenotypic approaches.

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Plasmid-Mediated Complement and Phagocytosis Resistance in E. Coli

Cited by 2 publications

References 17 publications

Microarray-based detection of extended virulence and antimicrobial resistance gene profiles in phylogroup B2 Escherichia coli of human, meat and animal origin

Microarray-based detection of extended virulence and antimicrobial resistance gene profiles in phylogroup B2 Escherichia coli of human, meat and animal origin

Genotypic and phenotypic determination of five virulence markers in clinical isolates ofEscherichia coli

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