Genotypic and phenotypic determination of five virulence markers in clinical isolates of<i>Escherichia coli</i>

Bollmann, R.; Seeburg, Anke; Parschau, Jana; Schönian, Gabriele; Sokolowska-Köhler, W; Halle, E.; Presber, W

doi:10.1111/j.1574-695x.1997.tb01020.x

Cited by 4 publications

(3 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Many authors have demonstrated that the majority of neonatal meningitis cases are due to K1-positive E. coli , while other authors have reported that these particular strains are less frequent in vaginal tracts, in a similar proportion between pregnant and non-pregnant women [ 10 , 33 , 34 , 35 ]. Some authors have demonstrated, using DNA hybridization methods, that virulence patterns (including K1-capsules) of strains isolated from blood culture and cerebrospinal fluid were different from those of urogenital strains [ 36 ]. This observation is nowadays contested, due to similar representation, as in the present study, of K1-positive and K1 negative strains (40% and 60% approximately in the present study).…”

Section: Discussionmentioning

confidence: 99%

K1 Antigen Is Associated with Different AST Profile in Escherichia coli: A One-Month-Long Pilot Study

et al. 2021

View full text Add to dashboard Cite

Escherichia coli is responsible for diseases of varying severity. The “K” antigen designates the capsular polysaccharides on the bacterial surface, which are mostly similar to those of highly pathogenic bacteria. The K1 antigen is often found in pathogenic E. coli. Aim: While the published studies on the AST profile of K1-positive E. coli have focused on pregnant women or newborns, this study aimed to characterize the AST profile of K1-positive E. coli independently of the clinical sample of isolation. Over a 4-week-long period, all patients hospitalized/consulting at the Poitiers University Hospital presenting a determined AST on E. coli were prospectively included to define their K1-status (Pastorex Meningitis) and to collect the clinical (age/sex) or biological metadata (AST/MIC). Among the 296 included samples, no differential representation was observed between K1 results regarding sample nature. K1-negative results were associated with multiple antibiotic-resistance (12.3% vs. 33.0%; p < 0.01). AST phenotypes differed between these groups, with a higher proportion of K1-negativity among resistant strains, especially on β-lactams (ureidopenicillin, 25.8% vs. 14.9%; and ampicillin/inhibitor, 50.0% vs. 26.8%; p < 0.05) or quinolone (19.8% vs. 7.0%) and sulfamethoxazole-trimethoprim (30.2% vs. 12.3%) (p < 0.01). This study analyzed E. coli ASTs in clinical samples of all types, regarding their K1-antigen status.

show abstract

Section: Discussionmentioning

confidence: 99%

K1 Antigen Is Associated with Different AST Profile in Escherichia coli: A One-Month-Long Pilot Study

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Важность жгутикового аппарата была подтверждена экспериментально: при нарушении функции генов, кодирующих такие белки, как FliD, FlaA и FlaB, происходит снижение способности H. pylori к колонизации слизистой оболочки желудка [13,14]. Важную роль в этом процессе также играет хемотаксис на различные молекулы-хемоаттрактанты: муцин, бикарбонат натрия, мочевину и различные аминокислоты, которые распознаются хеморецепторами возбудителя T1pA, B, C и D, CheA киназой и другими, существование которых установлено в многочисленных экспериментальных исследованиях [13,15,16,17].…”

Section: результаты б иологические особенности H Pylori и патогенез хеликобактерной инфекцииunclassified

Modern knowledge on pathogenesis, diagnosis and treatment of helicobacter infection

Kiselev

Gorlenko

El‐Taravi

et al. 2021

jour

View full text Add to dashboard Cite

Since its discovery, H. pylori infection is known as one of the risk factor for the development of gastritis, peptic ulcer, GIT tumors and numerous other diseases such as psoriasis. Infection caused by H. pylori is posed as the top oncogene in the risk of the development of gastrocarcinoma (First class oncogene by Classification of International Agency for Research of Cancer). That is why the elaboration of fast and accurate methods of diagnosis (non-invasive methods especially) and proper treatment of Helicobacter infection is still very important. Throughout the time, knowledge about pathogenesis of Helicobacter infection have been expanded with the detection of adhesins, chemotaxins and multiple virulence factors related to invasion, adhesion and cytotoxicity of H. pylori. Invasive and non-invasive methods of diagnostics are currently being improved in effectiveness and accuracy. But still, due to different factors (e. g., dramatically increasing drug resistance), eradication of H. pylori remains big problem world-wide. Our review represents modern data on pathogenesis, diagnostics and treatment of Helicobacter infection.

show abstract

“…The pathogenesis of E. coli pyelonephritis is complex, involving the interaction of several factors present in the host and in the invading organism. Host factors predisposing to PN can be anatomic, increased uroepithelial cell adherence, and nonsecretion of P blood group or of the Lewis blood group antigens[4]. Previous investigations have indicated that various virulence factors, such as pili associated with pyelonephritis (pap), afimbrial adhesin I, haemolysin (hly) and cytotoxic necrotizing factor 1 (cnf 1) are useful markers for the detection of uropathogenic E. coli and could therefore be used in the diagnosis of PN[5–7].…”

Section: Frequency Of O Groups In Children With Urinary Tract Infectimentioning

confidence: 99%

Virulence factors in Escherichia coli from children with pyelonephritis

Draghijeva¹

2000

Clinical Microbiology and Infection

View full text Add to dashboard Cite

The occurrence of bacterial infections of the urinary tract in children is exceeded in frequency only by bacterial infections of the respiratory tract. Acute pyelonephritis represents the most severe type of urinary tract infection in children. It not only results in greater morbidity but also has great potential for causing irreversible damage, including renal scarring, hypertension before the age of 30 years and pregnancy complications [1][2][3]. Escherichia coli causes most pyelonephritis (PN)-approximately 80-90% of first infections in children. The pathogenesis of E. coli pyelonephritis is complex, involving the interaction of several factors present in the host and in the invading organism. Host factors predisposing to PN can be anatomic, increased uroepithelial cell adherence, and nonsecretion of P blood group or of the Lewis blood group antigens [4]. Previous investigations have indicated that various virulence factors, such as pili associated with pyelonephritis (pap), afimbrial adhesin I, haemolysin (hly) and cytotoxic necrotizing factor 1 (cnf 1) are useful markers for the detection of uropathogenic E. coli and could therefore be used in the diagnosis of PN [5][6][7].The aim of this study was to determine the role of virulence factors in E. coli urinary isolates from children with pyelonephritis in University Children's Hospital, Sofia. One hundred and eighty E. coli strains were isolated from the urine of hospitalized children, ages between 5 months and 16 years of age, in the University Children's Hospital, Sofia. Midstream urine samples were collected after washing the external genitalia with sterile water.One hundred children had clinical acute pyelonephritis, with symptoms: fever, temperature ×38.5°C, flank pain, frequent chills, dysuria, frequency of urination, positive urine culture (×10 5 bacteria/mL urine), pyuria (×10 white blood cells/high power field), elevated erythrocyte sedimentation rate (×20 mm/h), an increased concentration of C-reactive protein (×20 mg/L) and reduced concentrating capacity. The clinical diagnosis of cystitis and urethritis was based on a history of frequency and urgency of urination, dysuria, suprapubic pain, and significant bacteriuria with the absence of fever, chills, and flank pain, and normal renal function.The control group comprised of 80 sex-and age-matched healthy children who had sterile urine cultures. Their E. coli strains were isolated from normal intestinal flora.Identification of E. coli was by standard bacteriological

show abstract

Genotypic and phenotypic determination of five virulence markers in clinical isolates ofEscherichia coli

Cited by 4 publications

References 24 publications

K1 Antigen Is Associated with Different AST Profile in Escherichia coli: A One-Month-Long Pilot Study

K1 Antigen Is Associated with Different AST Profile in Escherichia coli: A One-Month-Long Pilot Study

Modern knowledge on pathogenesis, diagnosis and treatment of helicobacter infection

Virulence factors in Escherichia coli from children with pyelonephritis

Contact Info

Product

Resources

About