Plasma surface modification of poly(D,L‐lactic acid) as a tool to enhance protein adsorption and the attachment of different cell types

Abstract

We have studied the influence of oxygen radio frequency glow discharge (RfGD) on the surface and bulk properties of poly(D,L-lactic acid) (PDLLA) and the effect of this surface modification on both protein adsorption and bone cell behavior. PDLLA films were characterized before and after plasma surface modification by water contact angle, surface energy, and adhesion tension of water as well as by scanning electron microscopy (SEM), X-ray electron spectroscopy (XPS), and Fourier transform infra-red (FTIR) spectroscopy. RfGD-films showed an increase in hydrophilicity and surface energy when compared with untreated films. Surface morphological changes were observed by SEM. Chemical analysis indicated significant differences in both atomic percentages and oxygen functional group. Protein adsorption was evaluated by combining solute depletion and spectroscopic techniques. Bovine serum albumin (BSA), fibronectin (FN), vitronectin (VN), and fetal bovine serum (FBS) were used in this study. RfGD-treated surfaces adsorbed more BSA and FN from single specie solutions than FBS that is a more complex, multi-specie solution. MG63 osteoblast-like cells and primary cultures of fetal rat calvarial (FRC) cells were used to assess both the effect of RfGD treatment and protein adsorption on cell attachment and proliferation. In the absence of preadsorbed proteins, cells could not distinguish between treated and untreated surfaces, with the exception of MG63 cells cultured for longer periods of time. In contrast, the adsorption of proteins increased the cells' preference for treated surfaces, thus indicating a crucial role for adsorbed proteins in mediating the response of osteogenic cells to the RfGD-treated PDLLA surface.

Vitamin E acetate addition to poly(d,l)lactic acid modifies its mechanical behavior without affecting biocompatibility

“…Cell proliferation and differentiation onto implanted biomaterials surface are the major hallmark of material biocompatibility, which is the most fundamental and crucial concern for designing implantable biomaterials. 3,[38][39][40][41] As 10% Vit. E and Vit.…”

Effect of oxygen plasma on surface properties and biocompatibility of PLGA films

“…The poly(methyl methacrylate) (PMMA) films were subjected to different oxygen plasma treatments and it was concluded that if there is a relation between SFE and cell attachment, value of SFE should be around 61 mJ/m 2 for maximum cell attachment. [17] In the study of Alves et al [9] the effect of surface modification by oxygen RF glow discharge on protein adsorption and bone cell behavior were discussed. The results showed that the oxygen plasma enhanced surface wettability and hydrophilicity and the proliferation of MG63 osteoblast-like cells on P(D,L-LA) surfaces.…”

Bioactive albumin functionalized polylactic acid membranes for improved biocompatibility

“…Although PLA has been approved for these applications [4,9], several studies have also shown that contact of some of the PLA based products with human body is associated with biocompatibility problems including inducing nodules and foreign-body granulomas [10] and post-implantation inflammation [11]. PLA hydrophobicity has also been shown to affect cell attachment, viability and proliferation [12][13][14][15]. Hence, many strategies including those employing natural materials like gelatin, collagen, alginate or biotin for coating PLA polymers are being intensely investigated to improve the biocompatibility of PLA based biomaterials [16,17].…”