Plasma Surface Modification of Chitosan Membranes: Characterization and Preliminary Cell Response Studies

Abstract

Surface modification of biomaterials is a way to tailor cell responses whilst retaining the bulk properties. In this work, chitosan membranes were prepared by solvent casting and treated with nitrogen or argon plasma at 20 W for 10-40 min. AFM indicated an increase in the surface roughness as a result of the ongoing etching process. XPS and contact angle measurements showed different surface elemental compositions and higher surface free energy. The MTS test and direct contact assays with an L929 fibroblast cell line indicated that the plasma treatment improved the cell adhesion and proliferation. Overall, the results demonstrated that such plasma treatments could significantly improve the biocompatibility of chitosan membranes and thus improve their potential in wound dressings and tissue engineering applications.

Using Plasma Treatment for Enhancing the Coating for Rechargeable Antimicrobial Finishing of Cotton Fabric

“…Therefore, the optimal chlorinated time is 20 min for cotton fabric coated with DMH to transfer the amino groups into N-halamine structures. When compare this fabric with the fabric without plasma treatment, it can be seen that the concentration of active chlorine of the cotton fabric with plasma treatment is higher, that is, plasma treatment enhances the coating effect of DMH and N 2 plasma treatment introduces nitrogen groups into the surface of cotton fabric, because the etching effect of plasma treatment makes the surface of fibers uneven which provides convenience for DMH coating, meanwhile, N 2 plasma can functionalize the substrate with N-containing group, such as -NH 2 , -NH, =NH, CONH 2 or C≡N [5]. Fig.…”

Regenerable Antibacterial Cotton Fabric by Plasma Treatment with Dimethylhydantoin: Antibacterial Activity against S. aureus

“…In this study, it was found that N 2 plasma treatment functionalises the cotton fabric with N-containing groups and C=O groups, according to the FTIR study [43]. The formation of carboxyl groups on fabric after plasma treatment is caused by the oxidation of hydroxyl groups by particles in N 2 plasma, and they can react with amide groups in a hydantoin ring [44].…”

Novel Chitin and Chitosan Materials in Wound Dressing

“…The MTS test and direct contact assays with an L929 fibroblast cell line indicated that the plasma treatment improved the cell adhesion and proliferation. Overall, the results demonstrated that such plasma treatments could significantly improve the biocompatibility of chitosan membranes and thus improve their potential in wound dressings and tissue engineering applications (Silva et al, 2008). HemCon® bandage is an engineered chitosan acetate preparation designed as a hemostatic dressing, and is under investigation as a topical antimicrobial dressing (Burkatovskaya et al, 2008).…”

“…Surface modification of biomaterials is another way to tailor cell responses whilst retaining the bulk properties. Silva et al (2008) prepared chitosan membranes by solvent casting and treated with nitrogen or argon plasma at 20W for 10-40 min. Atomic Force Microscopy analysis (AFM) indicated an increase in the surface roughness as a result of the etching process.…”