Plasma Renalase is Not Associated with Blood Pressure and Brachial-Ankle Pulse Wave Velocity in Chinese Adults With Normal Renal Function

Abstract

Background/Aims: This study aimed to investigate the association of renalase with blood pressure (BP) and brachial-ankle pulse wave velocity (baPWV) in order to better understand the role of renalase in the pathogenesis of hypertension and atherosclerosis. Methods: A total of 344 subjects with normal kidney function were recruited from our previously established cohort in Shaanxi Province, China. They were divided into the normotensive (NT) and hypertensive (HT) groups or high baPWV and normal baPWV on the basis of BP levels or baPWV measured with an automatic waveform analyzer. Plasma renalase was determined through an enzyme-linked immunosorbent assay. Results: Plasma renalase did not significantly differ between HT and NT groups (3.71 ± 0.69 µg/mL vs. 3.72 ± 0.73 μg/mL, P = 0.905) and between subjects with and without high baPWV (3.67 ± 0.66 µg/mL vs. 3.73 ± 0.74 µg/mL, P = 0.505). However, baPWV was significantly higher in the HT group than in the NT group (1460.4 ± 236.7 vs. 1240.7 ± 174.5 cm/s, P < 0.001). Plasma renalase was not correlated with BP levels and baPWV in the entire group. Linear and logistic regression analysis revealed that plasma renalase was not significantly associated with hypertension and high baPWV. Conclusion: Plasma renalase may not be associated with BP and baPWV in Chinese subjects with normal renal function.

Relationship between brachial-ankle and heart-femoral pulse wave velocities and the rapid decline of kidney function

“…Wang et al . reported that the eGFR was associated with decreased odds of a high baPWV (OR 0.974, P = 0.04) in 344 Chinese adults 34 . In their study, people with high baPWV showed higher urine albumin-to-creatinine ratio (7.52 mg/g) than those with normal baPWV (vs. 6.07 mg/g, P = 0.021), but the significance was not maintained in multivariate logistic regression analysis.…”

Association of Uric Acid in Serum and Urine with Arterial Stiffness: Hanzhong Adolescent Hypertension Study

“…Alcohol consumption was defined as subjects who claimed that they consumed alcohol (liquor, beer, or wine) every day and that consumption lasted for 6 months [27]. Blood pressure (BP) was measured in the sitting position using a standard mercury sphygmomanometer as previously described [28][29][30][31]. Hypertension was defined as a systolic BP of ≥140 mm Hg, a diastolic BP ≥ 90 mm Hg, or as the use of antihypertensive agents according to subjects' self-report or pharmacy data.…”

A new method for quantitative determination of renalase based on mass spectrometric determination of a proteotypic peptide labelled with stable isotopes

“…Such contradictory results can be attributed to the fact that all currently existing methods for quantitative determination renalase are based on employment of various antibodies . Reasons for these differences in the antibody‐based evaluations of renalase levels have been considered by Desir and Peixoto and provoked serious concerns about ‘proper interpretation’ of plasma renalase levels ‘especially in patients with kidney disease’ . These include insufficient specificity of generated antibodies to renalase and their interaction not only with full‐length renalase, but also with renalase‐derived products of both degradation and aggregation …”

“…5,6 Reasons for these differences in the antibody-based evaluations of renalase levels have been considered by Desir and Peixoto 5 and provoked serious concerns about 'proper interpretation' of plasma renalase levels 'especially in patients with kidney disease' . 13 These include insufficient specificity of generated antibodies to renalase and their interaction not only with full-length renalase, but also with renalase-derived products of both degradation and aggregation. 5,13 Thus there is a clear need for antibody-free methods of renalase detection.…”

“…13 These include insufficient specificity of generated antibodies to renalase and their interaction not only with full-length renalase, but also with renalase-derived products of both degradation and aggregation. 5,13 Thus there is a clear need for antibody-free methods of renalase detection. Recently we successfully used mass spectrometry for qualitative detection of renalase in human urine 14,15 and cultivated HEK293T cells.…”