Plasma proteins as early biomarkers of exposure to carcinogenic aromatic amines

Miller, Mark J.; Parmelee, David C.; Benjamin, Timothy; Sechi, Salvatore; Dooley, Kenneth L.; Kadlubar, Fred F.

doi:10.1016/0009-2797(94)90021-3

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…1, no. 11, 36/38 kDa pI 5.8+6.0 similar to plasma haptoglobin; Miller et al 1994). Recently, we described that maternal haptoglobin is incorporated into the extraembryonic matrices and is enriched within the blastocyst fluid (Herrler et al 2001).…”

Section: Discussionmentioning

confidence: 95%

Proteins in the extraembryonic matrix of preimplantation rabbit embryos

Herrler

Wolff²,

Beier

2002

Anatomy and Embryology

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Preimplantation embryos of several species are surrounded by an extraembryonic matrix (often simply named zona pellucida) until briefly before implantation. All signals of the early embryo-maternal dialogue have to pass this matrix and therefore are detectable inside. We investigated the protein pattern of the extraembryonic matrices of 3-6-day-old rabbit embryos by two-dimensional gel-electrophoresis. Using (35)S-methionine incorporation, embryonic proteins were labelled and could be distinguished from maternal proteins. Furthermore, the presence of three different proteins (insulin-like growth factor-binding protein-3, uteroglobin, haptoglobin) within the matrices of day-6 embryos was investigated by Western blot analysis. The pattern and numbers of protein spots detected was clearly dependent on the time of embryonic development. Of all proteins detected, 19.3% and 33% are of embryonic origin (day 5 and day 6, respectively). At day 4 the zona proteins are no longer detectable, reflecting the degradation of the zona pellucida. From day 4 to day 5 proteins detectable within the extraembryonic matrices increase enormously. This demonstrates that embryo-maternal signalling accelerates at least 2 days before implantation. Insulin-like growth factor-binding protein-3, uteroglobin and haptoglobin are part of the early signalling as shown by Western blot analysis. Insulin-like growth factor-binding protein-3 could be detected as one spot at 38 kDa pI 6.1, uteroglobin at 8 kDa pI 6.0 and haptoglobin as two spots/isoforms at 36/38 kDa pI 5.8 and pI 6.0. These results demonstrate that extraembryonic matrices serving as a mailbox are a valuable tool for investigating early embryo-maternal signalling.

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Section: Discussionmentioning

confidence: 95%

Proteins in the extraembryonic matrix of preimplantation rabbit embryos

Herrler

Wolff²,

Beier

2002

Anatomy and Embryology

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“…This study investigated plasma proteins as early biomarkers of exposure to carcinogenic aromatic amines inducing bladder cancer [60]. Since serum is not the ideal sample for 2D-GE, as previously discussed, most studies based on 2D-GE and bladder cancer were carried out with cell culture or tissue samples.…”

Section: Gel-based Approachesmentioning

confidence: 99%

“…At the time of presentation, 60.7% of transitional cell bladder cancers show a low grade, nonmuscle invasive phenotype (grade 1 or 2 [low-grade (LG)], pTa or pT1), representing a manageable and not immediately life-threatening stage of disease [2]. The 15-year progression-free survival rate in patients with LG noninvasive papillary tumors (pTa) is 95% without any cancer-specific mortality [3].…”

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confidence: 99%

Tissue and serum proteomic profiling for diagnostic and prognostic bladder cancer biomarkers

Schwamborn

Gaisa

Henkel

2010

Expert Review of Proteomics

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A panel of biomarkers for the early detection of bladder cancer has not yet been identified. Many different molecules, including DNA, RNA or proteins have been reported but none have provided adequate sensitivity for a single-tier screening test or a test to replace cystoscopy. Therefore, multimarker panels are discussed at present to give a more-precise answer to the biomarker quest. Mass spectrometry or 2D gel-electrophoresis have evolved greatly within recent years and are capable of analyzing multiple proteins or peptides in parallel with high sensitivity and specificity. However, transmission of screening results from one laboratory to another is still the main pitfall of those methods; a fact that emphasizes the need for consistent and standardized procedures as suggested by the Human Proteome Organization (HUPO). In this article, recent results in screening approaches and other proteomic techniques used for biomarker evaluation in bladder cancer are discussed with a focus on serum and tissue biomarkers.

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Proteins of rat serum: I. Establishing a reference two‐dimensional electrophoresis map by immunodetection and microbore high performance liquid chromatography‐electrospray mass spectrometry

et al. 1998

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In the present investigation, we have identified 56 major spots, or spot rows, corresponding to 22 proteins, in the 2-DE pattern of adult male rats. This was done mainly by applying two complementary techniques, namely immunoblotting and high performance liquid chromatography-mass spectrometry (HPLC-MS) peptide mapping. Glycoproteins were characterized by affinity blotting with six lectins. We have also detailed how rat serum differs from human serum in two main respects: (i) relative abundance of individual proteins, which amounts in some cases to a complete absence in either sample, and (ii) varying molecular parameters for homologous proteins. It was thus possible to establish a first-generation reference map of rat serum proteins, which can be accessed through http://weber.u.washington.edu/ruedilab/aebersold++ +.html. We hope the present database will be a useful reference for the evaluation of changes in serum protein distribution in the course of pharmacological and toxicological studies. The recognition of species-specific proteins appears of special relevance in this respect.

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Plasma proteins as early biomarkers of exposure to carcinogenic aromatic amines

Cited by 4 publications

References 13 publications

Proteins in the extraembryonic matrix of preimplantation rabbit embryos

Proteins in the extraembryonic matrix of preimplantation rabbit embryos

Tissue and serum proteomic profiling for diagnostic and prognostic bladder cancer biomarkers

Proteins of rat serum: I. Establishing a reference two‐dimensional electrophoresis map by immunodetection and microbore high performance liquid chromatography‐electrospray mass spectrometry

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