Plasma Proteins As Biodosimetric Markers of Low-Dose Radiation in Mice

Ge, Chang‐Hui; Liang, Yanjie; Zhang, Yaocang; Su, Fei; Chen, Lu; Ma, Feiyue; Ding, Kexin; Zhu, Jie; Zheng, Xiaofei; Fu, Hanjiang

doi:10.1177/15593258211016257

Cited by 2 publications

(3 citation statements)

References 35 publications

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“…While our study did not uncover the other proteins identified by Fu et al, it is likely that low abundant interleukins would be less likely to be detected by the 2-D gel method. Another recent study observing long term exposure to low-dose radiation found interleukin IL-5, IL-12p40, P-selectin, and serum amyloid A1 to be good predictors of low-dose radiation exposure with biodosimetric potential 46 .…”

Section: Discussionmentioning

confidence: 99%

Gamma-tocotrienol, a radiation countermeasure, reverses proteomic changes in serum following total-body gamma irradiation in mice

Rosen

Fatanmi

Wise

et al. 2022

Sci Rep

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Radiological incidents or terrorist attacks would likely expose civilians and military personnel to high doses of ionizing radiation, leading to the development of acute radiation syndrome. We examined the effectiveness of prophylactic administration of a developmental radiation countermeasure, γ-tocotrienol (GT3), in a total-body irradiation (TBI) mouse model. CD2F1 mice received GT3 24 h prior to 11 Gy cobalt-60 gamma-irradiation. This dose of radiation induces severe hematopoietic acute radiation syndrome and moderate gastrointestinal injury. GT3 provided 100% protection, while the vehicle control group had 100% mortality. Two-dimensional differential in-gel electrophoresis was followed by mass spectrometry and Ingenuity Pathway Analysis (IPA). Analysis revealed a change in expression of 18 proteins in response to TBI, and these changes were reversed with prophylactic treatment of GT3. IPA revealed a network of associated proteins involved in cellular movement, immune cell trafficking, and inflammatory response. Of particular interest, significant expression changes in beta-2-glycoprotein 1, alpha-1-acid glycoprotein 1, alpha-2-macroglobulin, complement C3, mannose-binding protein C, and major urinary protein 6 were noted after TBI and reversed with GT3 treatment. This study reports the untargeted approach, the network, and specific serum proteins which could be translated as biomarkers of both radiation injury and protection by countermeasures.

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Section: Discussionmentioning

confidence: 99%

Gamma-tocotrienol, a radiation countermeasure, reverses proteomic changes in serum following total-body gamma irradiation in mice

Rosen

Fatanmi

Wise

et al. 2022

Sci Rep

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show abstract

“…Radiationinduced plasma protein expression has been reported as a possible radiometric dosimeter. 40 In the following experiments, we performed linear regression, second order polynomial, and third order polynomial analysis on the expression of five candidate genes after radiation. The expression of PC4 had the best correlation with radiation dose.…”

Section: Discussionmentioning

confidence: 99%

“…Radiation-induced plasma protein expression has been reported as a possible radiometric dosimeter. 40…”

Section: Discussionmentioning

confidence: 99%

Positive Cofactor 4 as a Potential Radiation Biodosimeter for Early Assessment

Gong

Liu

et al. 2022

Dose-Response

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During a major radiation event, a large number of people need to be rapidly assessed for radiation damage to ensure effective medical treatment and efficient use of medical resources. However, current techniques cannot meet the requirement of rapid detection of large quantities of samples in an emergency. It is essential to develop rapid and accurate radiation biodosimeters in peripheral blood. Here, we identified radiation sensitive genes in mice by RNA sequencing and evaluated their utility as radiation biodosimeters in human cell lines. Mice were subjected to gamma-irradiation with different doses (0–8 Gy, .85 Gy/min), and the tail venous blood was analyzed by RNA sequencing. We have identified 5 genes with significantly differential expression after radiation exposure. We found that positive cofactor 4(PC4) had well correlation with radiation dose in human lymphoblastoid cell line after irradiation. The relative expression of PC4 gene showed a good linear correlation with the radiation dose after 1–5 Gy irradiation (.85 Gy/min). PC4 gene can be rapidly recruited to the DNA damage sites faster than γ-H2AX after radiation in immunofluorescence detection. In conclusion, PC4 may be represented as new radiation biological dosimeter for early assessment.

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Plasma Proteins As Biodosimetric Markers of Low-Dose Radiation in Mice

Cited by 2 publications

References 35 publications

Gamma-tocotrienol, a radiation countermeasure, reverses proteomic changes in serum following total-body gamma irradiation in mice

Gamma-tocotrienol, a radiation countermeasure, reverses proteomic changes in serum following total-body gamma irradiation in mice

Positive Cofactor 4 as a Potential Radiation Biodosimeter for Early Assessment

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