Plasma phospholipid profiling of a mouse model of anxiety disorder by hydrophilic interaction liquid chromatography coupled to high‐resolution mass spectrometry

Abstract

Glycerophospholipids (PLs), as amphipathic small molecules and the main constituents of biological membranes, play an important role in several cellular processes, even though their accurate identification from complex biological samples remains a challenge. In this paper, we report a fast and comprehensive HILIC-ESI-MS method for the analysis of glycerophospholipid classes using high-resolution mass spectrometry in negative mode. The final method enabled the quantitative analysis of 130 endogenous PL species in mouse plasma. The application of the method developed was to find differences of plasma PL composition in a mouse model of anxiety disorder. In the case of four PL classes and 35 PL species, significant differences were observed comparing low anxiety-related behavior with high anxiety-related behavior groups. The most characteristic trend was up-regulation in both the PL classes and PL species, and decreases were only detected in two phosphatidylcholines among 35 species in mice having elevated anxiety.

Anxiety Disturbs Metabolome of Blood Plasma in Coronary Heart Disease Patients

“…However, when atherosclerotic diseases damage endothelial cells, blood vessels are unable expand properly, and exposure to 5hydroxytryptamine will resulted in vasoconstriction. This may be the underlying mechanism of the association between increased serotonin blood levels and cardiac events in coronary heart disease 11 . In this study thromboxane B2 metabolism was increased in anxiety group.…”

“…These substance including lysoPC (16:0), lysoPC (15:0), lysoPC glycerophospholipid metabolism disorders decreased in the anxiety group 25,26 . Robert et al analyzed the plasma phospholipid spectrum of 31 mice models with LC and hydrophilic interaction liquid chromatography and high-resolution mass spectrometry, and found that the most obvious upregulation was phosphatidylinositol (PE) and lysophosphatidylethanolamine (LPE) 11 . Although these phospholipids are different from the phospholipid compounds in this study, they all provide theoretical support for the hypothesis that membrane lipids play a key role in anxiety related diseases at the animal.…”

“…Hyperlipidemia is closely related to stress and anxiety 28 . Sympathetic activation in generalized anxiety disorder increases lipoprotein lipase activity through the release of adrenaline and corticosteroids 11 . This hyperactivity in lipoprotein lipase results in an increase in free fatty acids, which can be converted into cholesterol and triglycerides.…”

“…Recent research found that changes in several metabolites, including certain amino acids, products of pyrimidine metabolism and the pentose phosphate pathway, were observed in ACS patients 2,[8][9][10] . There are also metabolic disorders in anxiety 11,12 which aggravate coronary artery disease.…”

Anxiety disturbs the blood plasma metabolome in acute coronary syndrome patients

“…Recent research revealed that changes in several metabolites, including certain amino acids, products of pyrimidine metabolism and the pentose phosphate pathway, were observed in ACS patients 2 , 11 – 13 . Metabolic disorders were also associated with anxiety 14 , 15 , which can aggravate coronary artery disease. Metabolites related to oxidative stress, inflammatory processes, lipid and energy metabolism, glutamine metabolism, and neurotransmission may have the potential to serve as biomarkers for anxiety disorders 16 .…”