Plasma P selectin (GMP-140) and glycocalicin are elevated in preeclampsia and eclampsia: Their significances

Halim, Abdul; Kanayama, Naohiro; Maradny, Emad El; Nakashima, Akira; Bhuiyanb, A.B.; Khatun, Selina; Terao, Toshihiko

doi:10.1016/s0002-9378(96)70407-6

Cited by 70 publications

(37 citation statements)

References 21 publications

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“…This slower increase in GAC in our study can probably be explained by the very low plasma concentrations, resulting in low amounts of plasma proteases (i.e. calpain, plasmin, trypsin and elastase) [22, 23]. These proteases cleave GpIbα, generating soluble GC in the supernatant.…”

Section: Discussionmentioning

confidence: 74%

Increase in Glycocalicin Levels in Platelet Concentrates Stored in Plasma or Synthetic Medium for 8 Days: Comparison with Other Platelet Activation Markers

et al. 2000

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Background and Objectives: Glycocalicin (GC) is a proteolytic fragment of GpIb and can conveniently be measured in supernatants of platelet concentrates (PCs) by means of a sandwich ELISA. Because of the convenience of the assay and easy sample storage, we tested its suitability as a sensitive platelet activation parameter during PC storage. Material and Methods: Filtered PCs in plasma or additive solution were made from 5 pooled buffy coats and were subsequently stored during 8 days at 22±2°C. Correlation coefficients (r) were calculated after comparison of GC levels with platelet parameters. Results: A significant increase in GC concentration was found on all subsequent sampling days. PC stored in plasma showed GC levels that correlated well with the soluble P–selectin levels (r = 0.7506), P–selectin (CD62P) expression on platelet membranes (r = 0.8843), morphology scores according to Kunicki (r = –0.7102), lactate concentrations (r = 0.9216), glucose concentrations (r = –0.8913) and β–thromboglobulin (β–TG) concentrations (r = 0.8913). In PCs stored in additive solution, the correlation coefficients with these markers were 0.9209 with soluble P–selectin, 0.7161 with CD62P expression, –0.7474 with morphology score, –0.8908 with glucose concentrations, 0.8923 with lactate concentrations and 0.8908 with β–TG concentrations. Conclusions: The GC concentration correlates well with sensitive platelet (activation) parameters, rendering it a sensitive and convenient parameter for platelet activation.

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Section: Discussionmentioning

confidence: 74%

Increase in Glycocalicin Levels in Platelet Concentrates Stored in Plasma or Synthetic Medium for 8 Days: Comparison with Other Platelet Activation Markers

et al. 2000

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“…P-selectin-deficient mice exhibit severely diminished early (two to four hours) leukocyte rolling and delayed recruitment of leukocytes in various inflammatory settings (10)(11)(12). In contrast, soluble P-selectin, which is present in the plasma of normal subjects (13)(14)(15) and elevated in patients with GN (16) and other diseases (13,15,(17)(18)(19)(20), inhibits the adhesion of activated neutrophils to endothelial cells (21), as well as the neutrophil respiratory burst (22).…”

Section: Introductionmentioning

confidence: 99%

P-selectin deficiency exacerbates experimental glomerulonephritis: a protective role for endothelial P-selectin in inflammation

Rosenkranz¹,

Mendrick²,

Cotran³

et al. 1999

J. Clin. Invest.

117

123

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P-selectin is a leukocyte adhesion receptor present in endothelial cells and platelets. We examined the role of P-selectin in the autologous phase of an accelerated model of anti-glomerular basement membrane (GBM) glomerulonephritis using P-selectin-deficient mice and chimeric mice expressing P-selectin only in platelets or endothelial cells. P-selectin-deficient mice exhibited more severe glomerular damage with increased interstitial mononuclear leukocytic infiltrates, and had significantly increased proteinuria and mortality when compared to wild-type mice. P-selectin on the endothelium was predominantly responsible for protection from the exacerbated disease, because chimeric mice with endothelial Pselectin, and not mice with platelet P-selectin, showed glomerular injury similar to that in wild-type animals. Levels of soluble circulating P-selectin were increased in nephritic wild-type mice and in chimeric mice with endothelial P-selectin, but not platelet P-selectin. Levels of soluble P-selectin, which has been shown to be anti-inflammatory in vitro, were inversely associated with the severity of disease. P-selectin was not expressed in the endothelium of the glomerulus or interstitium. Thus, the protective effect in wild-type mice may be accounted for, in part by soluble P-selectin shed by non-renal endothelial cells, although other endothelial P-selectin-dependent mechanisms cannot be ruled out.

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“…Some studies reported an increase of sPselectin, sE-selectin, and sICAM-1 [18][19][20]. While others reported no changes [21,22].…”

Section: Discussionmentioning

confidence: 99%

A comparative study of vascular cell adhesion molecule-1 and high-sensitive C-reactive protein in normal and preeclamptic pregnancies

Farzadnia

Ayatollahi

Hasan-Zade

et al. 2013

IMAS

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Abstract:Background: Preeclampsia is characterized by hypertension, dyslipidemia, and systemic inflammatory response. The aim of this study was to determine the level of serum level of soluble vascular cell adhesion molecule-1 (sVCAM-1) and high-sensitive C-reactive protein (hsCRP) in preeclampsia and to compare normal pregnant, mild preeclamptic, and severe preeclamptic women. Methods: A cross-sectional study was conducted to determine the plasma concentrations of sVCAM-1 and hsCRP in peripheral blood obtained from normal pregnant (n=40), mild preeclamptic (n=37), and severe preeclamptic women (n=38). A concentration of soluble adhesion molecule was determined with enzymelinked immunosorbent assay. hsCRP was measured with immunoturbidometric. Results: There was significant difference in the means serum hsCRP between normal pregnant women and mild preeclamptic women (P<0.05). Serum concentration of hsCRP and sVCAM-1 (ng/mL) were significantly higher in severe preeclampsia (P<0.05) than normal pregnancy. There were also significant differences in hsCRP and sVCAM-1 levels between mild and severe (P<0.05). There was no difference in the mean sVCAM-1 between normal pregnant and mild preeclamptic women. Conclusion: We have determined the serum concentration of VCAM-1 and hsCRP in normal pregnancy and preeclampsia. sVCAM-1 is elevated in severe preeclampsia compared with normal pregnancy, and hsCRP is elevated in severe preeclampsia compared with mild preeclampsia and normal pregnancy.

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Plasma P selectin (GMP-140) and glycocalicin are elevated in preeclampsia and eclampsia: Their significances

Cited by 70 publications

References 21 publications

Increase in Glycocalicin Levels in Platelet Concentrates Stored in Plasma or Synthetic Medium for 8 Days: Comparison with Other Platelet Activation Markers

Increase in Glycocalicin Levels in Platelet Concentrates Stored in Plasma or Synthetic Medium for 8 Days: Comparison with Other Platelet Activation Markers

P-selectin deficiency exacerbates experimental glomerulonephritis: a protective role for endothelial P-selectin in inflammation

A comparative study of vascular cell adhesion molecule-1 and high-sensitive C-reactive protein in normal and preeclamptic pregnancies

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