Plasma-on-chip device for stable irradiation of cells cultured in media with a low-temperature atmospheric pressure plasma

Okada, T.; Chang, Chun-Yao; Kobayashi, Mime; Shimizu, Tetsuji; Sasaki, Minoru; Kumagai, Shinya

doi:10.1016/j.abb.2016.04.001

Cited by 11 publications

(12 citation statements)

References 28 publications

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“…High-voltage square pulses (with a fixed voltage of 7 kV p-p , peak to peak, and a fixed frequency of 10 kHz) were applied to the electrode. For comparison, the typical operation conditions of the Plasma-on-Chip device were 0.5–1.0 slpm of He and 0.5–1.0 kV at a frequency of 1–10 kHz 33 34 . In the cases involving the Plasma-on-Chip , or those using NTAPPJ and the through-hole devices, reactive species were generated by the interactions between the emerging He plasma and the ambient air.…”

Section: Resultsmentioning

confidence: 99%

“…It is readily understood that the plasma affects cells in the vicinity of the treated area. Figure 1 illustrates what occurs when the plasma irradiation area is reduced to a size as small as a cell (plasma ≒ cell) or smaller (plasma < cell) 30 31 32 33 34 . In these cases, we expect that the plasma treatment activates or inactivates biological reactions at the single-cell level.…”

mentioning

confidence: 99%

“…We have developed a plasma cell culture device that enables plasma treatment at the single-cell level ( Fig. 2 ) 32 33 34 . The device is referred to as “ Plasma-on-Chip ” and is used to analyse the interactions between the plasma and cells in real time.…”

mentioning

confidence: 99%

“…If the size of the through-hole is reduced to smaller than that of a cell, the cell is locally treated with plasma, which can selectively activate or inactivate the specific functions of the cell. Thus far, we have reported the inactivation of Chlorella cells with the Plasma-on-Chip device 32 34 …”

mentioning

confidence: 99%

“…To analyse the plasma-cell interaction in more detail, we should know the behaviour of the reactive species during treatment. In the gas phase, optical emission spectroscopic studies showed that the plasma can generate elements to form RONS, including excited O* atoms, excited N 2 molecules, and OH radicals 32 34 43 . Ionic species were also identified by ambient molecular beam mass spectrometry 44 45 46 .…”

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confidence: 99%

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Plasma cell treatment device Plasma-on-Chip: Monitoring plasma-generated reactive species in microwells

Kojima

Sasaki

et al. 2017

Sci Rep

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We have developed a plasma cell treatment device called Plasma-on-Chip that enables the real-time monitoring of a single cell culture during plasma treatment. The device consists of three parts: 1) microwells for cell culture, 2) a microplasma device for generating reactive oxygen and nitrogen species (RONS) for use in cell treatment, and 3) through-holes (microchannels) that connect each microwell with the microplasma region for RONS delivery. Here, we analysed the delivery of the RONS to the liquid culture medium stored in the microwells. We developed a simple experimental set-up using a microdevice and applied in situ ultraviolet absorption spectroscopy with high sensitivity for detecting RONS in liquid. The plasma-generated RONS were delivered into the liquid culture medium via the through-holes fabricated into the microdevice. The RONS concentrations were on the order of 10–100 μM depending on the size of the through-holes. In contrast, we found that the amount of dissolved oxygen was almost constant. To investigate the process of RONS generation, we numerically analysed the gas flow in the through-holes. We suggest that the circulating gas flow in the through-holes promotes the interaction between the plasma (ionised gas) and the liquid, resulting in enhanced RONS concentrations.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

mentioning

confidence: 99%

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Plasma cell treatment device Plasma-on-Chip: Monitoring plasma-generated reactive species in microwells

Kojima

Sasaki

et al. 2017

Sci Rep

Self Cite

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Plasma‐on‐Chip: device for non‐thermal atmospheric pressure plasma irradiation to single cells

Kumagai

Kobayashi

Shimizu

et al. 2020

Elect Comm in Japan

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Plasma Applications in Microalgal Biotechnology

Nguyen

Scarsini

Poncin‐Epaillard

et al. 2022

Agritech: Innovative Agriculture Using Microwaves and Plasmas

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Microalgae are very important organisms for the biosphere because they are at the basis of most of the food chains. Some taxa can also bloom and release dangerous toxins in the environment.They are also very promising for industrial applications in various sectors, including food, feed, pharmaceutical, wellness, energy, building, space, water treatment, biosensing, biotechnology etc. The development of these applications require in many cases the optimization of processing steps.Plasma technology has been already applied on biomolecules, proteins, enzymes, peptides in the biomedical field, for the preparation of bioactive, antifouling surfaces of biomaterials. Nowadays, the research prospective deals with the agriculture domain in the purpose of cleaning, sterilizing or fertilizing the soil. However, plasma technology could also be explored in the case of bioenergetic cells where the plasma chemistry enhances bioadhesion and, bioproduction without destroying the microalgae. This chapter gives illustrations on this new application of plasma technology.

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Plasma-on-chip device for stable irradiation of cells cultured in media with a low-temperature atmospheric pressure plasma

Cited by 11 publications

References 28 publications

Plasma cell treatment device Plasma-on-Chip: Monitoring plasma-generated reactive species in microwells

Plasma cell treatment device Plasma-on-Chip: Monitoring plasma-generated reactive species in microwells

Plasma‐on‐Chip: device for non‐thermal atmospheric pressure plasma irradiation to single cells

Plasma Applications in Microalgal Biotechnology

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