Plasma cell treatment device Plasma-on-Chip: Monitoring plasma-generated reactive species in microwells

Abstract

We have developed a plasma cell treatment device called Plasma-on-Chip that enables the real-time monitoring of a single cell culture during plasma treatment. The device consists of three parts: 1) microwells for cell culture, 2) a microplasma device for generating reactive oxygen and nitrogen species (RONS) for use in cell treatment, and 3) through-holes (microchannels) that connect each microwell with the microplasma region for RONS delivery. Here, we analysed the delivery of the RONS to the liquid culture medium stored in the microwells. We developed a simple experimental set-up using a microdevice and applied in situ ultraviolet absorption spectroscopy with high sensitivity for detecting RONS in liquid. The plasma-generated RONS were delivered into the liquid culture medium via the through-holes fabricated into the microdevice. The RONS concentrations were on the order of 10–100 μM depending on the size of the through-holes. In contrast, we found that the amount of dissolved oxygen was almost constant. To investigate the process of RONS generation, we numerically analysed the gas flow in the through-holes. We suggest that the circulating gas flow in the through-holes promotes the interaction between the plasma (ionised gas) and the liquid, resulting in enhanced RONS concentrations.

A Comparison of Floating-Electrode DBD and kINPen Jet: Plasma Parameters to Achieve Similar Growth Reduction in Colon Cancer Cells Under Standardized Conditions

“…Modeling studies suggest that plasma in direct contact with a target alters species deposition and cell membrane oxidation [101][102][103]. Experimentally, plasma-derived oxidants have been shown to be deposited on cells and liquids through plasma-treated agarose membranes [104] and micropores [105], which may facilitate the deciphering effects of jet plasma short-lived species in cells.…”

Recent advances in microfluidic devices for single-cell cultivation: methods and applications

“…The membranes are implemented to squeeze the air out and diffuse the electrolysis-generated bubbles from the chamber, which are useful for the next solution introduction and cell cultivation. 133,134 Moreover, the membrane is installed between the PDMS plate and waste tank, inducing medium exchange during medium flow. 135…”

“…Microfluidic devices for single-cell interaction assays have been employed to elicit fundamental cellular mechanisms between individual cells, such as cancer cells, 17,134,[192][193][194] immune cells, 15,[195][196][197] bacterial cells, 198 neurons, 50 stem Fig. 6 Probing cellular phenotypes using single-cell cultivation-based microfluidic devices.…”

“…cells, 199 and virus-infected cells. 200 These interactions can be identified by cell-cell contact labelling, 15,50 metabolite production, 17,134,197 cell proliferation and migration, 17,134,157,194,199 and material exchange. 198 Microfluidic setups to conduct single-cell interaction assays are usually developed with fluidic force-incorporated confinement arrays and multi-microchambers separated by channels.…”

Effect of sliding discharge on proliferation and death of brain microvessel endothelial cells in vitro

“…(например, для формирования сосудистой сети с заданной архитектоникой для применения в качестве импланта) [5] или задач моделирования гистогематических барьеров in vitro (например, модели гематоэнцефалического барьера, модели гломерулярного фильтра in vitro), пригодных для тестирования новых лекарственных препаратов или при конструировании микрофизиологических систем в формате "орган-на-чипе" или "ткань-на-чипе". В этом контексте особенно интересны недавние результаты японских исследователей, разработавших систему "плазма-на-чипе" на основе неравновесной плазмы для мониторинга в режиме реального времени состояния культивируемых клеток [6].…”