Plasma MicroRNAs Relate to Atrial Fibrillation Recurrence after Catheter Ablation: Longitudinal Findings from the MiRhythm Study

Vaze, Aditya; Donahue, Kevin; Spring, Matthew; Sardana, Mayank; Tanrıverdi, Kahraman; Freedman, Jane E.; Keaney, John F.; Benjamin, Emelia J.; Lubitz, Steven A.; Rosenthal, Lawrence; Floyd, Kevin; McManus, David D.

doi:10.4172/2155-9880.1000502

Cited by 6 publications

(5 citation statements)

References 32 publications

(58 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The circulating miR-21 level is also associated with AF ablation results in patients with persistent AF undergoing ablation (and this level correlates with left atrial low voltage areas) 8 . Other studies have shown higher levels of miRNAs 125a, 10b, 601, 30a-3p, and 199b in patients with 12-month AF recurrences after catheter ablation than in patients without AF recurrences 9 .…”

Section: Discussionmentioning

confidence: 82%

Serum microRNA in patients undergoing atrial fibrillation ablation

Kiliszek

Maciak

Maciejak

et al. 2020

Sci Rep

View full text Add to dashboard Cite

MicroRNAs mediate posttranscriptional gene regulation. The aim of the study was to find a microRNA predictor of successful atrial fibrillation (AF) ablation. A total of 109 patients undergoing first-time AF ablation were included. Nineteen patients were selected to undergo serum microRNA sequencing (study group). The sequencing data were used to select several microRNAs that correlated with 12month recurrences after AF ablation. Those microRNAs were validated by digital droplet PCR in samples from remaining 90 patients. All patients underwent pulmonary vein isolation (RF ablation, contact force catheter, electroanatomical system). The endpoint of the study was the 12-month AF recurrence rate; the overall recurrence rate was 42.5%. In total, levels of 34 miRNAs were significantly different in sera from patients with Af recurrence compared to patients without Af recurrence. Six microRnAs (miR-183-5p, miR-182-5p, miR-32-5p, miR-107, miR-574-3p, and miR-144-3p) were validated in the whole group. Data from the validation group did not confirm the observations from the study group, as no significant differences were found between miRNAs serum levels in patients with and without recurrences 12 months after AF ablation.

show abstract

Section: Discussionmentioning

confidence: 82%

Serum microRNA in patients undergoing atrial fibrillation ablation

Kiliszek

Maciak

Maciejak

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…miRNAs regulate the molecular processes that trigger the structural and electrophysiological remodeling key to the development of AF (6–9, 21). They are associated with incident and recurrent AF, and their dynamic nature after catheter ablation suggests that they are related to the pathogenesis of AF (5, 15, 21). In this work, we explore the relationship between iLAEAT and plasma miRNA and hypothesize their potential role in creating a substrate for AF.…”

Section: Discussionmentioning

confidence: 99%

“…As part of the miRhythm study examining links between circulating miRNAs and AF, 584 participants were recruited at the University of Massachusetts Medical Center (UMMC) between April 2011 to July 2017 (15). Study protocol was approved by the University of Massachusetts Institutional Review Board (IRB #14875).…”

Section: Methodsmentioning

confidence: 99%

Micro-RNAs Are Related to Epicardial Adipose Tissue in Participants With Atrial Fibrillation: Data From the MiRhythm Study

Tran

Majka

Sanghai

et al. 2019

Front. Cardiovasc. Med.

Self Cite

View full text Add to dashboard Cite

Introduction: Epicardial adipose tissue (EAT) has been linked to incidence and recurrence of atrial fibrillation (AF), but the underlying mechanisms that mediate this association remain unclear. Circulating microRNAs (miRNAs) contribute to the regulation of gene expression in cardiovascular diseases, including AF. Thus, we sought to test the hypothesis that circulating miRNAs relate to burden of EAT. Methods: We examined the plasma miRNA profiles of 91 participants from the miRhythm study, an ongoing study examining associations between miRNA and AF. We quantified plasma expression of 86 unique miRNAs commonly expressed in cardiomyocytes using quantitative reverse transcriptase polymerase chain reaction (qPCR). From computed tomography, we used validated methods to quantify the EAT area surrounding the left atrium (LA) and indexed it to body surface area (BSA) to calculate indexed LA EAT (iLAEAT). Participants were divided into tertiles of iLAEAT to identify associations with unique miRNAs. We performed logistic regression analyses adjusting for factors associated with AF to examine relations between iLAEAT and miRNA. We performed further bioinformatics analysis of miRNA predicted target genes to identify potential molecular pathways are regulated by the miRNAs. Results: The mean age of the participants was 59 ± 9, 35% were women, and 97% were Caucasian. Participants in the highest tertile of iLAEAT were more likely to have hypertension, heart failure, and thick posterior walls. In regression analyses, we found that miRNAs 155-5p ( p < 0.001) and 302a-3p ( p < 0.001) were significantly associated with iLAEAT in patients with AF. The predicted targets of the miRNAs identified were implicated in the regulation of cardiac hypertrophy, adipogenesis, interleukin-8 (IL-8), and nerve growth factor (NGF) signaling. Conclusion: miRNA as well as EAT have previously been linked to AF. Our finding that iLAEAT and miRNAs 155-5p and 302a-3p are associated suggest a possible direct link to between these entities in the development and maintenance of AF. Further research is needed to study causal relationships between these biomarkers.

show abstract

“…TBX5 is a target gene of both miR-10a and miR-10b [84]. Expression of miR-10b was found to be upregulated in LA biopsies from patients with chronic AF and valvular heart disease [27] and miR-10b plasma levels are decreased in AF patients after catheter ablation [85]. In the porcine model, shortlasting atrial tachyarrhythmia was associated with significant upregulation of both miR-10a and miR-10b coupled with decreased TBX5 expression in the LA of paced animals that may be permissive for AF development [17].…”

Section: Transcription Factors-microrna Co-regulation In Atrial Fibrimentioning

confidence: 99%

Interplay between cardiac transcription factors and non-coding RNAs in predisposing to atrial fibrillation

Михайлов

Torrado

2018

J Mol Med

View full text Add to dashboard Cite

There is growing evidence that putative gene regulatory networks including cardio-enriched transcription factors, such as PITX2, TBX5, ZFHX3, and SHOX2, and their effector/target genes along with downstream non-coding RNAs can play a potentially important role in the process of adaptive and maladaptive atrial rhythm remodeling. In turn, expression of atrial fibrillation-associated transcription factors is under the control of upstream regulatory non-coding RNAs. This review broadly explores gene regulatory mechanisms associated with susceptibility to atrial fibrillation-with key examples from both animal models and patients-within the context of both cardiac transcription factors and non-coding RNAs. These two systems appear to have multiple levels of cross-regulation and act coordinately to achieve effective control of atrial rhythm effector gene expression. Perturbations of a dynamic expression balance between transcription factors and corresponding non-coding RNAs can provoke the development or promote the progression of atrial fibrillation. We also outline deficiencies in current models and discuss ongoing studies to clarify remaining mechanistic questions. An understanding of the function of transcription factors and non-coding RNAs in gene regulatory networks associated with atrial fibrillation risk will enable the development of innovative therapeutic strategies.

show abstract

Plasma MicroRNAs Relate to Atrial Fibrillation Recurrence after Catheter Ablation: Longitudinal Findings from the MiRhythm Study

Cited by 6 publications

References 32 publications

Serum microRNA in patients undergoing atrial fibrillation ablation

Serum microRNA in patients undergoing atrial fibrillation ablation

Micro-RNAs Are Related to Epicardial Adipose Tissue in Participants With Atrial Fibrillation: Data From the MiRhythm Study

Interplay between cardiac transcription factors and non-coding RNAs in predisposing to atrial fibrillation

Contact Info

Product

Resources

About