Plasma Membranes and Mitochondrial Equivalents as Functionally Co ordinated Structures

Mudd, Stuart; Kawata, Tomio; Payne, John I.; Sall, Theodore; Takagi, Atsushi

doi:10.1038/189079a0

Cited by 19 publications

(9 citation statements)

References 21 publications

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“…13,14) similar to that seen in most of Kellenbergcr and Ryter's illustrations (6,7,19). A difference between these seemingly randomly organized nucleoplasms and those with a higher degree of orderliness in fibrillar organization (5) is that in the former there is more space between the fibers than in the latter.…”

Section: Observationssupporting

confidence: 66%

“…2 to 4), or as an aggregate of small circular profiles (Figs. 6,7,8,13,14). That these bodies are situated inside the nuclear area is suggested by those sections of bacteria in which they are seen surrounded by nucleoplasm.…”

Section: Observationsmentioning

confidence: 99%

“…But the organelles have also been observed partly or completely surrounded by the cytoplasm (Figs. 8-10, 12,13).…”

Section: Observationsmentioning

confidence: 99%

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SOME FEATURES OF A REMARKABLE ORGANELLE IN BACILLUS SUBTILIS

Iterson

1961

The Journal of Cell Biology

108

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In thin sections of Bacillus subtilis certain organelles are observed situated either in the nuclear area from where they can extend into the cytoplasm, or in contact with the cell wall. Inside the nuclear area, the organelle is sometimes composed of concentric layers each seen to consist of two dense borders with a lighter interspace. In other instances, inside as well as outside the nuclear area, the organelles appear as clusters of delicately delimited vesicles. A typical site of occurrence is on the inner rim of the centripetally developing transverse septa, where it appears as a so called peripheral body (2). The micrographs strongly suggest that when the organelles are attached to the walls they have a function in cell wall formation.

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Section: Observationssupporting

confidence: 66%

Section: Observationsmentioning

confidence: 99%

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SOME FEATURES OF A REMARKABLE ORGANELLE IN BACILLUS SUBTILIS

Iterson

1961

The Journal of Cell Biology

108

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“…With the aid of tetrazolium compounds in particular, but also with the Nadi reagent, Janus Green B, and potassium tellurite, Mudd et al (24)(25)(26)(27)(28)(29)(30)(31) described circumscribed local sites where oxidative-reductive events were found to take place. Weibull (47) pointed out for light microscopy that vital staining of bacteria with tetrazolium cannot be relied upon to reveal the intracellular localization of centres of reducing activity; on the electron microscopic level, there is even less certainty that the formazan is at the site of its formation.…”

Section: Discussionmentioning

confidence: 99%

A Cytochemical Localization of Reductive Sites in a Gram-Positive Bacterium

Iterson

Leene

1964

The Journal of Cell Biology

109

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In bacteria the exact location of a respiratory enzyme system comparable to that of the mitochondria of other cells has remained uncertain. On the one hand, the existence of particulate "bacterial mitochondria" has been advocated (Mudd); on the other hand, important enzymes of the respiratory chain were recovered in the cytoplasmic membranes associated with some granular material (Weibull). In order to gain insight into this question, sites of reducing activity were localized in thin sections of bacteria using the reduction of potassium tellurite as .an indicator. When this salt was added to the culture medium of Bacillus subtilis, it turned out that in this Gram~positive organism the reduced product is strictly bound at two sites, and that the plasma membrane does not materially gain in electron opacity through deposition of the reduced product. The reduction product is found on or in the membranes of particular organelles, which may possibly be regarded as the mitochondrial equivalents in Gram-positlve bacteria, and which are sometimes seen connected to the plasma membrane. The second location is in thin rod-like elements at the cell periphery, possibly the sites from which the flagella emerge.

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“…However, by treating them with detergents or other surfaceactive reagents such as deoxycholic acid (Hunt, 1959), which disassociate lipid and protein, the enzymes sometimes may be brought into solution and further purified. Granules which reduce tetrazolium in whole cells have been regarded as equivalent to animal and plant mitochondria.These have been identified with theisolated lipoprotein granules as 'bacterial mitochondria' (Mudd, Kawata, Payne, Sall & Takagi, 1961). There seems little doubt now, however, that most if not all of such preparations have been derived by comminution of the cytoplasmic membrane from which they can be readily prepared by, for instance, treatment with ultra-sound .…”

mentioning

confidence: 99%

The Bacterial Cytoplasmic Membrane

Hughes

1962

Journal of General Microbiology

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Plasma Membranes and Mitochondrial Equivalents as Functionally Co ordinated Structures

Cited by 19 publications

References 21 publications

SOME FEATURES OF A REMARKABLE ORGANELLE IN BACILLUS SUBTILIS

SOME FEATURES OF A REMARKABLE ORGANELLE IN BACILLUS SUBTILIS

A Cytochemical Localization of Reductive Sites in a Gram-Positive Bacterium

The Bacterial Cytoplasmic Membrane

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