Plasma membrane fatty acid-binding protein and mitochondrial glutamic-oxaloacetic transaminase of rat liver are related.

Berk, Paul D.; Wada, Hiroshi; Horio, Yoshiyuki; Potter, Barry J.; Sorrentino, Dario; Zhou, Shengli; Isola, Luis; Stump, Decherd; Kiang, Chih-Li; Thung, Swan N.

doi:10.1073/pnas.87.9.3484

Cited by 142 publications

(109 citation statements)

References 43 publications

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“…3 As recently reviewed, 6 the former of these has proven identical to the mitochondrial isoform of aspartate aminotransferase (mAspAT). [39][40][41] Nevertheless, its presence on adipocyte and hepatocyte plasma membranes has been firmly established by immunofluorescence, immunohistochemical, and immunoelectron microscopic techniques and by immunoprecipitation from highly purified plasma membrane preparations, 6 while its function as a plasma membrane LCFA transporter was demonstrated in antibody inhibition, 28 transfection, 42 and microinjection 43 experiments. Relative mRNA levels in these published studies were assayed principally by Northern hybridization analysis.…”

Section: Discussionmentioning

confidence: 99%

Long-chain fatty acid uptake is upregulated in omental adipocytes from patients undergoing bariatric surgery for obesity

et al. 2004

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OBJECTIVE:To determine the impact of obesity on adipocyte cell size and long-chain fatty acid (LCFA) uptake kinetics in human subjects undergoing laparoscopic abdominal surgery. SUBJECTS: A total of 10 obese patients (BMI 49.8711.9 (s.d.) kg/m 2 ) undergoing laparoscopic bariatric surgery, and 10 nonobese subjects (BMI 24.272.3 kg/m 2 ) undergoing other clinically indicated laparoscopic abdominal surgical procedures. MEASUREMENTS: Cell size distribution and [ 3 H]oleic acid uptake kinetics were studied in adipocytes isolated from omental fat biopsies obtained during surgery. Adipocyte surface area (SA) was calculated from the measured cell diameters. Plasma leptin and insulin concentrations were measured by RIA in fasting blood samples obtained on the morning of surgery. RESULTS: The mean SA of obese adipocytes (41 50875381 m 2 /cell) was increased 2.4-fold compared to that of nonobese adipocytes (16 92876529 m 2 /cell; Po0.01). LCFA uptake in each group was the sum of saturable and nonsaturable components. Both the V max of the saturable component (21.376.3 vs 5.171.9 pmol/s/50 000 cells) and the rate constant k of the nonsaturable component (0.01570.002 vs 0.006670.0023 ml/s/50 000 cells) were increased (Po0.001) in obese adipocytes compared with nonobese controls. When expressed relative to cell size, V max /m 2 SA was greater in obese than nonobese adipocytes (Po0.05), whereas k/m 2 SA did not differ between the groups. CONCLUSION: The data support the concepts that (1) adipocyte LCFA uptake consists of distinct facilitated (saturable) and diffusive processes; (2) increased saturable LCFA uptake in obese adipocytes is not simply a consequence of increased cell size, but rather reflects upregulation of a facilitated transport process; and (3) the permeability of adipocyte plasma membranes to LCFA is not appreciably altered by obesity, and increased nonsaturable uptake in obese adipocytes principally reflects an increase in cell SA. Regulation of saturable LCFA uptake by adipocytes may be an important control point for body adiposity.

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Section: Discussionmentioning

confidence: 99%

Long-chain fatty acid uptake is upregulated in omental adipocytes from patients undergoing bariatric surgery for obesity

et al. 2004

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“…[8][9][10][20][21][22][23] Fatty acid translocase 31 and fatty acid transport protein 32 are additional putative transporters of FFA which are expressed principally in adipocytes and skeletal and/or cardiac myocytes. Differentiating pre-adipocyte cell lines provide a useful model for examining the expression of proposed FFA transporters in adipose tissue, and the resultant effects on FFA transport.…”

Section: Discussionmentioning

confidence: 99%

“…4 Increased levels of AspAT in plasma, comprised principally of mAspAT, are characteristic of ethanol (EtOH) abuse 5,6 and are widely believed to reflect leakage from damaged mitochondria observed in the hepatocytes of severe alcoholics. 6,7 The demonstration that mAspAT is identical to plasma membrane fatty acid binding protein, 8,9 a putative transporter of longchain free fatty acids (FFA), 10,11 raises the possibility that the hepatocyte plasma membrane, rather than or in addition to mitochondria, might be the source of the increased levels of mAspAT found in the plasma of alcoholics.…”

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Ethanol up-regulates fatty acid uptake and plasma membrane expression and export of mitochondrial aspartate aminotransferase in HepG2 cells

Zhou¹,

Gordon²,

Stump³

et al. 1998

Hepatology

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“…Therefore, FAs are obtained chiefly from the intestine and liver in the form of chylomicrons and very-low-density lipoproteins, respectively (Mossab et al, 2002). The free FAs are released by lipoprotein lipase and then transported by several membrane proteins, such as FA transport protein-1 (FATP-1), membrane FA-binding protein (mFABP), FA translocase (FAT/CD36) and caveolin-1 (Berk et al, 1990;Trigatti et al, 1991;Harmon and Abumrad, 1993;Schaffer and Lodish, 1994). In the context of such a multiprotein-mediated process, whether these transporters selectively transport various FA across the cell membrane remains to be determined.…”

Section: Introductionmentioning

confidence: 99%

Selective transport of long-chain fatty acids by FAT/CD36 in skeletal muscle of broilers

Guo

Shu

Zhou

et al. 2013

Animal

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Fatty acid translocase (FAT/CD36) is a membrane receptor that facilitates long-chain fatty acid uptake. To investigate its role in the regulation of long-chain fatty acid composition in muscle tissue, we studied and compared FAT/CD36 gene expression in muscle tissues of commercial broiler chickens and Chinese local Silky fowls. The results from gas chromatography-mass spectrometry analysis of muscle samples demonstrated that Chinese local Silky fowls had significantly higher (P , 0.05) proportions of linoleic acid (LA) and palmitic acid, lower proportions (P , 0.05) of arachidonic acid (AA) and oleic acid than the commercial broiler chickens. The mRNA expression levels of fatty acid (FA) transporters (FA transport protein-1, membrane FA-binding protein, FAT/CD36 and caveolin-1) in the m. ipsilateral pectoralis and biceps femoris were analyzed by Q-PCR, and FAT/CD36 expression levels showed significant differences between these types of chickens (P , 0.01). Interestingly, the levels of FAT/CD36 expression are positively correlated with LA content (r 5 0.567, P , 0.01) but negatively correlated with palmitic acid content (r 5 20.568, P , 0.01). Further experiments in the stably transfected Chinese hamster oocytes cells with chicken FAT/CD36 cDNA demonstrated that overexpression of FAT/CD36 improves total FA uptake with a significant increase in the proportion of LA and AA, and a decreased proportion of palmitic acid. These results suggest that chicken FAT/CD36 may selectively transport LA and AA, which may lead to the higher LA deposition in muscle tissue.

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Plasma membrane fatty acid-binding protein and mitochondrial glutamic-oxaloacetic transaminase of rat liver are related.

Cited by 142 publications

References 43 publications

Long-chain fatty acid uptake is upregulated in omental adipocytes from patients undergoing bariatric surgery for obesity

Long-chain fatty acid uptake is upregulated in omental adipocytes from patients undergoing bariatric surgery for obesity

Ethanol up-regulates fatty acid uptake and plasma membrane expression and export of mitochondrial aspartate aminotransferase in HepG2 cells

Selective transport of long-chain fatty acids by FAT/CD36 in skeletal muscle of broilers

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