Selective transport of long-chain fatty acids by FAT/CD36 in skeletal muscle of broilers

Abstract

Fatty acid translocase (FAT/CD36) is a membrane receptor that facilitates long-chain fatty acid uptake. To investigate its role in the regulation of long-chain fatty acid composition in muscle tissue, we studied and compared FAT/CD36 gene expression in muscle tissues of commercial broiler chickens and Chinese local Silky fowls. The results from gas chromatography-mass spectrometry analysis of muscle samples demonstrated that Chinese local Silky fowls had significantly higher (P , 0.05) proportions of linoleic acid (LA) and palmitic acid, lower proportions (P , 0.05) of arachidonic acid (AA) and oleic acid than the commercial broiler chickens. The mRNA expression levels of fatty acid (FA) transporters (FA transport protein-1, membrane FA-binding protein, FAT/CD36 and caveolin-1) in the m. ipsilateral pectoralis and biceps femoris were analyzed by Q-PCR, and FAT/CD36 expression levels showed significant differences between these types of chickens (P , 0.01). Interestingly, the levels of FAT/CD36 expression are positively correlated with LA content (r 5 0.567, P , 0.01) but negatively correlated with palmitic acid content (r 5 20.568, P , 0.01). Further experiments in the stably transfected Chinese hamster oocytes cells with chicken FAT/CD36 cDNA demonstrated that overexpression of FAT/CD36 improves total FA uptake with a significant increase in the proportion of LA and AA, and a decreased proportion of palmitic acid. These results suggest that chicken FAT/CD36 may selectively transport LA and AA, which may lead to the higher LA deposition in muscle tissue.

Tribbles expression in cumulus cells is related to oocyte maturation and fatty acid metabolism

“…The up-regulation of CD36 in etomoxir-treated CC may be explained by the possibly increased amounts of saturated FA (such as palmitic acid) as a result of etomoxir inhibition of CPT1 activity which blocks FA entry into mitochondria. This is in line with the studies that showed that palmitic acid induced a rise in CD36 mRNA in bovine mammary cell culture [51] or in chicken skeletal muscle [52]. Moreover, CD36 is a mediator of inflammation, including that which responds to excess fat supply [53].…”

Transcriptome Analysis of Post-Hatch Breast Muscle in Legacy and Modern Broiler Chickens Reveals Enrichment of Several Regulators of Myogenic Growth

“…Six gene transcripts that function in acyl oxidation are enriched in the D6 Ross 708 muscle: CD36, acyl-CoA dehydrogenase 9 (ACAD9), acyl-CoA dehydrogenase , long chain (ACADL) and acyl-CoA dehydrogenase short/branched chain (ACADSB), 3-oxoacid CoA transferase 1 (OXCT1), and carnitine palmitoyltransferase 2 (CPT2). CD36 is a class B scavenger receptor that functions as a fatty acid transporter in chicken skeletal muscle [ 43 ]. CD36 localizes to the plasma membrane and mitochondria; at the plasma membrane CD36 functions in fatty acid uptake, whereas in the mitochondria it functions to import fatty acids for oxidation [ 43 ].…”

LCFA Uptake and FAT/CD36: molecular cloning, tissue expression and mRNA expression responses to dietary oil sources in grass carp (Ctenopharyngodon idellus)

“…(Jump 2004; reviewed by Glatz and Luiken 2017). Initially, it was considered that LCFAs were taken up by cells through passive diffusion mechanisms (Kamp et al 2003;Pownall and Hamilton 2003;Hamilton and Brunaldi 2007), while evidence has shown that LCFA uptake is also facilitated by a proteinmediated mechanism Bonen et al 2007;Guo et al 2013). In this sense, the intracellular transport of free FAs in mammals is facilitated by specific low-molecularweight and highly conserved cytoplasmic proteins that bind both long-chain FAs and other hydrophobic ligands.…”