Plasma Levels of hsa-miR-619-5p and hsa-miR-1184 Differ in Prostatic Benign Hyperplasia and Cancer

Knyazev, E N; Fomicheva, K. A.; Михайленко, Д. С.; Nyushko, K. M.; Samatov, Timur R.; Alekseev, B. Ya.; Shkurnikov, M. Yu.

doi:10.1007/s10517-016-3357-7

Cited by 32 publications

(21 citation statements)

References 14 publications

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“…Published studies demonstrate that miR-1184 (expressed at the highest levels in our high-risk cohort), is located on the X chromosome but has not been studied in breast cancer. Circulating levels of miR-1184 are reported to be increased in patients with prostate cancer [ 42 ], but were decreased in breast cancer cases in our cohort. MicroRNA-4423-3p has a role in regulating epithelial cell differentiation [ 43 ], is reduced in lung tumors [ 43 ], and downregulated in rheumatic heart disease [ 44 ].…”

Section: Discussionmentioning

confidence: 73%

Development of a predictive miRNA signature for breast cancer risk among high-risk women

et al. 2017

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Significant limitations exist in our ability to predict breast cancer risk at the individual level. Circulating microRNAs (C-miRNAs) have emerged as measurable biomarkers (liquid biopsies) for cancer detection. We evaluated the ability of C-miRNAs to identify women most likely to develop breast cancer by profiling miRNA from serum obtained long before diagnosis. 24 breast cancer cases and controls (matched for risk and age) were identified from women enrolled in the High-Risk Breast Program at the UVM Cancer Center. Isolated RNA from serum was profiled for over 2500 human miRNAs. The miRNA expression data were input into a stepwise linear regression model to discover a multivariable miRNA signature that predicts long-term risk of breast cancer. 25 candidate miRNAs were identified that individually classified cases and controls based on statistical methodologies. A refined 6-miRNA risk-signature was discovered following regression modeling that distinguishes cases and controls (AUC0.896, CI 0.804-0.988) in this cohort. A functional relationship between miRNAs that cluster together when cases are contrasted against controls was suggested and confirmed by pathway analyses. The discovered 6 miRNA risk-signature can discriminate high-risk women who ultimately develop breast cancer from those who remain cancer-free, improving current risk assessment models. Future studies will focus on functional analysis of the miRNAs in this signature and testing in larger cohorts. We propose that the combined signature is highly significant for predicting cancer risk, and worthy of further screening in larger, independent clinical cohorts.

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Section: Discussionmentioning

confidence: 73%

Development of a predictive miRNA signature for breast cancer risk among high-risk women

et al. 2017

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“…Connections between miRNA expression and the development of various diseases have been established. miRNA concentrations change in cancer [8] and cardiovascular diseases [9]. Metabolic perturbations change miRNA concentrations in cells [10].…”

Section: Introductionmentioning

confidence: 99%

The Binding Sites of miR-619-5p in the mRNAs of Human and Orthologous Genes

et al. 2017

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BackgroundNormally, one miRNA interacts with the mRNA of one gene. However, there are miRNAs that can bind to many mRNAs, and one mRNA can be the target of many miRNAs. This significantly complicates the study of the properties of miRNAs and their diagnostic and medical applications.ResultsThe search of 2,750 human microRNAs (miRNAs) binding sites in 12,175 mRNAs of human genes using the MirTarget program has been completed. For the binding sites of the miR-619-5p the hybridization free energy of the bonds was equal to 100% of the maximum potential free energy. The mRNAs of 201 human genes have complete complementary binding sites of miR-619-5p in the 3’UTR (214 sites), CDS (3 sites), and 5’UTR (4 sites). The mRNAs of CATAD1, ICA1L, GK5, POLH, and PRR11 genes have six miR-619-5p binding sites, and the mRNAs of OPA3 and CYP20A1 genes have eight and ten binding sites, respectively. All of these miR-619-5p binding sites are located in the 3’UTRs. The miR-619-5p binding site in the 5’UTR of mRNA of human USP29 gene is found in the mRNAs of orthologous genes of primates. Binding sites of miR-619-5p in the coding regions of mRNAs of C8H8orf44, C8orf44, and ISY1 genes encode the WLMPVIP oligopeptide, which is present in the orthologous proteins. Binding sites of miR-619-5p in the mRNAs of transcription factor genes ZNF429 and ZNF429 encode the AHACNP oligopeptide in another reading frame. Binding sites of miR-619-5p in the 3’UTRs of all human target genes are also present in the 3’UTRs of orthologous genes of mammals. The completely complementary binding sites for miR-619-5p are conservative in the orthologous mammalian genes.ConclusionsThe majority of miR-619-5p binding sites are located in the 3’UTRs but some genes have miRNA binding sites in the 5’UTRs of mRNAs. Several genes have binding sites for miRNAs in the CDSs that are read in different open reading frames. Identical nucleotide sequences of binding sites encode different amino acids in different proteins. The binding sites of miR-619-5p in 3’UTRs, 5’UTRs and CDSs are conservative in the orthologous mammalian genes.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3811-6) contains supplementary material, which is available to authorized users.

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“… 20 Significant decrease hsa-let-7c-5p was detected in prostatic cancer. 21 QRT-PCR analysis showed that hsa-let-7c-5p was down-regulated in grade 3 endometrial cancer. 22 Let-7c showed a down-regulated trend in cervical intraepithelial lesions.…”

Section: Discussionmentioning

confidence: 98%

<p>Comprehensive Analysis of circRNA-miRNA-mRNA Network in Cervical Squamous Cell Carcinoma by Integrated Analysis</p>

Liu

Lao

et al. 2020

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Plasma Levels of hsa-miR-619-5p and hsa-miR-1184 Differ in Prostatic Benign Hyperplasia and Cancer

Cited by 32 publications

References 14 publications

Development of a predictive miRNA signature for breast cancer risk among high-risk women

Development of a predictive miRNA signature for breast cancer risk among high-risk women

The Binding Sites of miR-619-5p in the mRNAs of Human and Orthologous Genes

<p>Comprehensive Analysis of circRNA-miRNA-mRNA Network in Cervical Squamous Cell Carcinoma by Integrated Analysis</p>

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