Plasma levels and diagnostic utility of VEGF, MMP-9, and TIMP-1 in the diagnosis of patients with breast cancer

Ławicki, Sławomir; Głażewska, Edyta Katarzyna; Będkowska, Grażyna Ewa; Szmitkowski, Maciej

doi:10.2147/ott.s99959

Cited by 38 publications

(43 citation statements)

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“…Unfortunately, we could not compare our data regarding MMP-7 and tissue inhibitor of metalloproteinase −1 with other publications. A positive correlation between TIMP-1 and CA15–3 concentrations in a group including 100 breast cancer patients (stages I-IV) ( R = 0.28) has also been previously revealed [39]. Some authors have demonstrated significant positive correlations between CA125 and HE4 levels in patients with ovarian malignancies ( R = 0.54) [44, 45].…”

Section: Discussionmentioning

confidence: 69%

Plasma levels of MMP-7 and TIMP-1 in laboratory diagnostics and differentiation of selected histological types of epithelial ovarian cancers

et al. 2017

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BackgroundMMP-7 and TIMP-1 may play a role in the pathogenesis of cancer disease. In this study we investigated plasma levels of selected metalloproteinase and its tissue inhibitor in comparison to plasma levels of the commonly accepted tumor markers (CA 125 and HE4) in selected histological types of epithelial ovarian cancer patients as compared to control groups: patients with a benign ovarian tumor and healthy subjects. Plasma levels of MMP-7 and TIMP-1 were determined using ELISA, CA 125 and HE4 – by CMIA methods.ResultsPlasma levels of all biomarkers studied were significantly higher in ovarian cancer patients as compared to both control groups. MMP-7 demonstrated comparable to HE4 or CA125 values of diagnostic sensitivity (SE: 61%; 68%; 58%, respectively), specificity (SP: 95%; 95%; 98%, respectively), positive (PPV: 93%; 96%; 98%, respectively) and negative predictive values (NPV: 61%; 66%; 60%, respectively) in the groups tested. The combined use of the aforementioned biomarkers resulted in a further increase in diagnostic criteria and AUC, especially in the early stages of the disease.ConclusionsThese findings suggest the usefulness of combining MMP-7 with CA 125 and HE4 in the diagnosis of epithelial ovarian cancer as a new tumor marker panel.Electronic supplementary materialThe online version of this article (doi:10.1186/s13048-017-0338-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 69%

Plasma levels of MMP-7 and TIMP-1 in laboratory diagnostics and differentiation of selected histological types of epithelial ovarian cancers

et al. 2017

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“…) is in the range of other optical label-free platforms making use of surface waves, it is still larger than previously reported plasma VEGF concentrations in healthy humans and cancer patients, ranging from a few tens to a few hundreds of pg/ml (Iinuma, 2010;Jelkmann, 2001;Ławicki et al, 2016). We calibrated the operation in the fluorescence detection mode by setting the minimum concentration measured in label-free mode as the largest for fluorescence.…”

Section: Despite the Lodlf (And γmentioning

confidence: 99%

Bloch surface wave label-free and fluorescence platform for the detection of VEGF biomarker in biological matrices

Rizzo

Alvaro

Danz

et al. 2018

Sensors and Actuators B: Chemical

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We report on the detection of an angiogenic molecule Vascular Endothelial Growth Factor (VEGF) in different biological matrices by means of a new integrated biosensing platform exploiting the properties of Bloch surface waves. The new platform takes advantage of a tandem configuration, in which both label-free and enhanced fluorescence detection are implemented. Specifically designed one dimensional photonic crystals were deposited directly on disposable and low cost plastic biochips. A direct sandwich immunoassay was used to detect VEGF in buffer, cell culture supernatant and human plasma at low concentration (ng/mL). The platform enabled the detection of VEGF in all three matrices with high resolution, fast turnaround time (30 min) and in close agreement with the results of enzyme linked immunosorbent assays

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“…In addition, AUC increased to 0.938 (0.903–0.973). Compared with other studies of combined diagnostic indicators, our results exhibit a clinical advantage 5,50…”

Section: Discussionmentioning

confidence: 44%

“…Carbohydrate antigen 153 is a tumor marker for breast cancer, although its diagnostic sensitivity and specificity are limited 14,15. Recently, new indicators for diagnosing breast cancer have been studied, including free DNA in the plasma, microRNA, long-chain non-coding RNA, and vascular endothelial growth factor 5,16–21…”

Section: Introductionmentioning

confidence: 99%

Diagnostic value of PD-1 mRNA expression combined with breast ultrasound in breast cancer patients

Fang

Shao

et al. 2018

TCRM

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IntroductionThis study explored the value of measuring programmed death 1 (PD-1) in peripheral blood, combined with breast ultrasound using the Breast Imaging Reporting and Data System (BI-RADS) classification, for differentiation between benign and malignant breast tumors.Materials and methodsWe enrolled 113 patients with breast cancer and 66 patients with benign breast tumors who were admitted to Hangzhou First People’s Hospital from September 2014 to August 2017. The mRNA level of PD-1 was detected by quantitative real-time polymerase chain reaction.ResultsThe mRNA levels of PD-1 were significantly higher in the peripheral blood of patients with breast cancer than those in patients with benign breast tumors. The diagnostic sensitivity of PD-1 mRNA expression was 0.805, the specificity was 0.788, and the area under the curve (AUC) was 0.848 (P < 0.001); the sensitivity of breast ultrasound-based BI-RADS classification was 0.752, the specificity was 0.909, and the AUC was 0.906 (P < 0.001); and the combined sensitivity, specificity, and AUC of the two assays were 0.920, 0.879, and 0.938, respectively (P < 0.001). Progesterone receptor-positive breast cancer patients exhibited high levels of PD-1 expression (P < 0.001).ConclusionThis study suggests that the measurement of PD-1 combined with breast ultrasound-based BI-RADS classification represents a significant improvement for breast cancer diagnosis compared with diagnoses based on either method alone.

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Plasma levels and diagnostic utility of VEGF, MMP-9, and TIMP-1 in the diagnosis of patients with breast cancer

Cited by 38 publications

References 56 publications

Plasma levels of MMP-7 and TIMP-1 in laboratory diagnostics and differentiation of selected histological types of epithelial ovarian cancers

Plasma levels of MMP-7 and TIMP-1 in laboratory diagnostics and differentiation of selected histological types of epithelial ovarian cancers

Bloch surface wave label-free and fluorescence platform for the detection of VEGF biomarker in biological matrices

Diagnostic value of PD-1 mRNA expression combined with breast ultrasound in breast cancer patients

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