Bloch surface wave label-free and fluorescence platform for the detection of VEGF biomarker in biological matrices

Rizzo, Riccardo; Alvaro, Maria; Danz, Norbert; Napione, Lucia; Descrovi, Emiliano; Schmieder, Stefan; Sinibaldi, Alberto; Chandrawati, Rona; Rana, Subinoy; Munzert, Peter; Schubert, Thomas; Maillart, Emmanuel; Anopchenko, Aleksei; Rivolo, Paola; Mascioletti, Alessandro; Sonntag, Frank; Stevens, Molly M.; Bussolino, Federico; Michelotti, Francesco

doi:10.1016/j.snb.2017.09.018

Cited by 27 publications

(40 citation statements)

References 35 publications

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“…The optical layout of the platform was designed to operate either detecting the angular reflectance at λ 1 or exploiting the BSW field enhancement when exciting at λ 2 and detecting BSW coupled fluorescence, peaked around λ 1, from DyLight 650 labelled antibodies in a sandwich Ang2 immunoassay [10]. However, the estimated limit of detection (LoD) of the label-free operation (about 11 ng/mL) is not sufficient for the detection of Ang2 at very low and clinically relevant concentrations, whereas the estimated LoD of the fluorescence mode (about 1 ng/mL) falls within the relevant range [27]. Therefore, in the present work we carried out Ang2 detection assays in the fluorescence mode only.…”

Section: Read-out Systemmentioning

confidence: 99%

“…In Fig. 1(a), we show a photograph of a biochip [27] together with its microfluidic cover. The substrate includes an integrated coupling prism operating in the Kretschmann-Raether configuration under total internal reflection (TIR) conditions.…”

Section: Bsw Biochipsmentioning

confidence: 99%

“…1 [10,25]. The shape of the emission profile is related to the DyLight 650 emission spectrum via the dispersion of the TE and TM polarized BSW; for a given 1DPC design, it is therefore a characteristic signature of such a dye [27].…”

Section: Immunoassay Formatmentioning

confidence: 99%

“…Here, we present the detection of both Ang2 spiked in buffer and endogenous Ang2 biomarker directly from human blood plasma, with the sensing platform operating in the fluorescence mode only. While several BSW based biosensors [19,22,[24][25][26][27] were reported for the detection of different analytes, their accuracy was never tested with real clinical samples. Blood plasma, together with serum, is one of the main sources of clinical analytes [28].…”

Section: Introductionmentioning

confidence: 99%

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Bloch surface wave enhanced biosensor for the direct detection of Angiopoietin-2 tumor biomarker in human plasma

Rizzo¹,

Alvaro²,

Danz³

et al. 2018

Biomed. Opt. Express

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Quantitative detection of angiogenic biomarkers provides a powerful tool to diagnose cancers in early stages and to follow its progression during therapy. Conventional tests require trained personnel, dedicated laboratory equipment and are generally time-consuming. Herein, we propose our developed biosensing platform as a useful tool for a rapid determination of Angiopoietin-2 biomarker directly from patient plasma within 30 minutes, without any sample preparation or dilution. Bloch surface waves supported by one dimensional photonic crystal are exploited to enhance and redirect the fluorescence arising from a sandwich immunoassay that involves Angiopoietin-2. The sensing units consist of disposable and low-cost plastic biochips coated with the photonic crystal. The biosensing platform is demonstrated to detect Angiopoietin-2 in plasma samples at the clinically relevant concentration of 6 ng/mL, with an estimated limit of detection of approximately 1 ng/mL. This is the first Bloch surface wave based assay capable of detecting relevant concentrations of an angiogenic factor in plasma samples. The results obtained by the developed biosensing platform are in close agreement with enzyme-linked immunosorbent assays, demonstrating a good accuracy, and their repeatability showed acceptable relative variations.

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Section: Read-out Systemmentioning

confidence: 99%

Section: Bsw Biochipsmentioning

confidence: 99%

Section: Immunoassay Formatmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bloch surface wave enhanced biosensor for the direct detection of Angiopoietin-2 tumor biomarker in human plasma

Rizzo¹,

Alvaro²,

Danz³

et al. 2018

Biomed. Opt. Express

Self Cite

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show abstract

“…In addition to optical fibres and planar waveguides, 1-dimensional photonic crystals are also emerging as the optically active substrate for EW sensing. For example, Rizzo et al developed a Blotch surface wave-based label-free sensor for the detection of vascular endothelial growth factor, a well-known cancer biomarker [177]. The assay was complete within 30 minutes and the LOD in human serum was 3.5 ng mL −1 .…”

Section: Evanescent Wave-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

Recent Progress in Optical Sensors for Biomedical Diagnostics

Pirzada

Altıntaş

2020

Micromachines

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In recent years, several types of optical sensors have been probed for their aptitude in healthcare biosensing, making their applications in biomedical diagnostics a rapidly evolving subject. Optical sensors show versatility amongst different receptor types and even permit the integration of different detection mechanisms. Such conjugated sensing platforms facilitate the exploitation of their neoteric synergistic characteristics for sensor fabrication. This paper covers nearly 250 research articles since 2016 representing the emerging interest in rapid, reproducible and ultrasensitive assays in clinical analysis. Therefore, we present an elaborate review of biomedical diagnostics with the help of optical sensors working on varied principles such as surface plasmon resonance, localised surface plasmon resonance, evanescent wave fluorescence, bioluminescence and several others. These sensors are capable of investigating toxins, proteins, pathogens, disease biomarkers and whole cells in varied sensing media ranging from water to buffer to more complex environments such as serum, blood or urine. Hence, the recent trends discussed in this review hold enormous potential for the widespread use of optical sensors in early-stage disease prediction and point-of-care testing devices.

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Bioassay engineering: a combined label-free and fluorescence approach to optimize HER2 detection in complex biological media

et al. 2020

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Bloch surface wave label-free and fluorescence platform for the detection of VEGF biomarker in biological matrices

Cited by 27 publications

References 35 publications

Bloch surface wave enhanced biosensor for the direct detection of Angiopoietin-2 tumor biomarker in human plasma

Bloch surface wave enhanced biosensor for the direct detection of Angiopoietin-2 tumor biomarker in human plasma

Recent Progress in Optical Sensors for Biomedical Diagnostics

Bioassay engineering: a combined label-free and fluorescence approach to optimize HER2 detection in complex biological media

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