Plasma leptin levels in pigs with different leptin and leptin receptor genotypes

Abstract

A C3469T mutation at exon 3 of the pig leptin (Lep) gene has been genotyped in diverse pig breeds yielding controversial results with regard to its association with growth, fatness and carcass traits. A similar situation has been reported for a HpaII restriction fragment length polymorphism (RFLP) in the pig leptin receptor (Lepr) gene, where associations were found depending on the statistical model employed. The main objective of our work was to investigate if leptin plasma concentrations differ in pigs with different C3469T and Lepr HpaII RFLP genotypes. With this aim, we have measured plasma leptin levels at 160 days in 68 Landrace pigs with different Lep C3469T and Lepr HpaII RFLP genotypes. Neither Lep (TT: 11.68 ng/ml, TC: 10.71 ng/ml) nor Lepr (AA: 12.6 ng/ml, AB: 10.93 ng/ml, BB: 11.74 ng/ml) genotypes influenced significantly plasma Lep concentration. Moreover, we did not find any association between Lep and Lepr genotypes and phenotypic variation at growth and fatness traits in a commercial population of 320 Landrace pigs.

The Effect of Lupinus albus and Calcium Chloride on Growth Performance, Body Composition, Plasma Biochemistry and Meat Quality of Male Pigs Immunized Against Gonadotrophin Releasing Factor

“…The leptin concentrations were also higher in the current experiment compared to McCauley et al [4] and Batorek et al [10]. This can be attributed to differences in the assay type (multi-species radioimmunoassay method versus a pig specific competitive ELISA method) and leptin specificity of the respective methods [23]. …”

Preliminary identification and characterisation of leptin gene polymorphism in Indian goats

“…Several SNPs have been previously reported in introns and exons of the leptin gene among different breeds of cattle (Schenkel et al 2005). Associations of polymorphisms in leptin with carcass and meat quality traits were also reported in Iranian sheep (Barzehkar et al 2009) and exon 3 of pigs (Amills et al 2007). Our results prove the presence of single polymorphic site in exon 2 and intron 2 each, different from the SNP reported by Singh et al (2009).…”

Mapping QTL for fat area ratios and serum leptin concentrations in a Duroc purebred population

“…2004, 2005). In pig populations, LEP and LEPR genes have also been reported as candidate genes for serum leptin concentration (Amills et al . 2008; Kuehn et al .…”

“…(2009) investigated the relationships between the genotypes of the LEP and LEPR genes and serum leptin concentrations in two lines by crossing maternal White cross (Yorkshire × Maternal Landrace) gilts to Duroc or Landrace boars, and in one line by crossing progeny of the Duroc‐ and Landrace‐sired lines, and evidenced an association between the genotype of the LEPR gene and serum leptin concentration and no effect of the LEP gene. Amills et al . (2008) did not determine any association between the genotypes of two genes and serum leptin concentrations in a Landrace population.…”

“…For candidate gene analysis, polymorphisms in the leptin (LEP) and LEPR genes have been found to influence serum leptin concentration in human and cattle populations (Thompson et al 1997;Strobel et al 1998;Liefers et al 2004Liefers et al , 2005. In pig populations, LEP and LEPR genes have also been reported as candidate genes for serum leptin concentration (Amills et al 2008;Kuehn et al 2009). Kuehn et al (2009) investigated the relationships between the genotypes of the LEP and LEPR genes and serum leptin concentrations in two lines by crossing maternal White cross (Yorkshire ¥ Maternal Landrace) gilts to Duroc or Landrace boars, and in one line by crossing progeny of the Duroc-and Landrace-sired lines, and evidenced an association between the genotype of the LEPR gene and serum leptin concentration and no effect of the LEP gene.…”